Enantioselective determination of azelnidipine in human plasma using liquid chromatography–tandem mass spectrometry

Kawabata, Kiyoshi; Samata, Naozumi; Urasaki, Yoko; Fukazawa, Ichiro; Uchida, Naoki; Uchida, Eiji; Yasuhara, Hajime

doi:10.1016/j.jchromb.2007.01.050

Cited by 22 publications

(11 citation statements)

References 14 publications

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“…Commercial AGP columns have been used for the separation of many chiral compounds [154,157–164]. For example, chiral separations have been achieved by using these columns for atenolol, citalopram, warfarin, verapamil, norverapamil, atropine, and vinca alkaloid analogues [157–161].…”

Section: Chromatographic Studies Of Protein-based Cspsmentioning

confidence: 99%

“…One example is ovomucoid, which is a glycoprotein found in egg whites and that contains three homologous domains [118]. Ovomucoid columns have been used for chiral separations of a large number of drugs and chiral solutes [28,118,164,181–184]. In one recent study, an ovomucoid-based CSP was used with LC-MS/MS to separate and analyze the enantiomers of ondansetron and doxazosin in human plasma and to give information on the pharmacokinetics of these agents [185,186].…”

Section: Chromatographic Studies Of Protein-based Cspsmentioning

confidence: 99%

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Chromatographic Studies of Protein-Based Chiral Separations

Zheng

Azaria

et al. 2016

Separations

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The development of separation methods for the analysis and resolution of chiral drugs and solutes has been an area of ongoing interest in pharmaceutical research. The use of proteins as chiral binding agents in high-performance liquid chromatography (HPLC) has been an approach that has received particular attention in such work. This report provides an overview of proteins that have been used as binding agents to create chiral stationary phases (CSPs) and in the use of chromatographic methods to study these materials and protein-based chiral separations. The supports and methods that have been employed to prepare protein-based CSPs will also be discussed and compared. Specific types of CSPs that are considered include those that employ serum transport proteins (e.g., human serum albumin, bovine serum albumin, and alpha1-acid glycoprotein), enzymes (e.g., penicillin G acylase, cellobiohydrolases, and α-chymotrypsin) or other types of proteins (e.g., ovomucoid, antibodies, and avidin or streptavidin). The properties and applications for each type of protein and CSP will also be discussed in terms of their use in chromatography and chiral separations.

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Section: Chromatographic Studies Of Protein-based Cspsmentioning

confidence: 99%

Section: Chromatographic Studies Of Protein-based Cspsmentioning

confidence: 99%

Chromatographic Studies of Protein-Based Chiral Separations

Zheng

Azaria

et al. 2016

Separations

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“…In order to obtain a representative plasma for method development and validation, a plasma pool was prepared by proportionally mixing six plasmas obtained from different healthy volunteers [59,60].…”

Section: Standard Solutions and Spiked Plasma Samplesmentioning

confidence: 99%

Validation of a fast liquid chromatography–UV method for the analysis of drugs used in combined cardiovascular therapy in human plasma

Iriarte

González

Ferreiròs

et al. 2009

Journal of Chromatography B

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“…Exploring a stable and reliable method for chiral separation still remains one of the greatest challenges in the last decades for pharmaceutical analysis, especially in the development of new chiral drugs. Many separation technologies such as capillary electrophoresis (CE) [7,8], supercritical fluid chromatography (SFC) [9], capillary electrochromatography (CEC) [10], gas chromatography (GC) [11], and high-performance liquid chromatography (HPLC) [12,13,14,15] have been developed for obtaining efficient chiral separation for DHP enantiomers. Among all these separation techniques, HPLC remains predominant in the analytical scale enantioseparation arena [16].…”

Section: Introductionmentioning

confidence: 99%

Direct Enantiomeric Resolution of Seventeen Racemic 1,4-Dihydropyridine-Based Hexahydroquinoline Derivatives by HPLC

Sun

Gündüz

Zhang

et al. 2019

IJMS

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1,4-Dihydropyridine (DHP) scaffold holds an outstanding position with its versatile pharmacological properties among all heterocyclic compounds. Although most of the commercially available DHPs are marketed as a racemic mixture, the chiral center at C-4 can lead to even opposite pharmacological activities between the enantiomers. In the present study, enantioseparation of seventeen DHP structural analogues, consisting of either pharmacologically active or newly synthesized derivatives, (M2-4, MD5, HM2, HM10, CE5, N11, N10, N7, M11, MC6-8, MC13, MD23, and 42IIP) by high-performance liquid chromatography was investigated using immobilized polysaccharide-based chiral stationary phase, Chiralpak IC column. Due to the solvent versatility of the covalently immobilized chiral stationary phase in enantiomer separation, multiple elution modes including standard normal phase, nonstandard mobile phase, and reversed phase were used to expand the possibility to find the optimum enantioselective conditions for the tested analytes. Under appropriate separation conditions, complete enantiomeric separation was obtained for nearly all compounds except MC6-8 and MC13 which contained two chiral centers. Additionally, the effects of the polar modifier, the additive, and column temperature on the chiral recognition were evaluated. The thermodynamic parameters calculated according to the linear van’t Hoff equation indicated that the chiral separations in this study were enthalpy-driven or entropy-driven. Some parameters of method validation such as linearity, limit of quantitation, and repeatability were also measured for all studied compounds to prove the reliability of the method.

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Enantioselective determination of azelnidipine in human plasma using liquid chromatography–tandem mass spectrometry

Cited by 22 publications

References 14 publications

Chromatographic Studies of Protein-Based Chiral Separations

Chromatographic Studies of Protein-Based Chiral Separations

Validation of a fast liquid chromatography–UV method for the analysis of drugs used in combined cardiovascular therapy in human plasma

Direct Enantiomeric Resolution of Seventeen Racemic 1,4-Dihydropyridine-Based Hexahydroquinoline Derivatives by HPLC

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