Enantioseparation using cyclosophoraoses as a novel chiral additive in capillary electrophoresis

Lee, Sanghoo; Jung, Seunho

doi:10.1016/s0008-6215(03)00083-1

Cited by 39 publications

(25 citation statements)

References 21 publications

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“…In spite of these advantages, papers published on the separation of T 4 enantiomers by CE are scarce. Only Lee and Jung reported a novel chiral additive of cyclosophoraoses for the separation of T 4 enantiomers in aqueous CE [16].…”

Section: Exists As Two Enantiomeric Forms L-t 4 and D-t 4 (mentioning

confidence: 99%

Enantioselective determination of thyroxine enantiomers by ligand‐exchange CE with UV absorbance and ICP‐MS detection

et al. 2009

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A simple CE method has been developed for the separation and determination of thyroxine (T 4 ) enantiomers in pharmaceutical formulations. The method was based on ligand-exchange mechanism using a Cu(II)/L-proline complex as chiral selector. The effects of different parameters affecting separation such as chiral selector concentration, organic additive, buffer pH and temperature were investigated. A baseline separation of the two enantiomers was obtained at a Cu(II)/L-proline ratio of 1:8 in a borate buffer (15 mmol/L, pH 9.6) containing 10% v/v acetonitrile. Under the optimized conditions, precision linearity range and detection limits of the developed enantioselective CE method were evaluated and compared using two different detection systems: conventional UV detection at 226 nm and iodine ( 127 I)specific detection (''chiral speciation'') with ICP-MS. Both methodologies show adequate analytical performance characteristics with detection limits around 0.30 mg/mL for each enantiomer of T 4 . Finally, a levothroid pharmaceutical formulation sample was successfully analyzed using both developed methods CE-UV and CE-ICP-MS.

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Section: Exists As Two Enantiomeric Forms L-t 4 and D-t 4 (mentioning

confidence: 99%

Enantioselective determination of thyroxine enantiomers by ligand‐exchange CE with UV absorbance and ICP‐MS detection

et al. 2009

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“…Additional studies have indicated that sinorhizobial cyclic β-(1,2)-glucans have the potential for use as commercial complexation agents for a variety of poorly soluble molecules (54)(55)(56)(57)(58)(59). Recent studies have also shown that cyclic β-(1,2)-glucans act as chiral selectors for various enantiomers (60)(61)(62)(63)(64)(65)(66), as catalytic carbohydrates for methanolysis (67)(68)(69), and as morphology-directing agents for the synthesis of selenium nanowires (70). These characteristic functions of PGs may be due to inherent three-dimensional structures, hydrogen-bonding and the complex-forming abilities of the cyclic glucans in aqueous solutions.…”

Section: Novel Physico-chemical Properties Of Pgsmentioning

confidence: 99%

Periplasmic glucans isolated from Proteobacteria

Lee

Cho²,

Jung

2009

BMB Reports

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“…Throughout this interaction, Cys are suspected of being involved in complexation with various plant flavonoids [7]. In addition, neutral or anionic Cys have been applied as host molecules in various technologies of inclusion complexation, for example, as solubility enhancers [8][9][10][11][12] of poorly soluble guest molecules such as luteolin, and as chiral additives in CE [13].…”

Section: Introductionmentioning

confidence: 99%

Separation of some chiral flavonoids by microbial cyclosophoraoses and their sulfated derivatives in micellar electrokinetic chromatography

Park

Jung

2005

Electrophoresis

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Neutral cyclosophoraoses (Cys) and highly sulfated cyclosophoraoses (HS-Cys) were successfully applied as chiral selectors with SDS for the separation of some chiral flavonoids in MEKC. HS-Cys were synthesized by the chemical modification of a family of neutral Cys isolated from a soil microorganism, Rhizobium meliloti 2011. Chiral catechin was separated with a resolution (R(s)) of 0.754 by neutral Cys and SDS. In the case of isosakuranetin and neohesperidin, resolution (R(s)) values of 1.483 and 1.306 were obtained with HS-Cys and SDS, respectively.

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Enantioseparation using cyclosophoraoses as a novel chiral additive in capillary electrophoresis

Cited by 39 publications

References 21 publications

Enantioselective determination of thyroxine enantiomers by ligand‐exchange CE with UV absorbance and ICP‐MS detection

Enantioselective determination of thyroxine enantiomers by ligand‐exchange CE with UV absorbance and ICP‐MS detection

Periplasmic glucans isolated from Proteobacteria

Separation of some chiral flavonoids by microbial cyclosophoraoses and their sulfated derivatives in micellar electrokinetic chromatography

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