Encapsidation and spread of African cassava mosaic virus DNA A in the absence of DNA B when agroinoculated to Nicotiana benthamiana

Klinkenberg, F.A.; Stanley, John

doi:10.1099/0022-1317-71-6-1409

Cited by 67 publications

(31 citation statements)

References 27 publications

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“…These results contrast with those obtained using the pMON200 or pBinl9 binary vectors for agroinoculation where head-to-tail repeats of the virus D N A are required in the vector to allow plant infections (Elmer et al, 1988 a;Hayes et al, 1988). (Klinkenberg & Stanley, 1990). This type of inoculation was inefficient with between 1 0~ and 40 ~ of plants becoming infected and in those plants the amount of D N A A produced was only about 5~o of that associated with full infection following the agroinoculation of both DNAs.…”

Section: Agroinoculation Of Dna a Deletion Mutantscontrasting

confidence: 52%

Mutagenesis of the AC3 open reading frame of African cassava mosaic virus DNA A reduces DNA B replication and ameliorates disease symptoms

Morris

Richardson

Eddy

et al. 1991

Journal of General Virology

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Small insertions were made independently at each of four unique restriction sites on African cassava mosaic virus (ACMV) DNA A to disrupt the three overlapping complementary-sense open reading frames (ORFs) herein designated AC1, AC2 and AC3. The DNA A mutants were assayed for their infectivity by agroinoculation of monomeric constructs to Nicotiana benthamiana plants containing chromosomal insertions of ACMV DNA B. Disruption of the AC30RF alone resulted in a delay and amelioration of disease symptoms which correlated with reduced replication of DNA B. Normal replication of DNA A still carrying the AC30RF mutation was found in extracts from these plants. No ACMV DNA or symptoms were observed in corresponding inoculations with either the simultaneous disruption of the overlapping AC2 and AC30RFs or disruption of the ACI ORF. Complementation by the inoculation of different mutant pairs produced a delay in disease symptoms followed by repair of mutated sites. A DNA A construct with the virus-sense AV1 (coat protein) ORF deleted was infectious producing typical ACMV disease symptoms. A similar construct with a larger deletion encompassing the complementary-sense AC30RF produced symptomless infections. The DNA recovered from plants revealed DNA A of normal size where the position of the deleted ORF was replaced with cloning vector DNA. Significantly reduced DNA B replication was observed for the AC3 deletion construct.

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Section: Agroinoculation Of Dna a Deletion Mutantscontrasting

confidence: 52%

Mutagenesis of the AC3 open reading frame of African cassava mosaic virus DNA A reduces DNA B replication and ameliorates disease symptoms

Morris

Richardson

Eddy

et al. 1991

Journal of General Virology

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“…However, infection by A-DNA alone led to significant disease symptoms. In contrast, plants infected with only the A-DNA of ACMV did not develop symptoms and contained 20-fold less viral DNA than those infected with both A-and B-DNAs (Klinkenberg & Stanley, 1990). More recently it was shown that agrobacteriummediated inoculation of the DNAs of TLCV and two TYLCV isolates on tomato leads to systemic infection indistinguishable from natural whitefly-transmitted disease (Navot et al, 1991 ;Kheyr-Pour et al, 1991 ;, In this regard, TYLCV-Th has characteristics somewhat intermediate among the whitefly-transmitted geminiviruses.…”

Section: Discussionmentioning

confidence: 99%

Complete nucleotide sequence of the geminivirus tomato yellow leaf curl virus, Thailand isolate

Rochester

DePaulo

Fauquet³

et al. 1994

Journal of General Virology

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“…Previously we have shown that, similar to both ACMV and AbMV DNA A (Klinkenberg & Stanley, 1990;Evans & Jeske, 1993a), agroinoculation of N. benthamiana with dimers of the PYMV DNA A component resulted in the independent replication and spread of the DNA in a proportion of the inoculated plants (Buragohain et al, 1994). Biolistic inoculation of N. benthamiana plants with the PYMV DNA A component did not cause systemic infection (Table 1) but small amounts of virus replication in the cells into which the DNA was directly introduced may be undetectable and insufficient to spread from cell-to-cell and initiate a systemic infection.…”

Section: Resultsmentioning

confidence: 99%

“…Following agroinoculation of N. benthamiana plants with dimers of the PYMV genome we demonstrated that both components were required to cause systemic symptoms of * Author for correspondence. Fax +44 17l 584 2056. e-mail r.coutts @ ic.ac.uk infection (Buragohain et al, 1994) but that similar to both African cassava mosaic geminivirus (ACMV) and AbMV (Klinkenberg & Stanley, 1990;Evans & Jeske, 1993a) the PYMV A component replicated and spread independently in a limited fashion in N. benthamiana plants. Mixtures of the monomers or dimers of the complete PYMV genome were not infectious for potato, the natural host of the virus (Buragohain et al, 1994), including the same potato cultivar (Desiree) that was successfully infected by grafting from systemically infected N. benthamiana plants (Roberts et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

Mutational analysis of potato yellow mosaic geminivirus

Sung

Coutts²

1995

Journal of General Virology

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Mutations have been inserted into the virion and complementary sense ORFs encoding proteins with MrS in excess of 9 kDa of both DNA A and DNA B of potato yellow mosaic geminivirus (PYMV). Wild-type and mutant monomeric clones were tested for their ability to replicate, produce PYMV-specific DNA, spread and cause symptoms in Nicotiana benthamiana plants following biolistic inoculation. Dimeric clones of the DNA A mutants were also investigated by agroinoculation of leaf discs. In contrast to N. benthamiana plants agroinoculated with PYMV DNA A, in which the wild-type DNA A component was capable of limited independent replication and spread, both excised DNA A and B components were required for DNA replication and symptom development in plants inoculated by the biolistic method. Mixtures of both genomic components were also infectious for potato plants following biolistic inoculation. Mutations in ORFs ALl, AL2, BR1 and BLl resulted in clones incapable of infecting N. benthamiana plants. However, the AL2 mutation, but not the ALl mutation, allowed viral DNA replication in leaf discs. Mutations to both the ARI and AL30RFs produced clones which were infectious in plants but showed a considerable delay in the production of attenuated symptoms as compared to wild-type infections. Mutating the AL30RF dramatically reduced viral DNA replication in both whole plants and leaf discs. Mutations to the AL40RF produced clones which were as infectious for both N. benthamiana and potato plants as the wild-type clones. Our results are compared with those from mutagenesis studies on related bipartite geminiviruses.

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Encapsidation and spread of African cassava mosaic virus DNA A in the absence of DNA B when agroinoculated to Nicotiana benthamiana

Cited by 67 publications

References 27 publications

Mutagenesis of the AC3 open reading frame of African cassava mosaic virus DNA A reduces DNA B replication and ameliorates disease symptoms

Mutagenesis of the AC3 open reading frame of African cassava mosaic virus DNA A reduces DNA B replication and ameliorates disease symptoms

Complete nucleotide sequence of the geminivirus tomato yellow leaf curl virus, Thailand isolate

Mutational analysis of potato yellow mosaic geminivirus

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