Enantioseparation and determination of dufulin enantiomers in cucumber and soil by chiral liquid chromatography–tandem mass spectrometry

Abstract

A simple and rapid method for enantioselective determination of dufulin in cucumber and soil was developed by liquid chromatography with tandem mass spectrometry. The enantiomers were separated on a Superchiral S-OD chiral cellulose tris(3,5-dimethylphenylcarbamate) column at 20°C, with a mixture of acetonitrile and water (0.1% formic acid; 52:48, v/v) as mobile phase at a flow rate of 0.65 mL/min. The pretreatment conditions were optimized using an orthogonal test, and the optimized method showed good linearity and sensitivity. The limits of detection and limits of quantification of two dufulin enantiomers were 0.006 and 0.02 mg/kg, respectively. The average recoveries of S-enantiomer and R-enantiomer in cucumber and soil were 80.61-99.83% and 80.97-102.96%, respectively, with relative standard deviations of 1.30-9.72%. The method was successfully applied to determine dufulin in real cucumber and soil samples. The results demonstrate that the method could facilitate further research on the differences between individual dufulin enantiomers with respect to metabolites and environmental fate and finally help reveal the complex interactions that exist between dufulin, humans and the environment.

Enantioselective determination of phenthoate enantiomers in plant‐origin matrices using reversed‐phase high‐performance liquid chromatography–tandem mass spectrometry

“…The matrix‐dependent LOD for both enantiomers was defined as the concentration that produced a signal‐to‐noise ratio of 3 when analyzing samples spiked with phenthoate enantiomers (Xia et al, 2019; Zhang et al, 2020a). The LOQ is considered as the lowest fortified concentration verified by recovery experiments, which were conducted to evaluate the accuracy and precision of the new method (Zhang et al, 2018; Zhang et al, 2019).…”

Oxidative Stress and Enantioselective Degradation of Dufulin on Tubifex

“…The S-(+)-enantiomer is hydrolyzed faster than its antipode. Zhang et al investigated the enantioselective degradation of dufulin in 4 types of soil, and their results indicate that enantiopure S-(+)-dufulin degraded much more quickly than its antipode R-(−)-dufulin in all nonsterile soils (Zhang KK et al 2013Zhang HZ et al 2018). Wang et al (2014) studied the adsorption-desorption and degradation of dufulin in soil, and their results show that the adsorption coefficients (K f ) of dufulin in 3 soils were 13.71, 10.40, and 7.98, respectively.…”