Enantioseparation of two antifungal azole drugs by analytical countercurrent chromatography using sulfobutyl ether-β-cyclodextrin as chiral selector

Qiu, Huiyun; Xiang, Haiping; Wen, Mengyi; Chen, Songlin; Zhu, Jun; Tong, Shengqiang

doi:10.1016/j.chroma.2023.464185

Cited by 6 publications

(2 citation statements)

References 34 publications

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“…According to the literature [16], the separation temperature, rotational speed, mobile phase flow rate, and injection volume exert varying degrees of influence on the separation performance of HSCCC. In the case of Voriconazole separation via CCC, elevating the temperature during the process leads to a marginal reduction in the enantioselectivity factor [22]. However, maintaining temperature control at high rotational speeds can pose challenges, potentially resulting in sharp column pulsations and consequent peak broadening.…”

Section: Resultsmentioning

confidence: 99%

“…The n ‐hexane/ethyl acetate/100 mmol/L sodium dihydrogen phosphate buffer (pH = 3.0 containing 50 mmol/L SBE‐ β ‐CD) (1.5:0.5:2, v/v/v) solvent system was used for HSCCC separation of Voriconazole racemate [22]. Prior to use, the solvent mixture was vigorously shaken and well equilibrated in a separatory funnel and left for 24 h. Racemic Voriconazole was dissolved in the lower phase to prepare sample solutions.…”

Section: Methodsmentioning

confidence: 99%

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Continuous chiral separation process for high‐speed countercurrent chromatography established and scaled up: A case of Voriconazole enantioseparation

Sun,

Huang,

Pei

et al. 2024

J of Separation Science

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An efficient method for the continuous separation of Voriconazole enantiomers was developed using sulfobutyl ether‐β‐cyclodextrin (SBE‐β‐CD) as a chiral selector in high‐speed countercurrent chromatography (HSCCC) with different types. The separation was performed using a two‐phase solvent system consisting of n‐hexane/ethyl acetate/100 mmol/L phosphate buffer solution (pH = 3.0, containing 50 mmol/L SBE‐β‐CD) (1.5:0.5:2, v/v/v). A fast and predictable scale‐up process was achieved using an analytical DE HSCCC instrument. The optimized parameters were subsequently applied to a preparative Tauto HSCCC instrument, resulting in consistent separation time and enantiomeric purity, with throughput boosted by a remarkable 11‐fold. Preparative HSCCC successfully separated 506 mg of the racemate, delivering enantiomers exceeding 99% purity as confirmed by high‐performance liquid chromatography analysis. This investigation presents an effective methodology for forecasting the HSCCC scale‐up process and attaining continuous separation of chiral drugs.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%