Enantioselective separation of rivastigmine by capillary electrophoresis with cyclodextrines

Lucangioli, Silvia; Trípodi, Valeria; Masrian, Edmond; Scioscia, Silvia L.; Carducci, C. N.; Kenndler, Ernst

doi:10.1016/j.chroma.2005.02.038

Cited by 14 publications

(8 citation statements)

References 18 publications

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“…Among articles dealing with the determination of interaction constants of various analytes with charged CDs, methanol seems to be the most popular EOF marker . The other often used neutral markers are: mesityl oxide (MO) , DMSO , thiourea (TU) , nitromethane (NM) , ethanol , and acetone .…”

Section: Introductionmentioning

confidence: 99%

Determination of effective mobilities of EOF markers in BGE containing sulfated β‐cyclodextrin by a two‐detector method

et al. 2013

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A neutral marker of the EOF can gain a nonzero effective mobility because of its possible interaction with a charged complexing agent, such as a chiral selector in CE. We determined effective mobilities of four compounds often used as EOF markers (dimethyl sulfoxide, mesityl oxide, nitromethane, and thiourea) in the BGE-containing sulfated β-CD (60 g/L). All the compounds studied were measurably mobilized by their interaction with the selector. The highest effective mobility (-3.0·10(-9) m(2) s(-1) V(-1)) was observed for thiourea and the lowest (-1.5·10(-9) m(2) s(-1) V(-1)) for dimethyl sulfoxide and nitromethane. The mobilities were determined by a new two-detector pressure mobilization method (2d method), which we propose, and the results were confirmed by standard CE measurements. In the 2d method, one marker zone is situated in the BGE containing the charged selector, while the second marker zone is surrounded with a selector-free BGE, which prevents its complexation. The initial distance between the two marker zones equals the capillary length from the inlet to the first detector. After a brief voltage application, the final distance between the marker zones is determined based on known capillary length from the first to the second detector. The difference between these two distances determines the effective mobility.

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Section: Introductionmentioning

confidence: 99%

Determination of effective mobilities of EOF markers in BGE containing sulfated β‐cyclodextrin by a two‐detector method

et al. 2013

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“…In the present method, the LOQ is approximately 1.6 times lower than that of the literatures. 22,23 Finally, the method was applied to determine the optical purity of a nonracemic rivastigmine sample with the ee value of 99.14% (shown in Figure 5). LOQ (S/N＝10) 0.0014 mg/mL LOD (S/N＝3) 0.0004 mg/mL a n is numbers of determinations; S/N is the ratio of sign to noise, Injection volume was calculated as 3 nL; y is peak area, x isconcentration in mg/mL, number of concentration points is 6, each point was measured in triplicate.…”

Section: Methods Validationmentioning

confidence: 99%

Sensitive Method for Enantioseparation of Rivastigmine with Highly Sulfated Cyclodextrin as Chiral Selector by Capillary Electrophoresis

Wang

et al. 2006

Chin. J. Chem.

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A sensitive method for enantioseparation of a basic drug rivastigmine and determination of its optical impurity by capillary electrophoresis with highly sulfated β-cyclodextrin (HS-β-CD) as the chiral selector is described. In general, enantioseparation of basic chiral compounds is carried out in acidic condition (pH 2.5) to prevent analytes from adsorption on the capillary wall. However, in the case of rivastigmine, the detection sensitivity was too limited to determine the optical impurity of S-rivastigmine lower than 1% when buffer pH was 2.5. It was found that the detection sensitivity was improved 1.6 times just by raising the buffer pH value from 2.5 to 5.8. The poor column efficiency due to the adsorption of the analytes on the capillary wall was resolved by using dynamical coating of the capillary wall with the linear polyacrylamide solution. The experimental parameters such as the concentration of HS-β-CD, buffer pH and buffer ionic strength were optimized, respectively. The method was validated in terms of repeatability, linearity, limit of detection (LOD) and limit of quantitation (LOQ). Using the present method, the optical purity of nonracemic rivastigmine with the enantiomeric excess (ee) value of 99.14% was determined.

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“…The use of the CDs with longest tradition, the natural cyclodextrins ␤-CD and ␥-CD, was reported in several quantitative methods summarized in Table 1 [6][7][8][9]. Especially in [10], ␤-CD and ␥-CD were used in Hadamard transform capillary electrophoresis (HTCE) for the separation of glutamic acid enantiomers by a CE-LIF method, with the analyte being labelled by a fluorescent agent.…”

Section: Neutral Cyclodextrinsmentioning

confidence: 99%

Recent advances in pharmaceutical applications of chiral capillary electrophoresis

Hoogmartens

Schepdael

2006

Journal of Pharmaceutical and Biomedical Analysis

108

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Enantioselective separation of rivastigmine by capillary electrophoresis with cyclodextrines

Cited by 14 publications

References 18 publications

Determination of effective mobilities of EOF markers in BGE containing sulfated β‐cyclodextrin by a two‐detector method

Determination of effective mobilities of EOF markers in BGE containing sulfated β‐cyclodextrin by a two‐detector method

Sensitive Method for Enantioseparation of Rivastigmine with Highly Sulfated Cyclodextrin as Chiral Selector by Capillary Electrophoresis

Recent advances in pharmaceutical applications of chiral capillary electrophoresis

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