Enantioseparation of Peptides with Two Chiral Centers by Capillary Electrophoresis

Shen, Gangyi; She, Donglai; Yu, Wanting; Li, Shuwen

doi:10.1109/icbeb.2012.196

Cited by 1 publication

(3 citation statements)

References 11 publications

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“…Shen et al. resolved Ala‐Met, Ala‐Leu, Ala‐Val, and Ala‐Leu‐Gly using a combination of β‐CD and sodium dioxycholate.…”

Section: Chiral Separation Of Small Peptidesmentioning

confidence: 99%

“…Besides SDS, sodium deoxycholate was used by Chen et al [177] in combination with 2-hydroxypropyl-␤-CD for the stereoisomeric separation of Tyr-Phe, Tyr-Leu, and Ala-Gln derivatized with naphthalene-2,3-dicarboxyaldehyde. Shen et al [178] resolved Ala-Met, Ala-Leu, Ala-Val, and Ala-Leu-Gly using a combination of ␤-CD and sodium dioxycholate.…”

Section: Micellar Electrokinetic Chromatographymentioning

confidence: 99%

“…It consists of Tyr-Phe, Tyr-Leu and Ala-Gln derivatized with naphthalene-2,3dicarboxyaldehyde 2-Hydroxypropyl-␤-CD SDS, sodium deoxycholate [177] 11. Ala-Met, Ala-Leu, Ala-Val and Ala-Leu-Gly ␤-CD Sodium dioxycholate [178] derivatization of an analyte with a chiral reagent to produce a diastereomeric mixture, which can be separated using an achiral MEKC. The different approaches to the indirect technique were reviewed [10].…”

Section: Micellar Electrokinetic Chromatographymentioning

confidence: 99%

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Advances in chiral separations of small peptides by capillary electrophoresis and chromatography

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Many chemical and biological processes are controlled by the stereochemistry of small polypeptides (di-, tri-, tetra-, penta-, hexapeptides, etc). The biological importance of peptide stereoisomers is of great value. Therefore, the chiral resolution of peptides is an important issue in biological and medicinal sciences and drug industries. The chiral resolutions of peptide racemates have been discussed with the use of capillary electrophoresis and chromatographic techniques. The various chiral selectors used were polysaccharides, cyclodextrins, Pirkle types, macrocyclic antibiotics, crown ethers, imprinted polymers, etc. The stereochemistry of dipeptides is also discussed. Besides, efforts are made to explain the chiral recognition mechanisms, which will be helpful in understanding existing and developing new stereoselective analyses. Future perspectives of enantiomeric resolution are also predicted. Finally, the review concludes with the demand of enantiomeric resolution of all naturally occurring and synthetic peptides.

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“…Shen et al. resolved Ala‐Met, Ala‐Leu, Ala‐Val, and Ala‐Leu‐Gly using a combination of β‐CD and sodium dioxycholate.…”

Section: Chiral Separation Of Small Peptidesmentioning

confidence: 99%

Section: Micellar Electrokinetic Chromatographymentioning

confidence: 99%