Encapsulated Synbiotic Dietary Supplementation at Different Dosages to Prevent Vibriosis in White Shrimp, Litopenaeus vannamei

Abstract

a b s t r a c t Q 3The aim of this study was to evaluate the effect of encapsulated synbiotic (Bacillus sp. NP5 and oligosaccharide) dietary at different dosages on growth performance, survival rate, feed conversion ratio, and immune responses of Litopenaeus vannamei against Vibrio infection. The shrimps of the main treatments were fed by the diet that contained three different dosages of encapsulated synbiotic [0.5% (A), 1% (B), and 2% (C) (w/w)] with feeding rate of 5% of shrimp biomass (4 times a day). The shrimps of two control treatments (negative control and positive control) were fed only by commercial feed without supplementation of encapsulated synbiotic. The growth, feed conversion ratio, and survival rate were observed after 30 days of encapsulated synbiotic dietary. The shrimps were then challenged by injection of Vibrio harveyi (6 log colony forming units/mL) 0.1 mL/shrimp, excluded the negative control treatment. Afterward, the survival and immune responses were observed for 9 days after experimental infection. The shrimps treated with 2% encapsulated synbiotic (treatment C) in the diet showed the highest growth performance (2.98 ± 0.42%), feed conversion ratio (1.26 ± 0.19), and better immune responses i.e. total hemocyte counts, differential hemocyte count, phenoloxidase, and intestine bacteria observation compared to those of positive control treatment.

Co-Encapsulated Synbiotics and Immobilized Probiotics in Human Health and Gut Microbiota Modulation

“…Despite the well-known extraordinary properties of synbiotics, additional in vivo and clinical trials are essential to demonstrate efficacy, and they need to be sufficiently powered with a randomized placebo control study design. To date, only few animal studies have been done to evaluate the effectiveness of co-encapsulated synbiotics in vivo [249][250][251]. This is particularly important when using microcapsules containing multiple bioactive ingredients, as the positive and negative interactions of synbiotics together with the encapsulation material become paramount to investigate.…”

Supplementation of Nodulisporium sp. KT29 induced by Vibrio harveyi as an immunostimulant for controlling vibriosis in vannamei white shrimp under marine culture system

“…The PO activity is also known as unknown substances detector in humoral non-specific immune system (Li & Xiang, 2013;Hauton, 2012). The increase of PO activity demonstrated that immunostimulant administration was able to induce the resistance and immune system of Litopenaeus vannamei towards V. harveyi infection (Zubaidah et al, 2015;Wahjunigrum et al, 2016).…”

“…One of the most frequently disease which attacked white shrimp is vibriosis caused by Vibrio harveyi (Widanarni et al, 2012). The most common ways to control the disease are using antibiotics (Defoirdt et al, 2011), SPF juvenile selection (Lightner, 2005), using normal ingredients, and sinbiotics which acts as immunostimulant (Sirirustananun et al, 2011;Zubaidah et al, 2015). The antibiotics application was prohibited nowadays because of the food security issue.…”

EFEKTIVITAS KOMBINASI PROBIOTIK MIKROENKAPSULASI MELALUI PAKAN UNTUK PENGENDALIAN PENYAKIT MOTILE AEROMONADS SEPTICEMIA PADA IKAN LELE, Clarias gariepinus

“…Hal ini sesuai dengan pernyataan Zubaidah et al (2015) bahwa semakin tinggi dosis Bacillus sp. yang diberikan maka laju pertumbuhan semakin meningkat.…”