Enantioselective measurement of the Candida metabolite d-arabinitol in human serum using multi-dimensional gas chromatography and a new chiral phase

Wong, Brian; Castellanos, M. C.

doi:10.1016/s0378-4347(00)82606-7

Cited by 30 publications

(19 citation statements)

References 12 publications

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“…The results obtained with several different enantioselective analytical methods supported the hypothesis that measuring D-arabinitol selectively yielded better results than measuring total arabinitol nonselectively (10,178,179,236,239).…”

Section: D-arabinitol In Candida Infectionssupporting

confidence: 61%

Current Status of Nonculture Methods for Diagnosis of Invasive Fungal Infections

2002

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The incidence of invasive fungal infections has increased dramatically in recent decades, especially among immunocompromised patients. However, the diagnosis of these infections in a timely fashion is often very difficult. Conventional microbiologic and histopathologic approaches generally are neither sensitive nor specific, and they often do not detect invasive fungal infection until late in the course of disease. Since early diagnosis may guide appropriate treatment and prevent mortality, there has been considerable interest in developing nonculture approaches to diagnosing fungal infections. These approaches include detection of specific host immune responses to fungal antigens, detection of specific macromolecular antigens using immunologic reagents, amplification and detection of specific fungal nucleic acid sequences, and detection and quantitation of specific fungal metabolite products. This work reviews the current status and recent developments as well as problems in the design of nonculture diagnostic methods for invasive fungal infections

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Section: D-arabinitol In Candida Infectionssupporting

confidence: 61%

Current Status of Nonculture Methods for Diagnosis of Invasive Fungal Infections

2002

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“…Other diagnostic methods that have been studied in these patient groups include the detection of fungal metabolites, such as D-arabinitol (especially when expressed as a ratio with the L-isomer) in disseminated candidiasis (51)(52)(53)(54) or D-mannitol in invasive aspergillosis (55,56). However, these cannot yet be recommended for routine management of neutropenic and organ transplant patients.…”

Section: Fever Of Unknown Originmentioning

confidence: 99%

Guidelines for the investigation of invasive fungal infections in haematological malignancy and solid organ transplantation

Denning

Evans

Kibbler

et al. 1997

Eur. J. Clin. Microbiol. Infect. Dis.

146

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Invasive fungal infections are increasing in incidence and now affect as many as 50% of neutropenic/bone marrow transplant patients and 5 to 20% of solid organ transplant recipients. Unfortunately, many of the diagnostic tests available have a low sensitivity. The guidelines presented here have been produced by a working party of the British Society for Medical Mycology in an attempt to optimise the use of these tests. The yield of fungi from blood cultures can be increased by ensuring that at least 20 ml of blood are taken for aerobic culture, by using more than one method of blood culture, and by employing terminal subculture if continuous monitoring systems are used with a five-day incubation protocol. Skin lesions in febrile neutropenic patients should be biopsied and cultured for fungi. The detection of galactomannan in blood or urine is of value in diagnosing invasive aspergillosis only if tests are performed at least twice weekly in high-risk patients. Antigen detection tests for invasive candidiasis are less valuable. Computed tomography scanning is particularly valuable in diagnosing invasive pulmonary fungal infection when the chest radiograph is negative or shows only minimal changes. Bronchoalveolar lavage is most useful in patients with diffuse changes on computed tomography scan. The major advances in the diagnosis of invasive fungal infection in patients with haematological malignancy or solid organ transplantation have been in the use of imaging techniques, rather than in the development of new mycological methods in the routine laboratory.

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“…However, prospective clinical trials using this test have not been reported. Moreover, detection of fungal metabolites like D-Arabinitol has been proven beneficial for early diagnosis of invasive candidiasis in animal models [20,21]. In nonneutropenic individuals, C. albicans-spedfic IgM anti bodies had a superior sensitivity and specificity compared to the two commercially available Candida antigen assays [22].…”

Section: Candidiasismentioning

confidence: 99%

New Aspects for the Diagnosis of Invasive Fungal Disease in Oncological Patients

Hebart¹,

Bokemeyer²,

Löffler³

et al. 1997

Oncol Res Treat

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In recent years, invasive fungal infections have emerged as an increasing cause of morbidity and mortality in immunocom-promised patients. Early diagnosis of invasive fungal infections remains difficult as the currently available detection systems lack either sensitivity or specificity or both. Therefore, anti-fungal therapy is more often administered for clinically presumed than for proven fungal infection. Culture detection of fungal species from blood is not sensitive enough. Thus, assays for detection of fungal specific circulating antigens have been developed and applied in defined patient populations. Recently, polymerase chain reaction (PCR) assays have emerged as powerful tools with high sensitivity and specificity for the diagnosis of a broad variety of fungal pathogens. PCR has been applied successfully for detection of fungal pathogens in experimental settings, and encouraging preliminary results concerning application of PCR to clinical samples are available. Further prospective trials will help to define the potential clinical value of the new assays discussed.

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Enantioselective measurement of the Candida metabolite d-arabinitol in human serum using multi-dimensional gas chromatography and a new chiral phase

Cited by 30 publications

References 12 publications

Current Status of Nonculture Methods for Diagnosis of Invasive Fungal Infections

Current Status of Nonculture Methods for Diagnosis of Invasive Fungal Infections

Guidelines for the investigation of invasive fungal infections in haematological malignancy and solid organ transplantation

New Aspects for the Diagnosis of Invasive Fungal Disease in Oncological Patients

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