Enantioseparation of β-blockers labelled with a chiral fluorescent reagent, R(−)-DBD-PyNCS, by reversed-phase liquid chromatography

Enantiomeric separation of d,l‐tryptophan and d,l‐kynurenine by HPLC using pre‐column fluorescence derivatization with R(−)‐DBD‐PyNCS

“…As reported in previous papers, a chiral fluorescent labeling reagent, R(-)-DBD-PyNCS was synthesized for the fluorescence determination of chiral amino compounds such as amino acids, peptides and drugs by reversed-phase HPLC (Toyo'oka et al, 1997(Toyo'oka et al, , 2001Jin et al, 1998Jin et al, , 1999. In the present study, R(-)-DBD-PyNCS was applied to separate simultaneously enantiomers of Trp and KYN on an ODS column.…”

“…However, simultaneous separation of Trp and KYN enantiomers has not yet been achieved. In this paper, we employed an HPLC method using pre-column diastereomer derivatization with a chiral derivatizing reagent, R(-)-DBD-PyNCS (Toyo'oka et al, 1997; were purchased from Tokyo Kasei Co. Ltd (Tokyo, Japan). Acetic acid (AcOH), formic acid (HCO2H), and trifluoroacetic acid (TFA) of HPLC grade were obtained from Wako Pure Chemical Industries (Osaka, Japan).…”

Determination of dl-amino acids, derivatized with R(−)-4-(3-isothiocyanatopyrrolidin-1-yl)-7-(N,N-dimethylaminosulfonyl)-2,1,3-benzoxadiazole, in nail of diabetic patients by UPLC–ESI-TOF-MS

“…The proposed derivatization conditions at 55 • C for 20 min in aqueous acetonitrile containing 1% TEA as the base catalyst were also adopted in the present research. In a previous study, the dl-amino acids in food samples were successfully determined by LC separation and FL detection [25,26]. However, the determination in complex matrices, such as plasma and nails, seems to be fairly difficult by the FL detection.…”

Chiral separation of oxprenolol by affinity electrokinetic chromatography-partial filling technique using human serum albumin as chiral selector

“…Although different chiral selectors have been proposed for the resolution of oxprenolol enantiomers using chromatographic and electrophoretic [11][12][13][14][15][16][17][18][19] methods, the enantioseparation of oxprenolol using human serum albumin is of special interest. HSA is the major protein in blood; therefore enantiomeric drug separation can provide useful information on the pharmacokinetic differentiation of drugs.…”