2019
DOI: 10.1101/791442
|View full text |Cite
Preprint
|
Sign up to set email alerts
|

Encapsulation boosts islet-cell signature in differentiating human induced pluripotent stem cells via integrin signalling

Abstract: Cell replacement therapies hold great therapeutic potential. Nevertheless, our knowledge of the mechanisms governing the developmental processes is limited, impeding the quality of differentiation protocols. Generating insulin-expressing cells in vitro is no exception, with the guided series of differentiation events producing heterogeneous cell populations that display mixed pancreatic islet phenotypes and immaturity. The achievement of terminal differentiation ultimately requires the in vivo transplantation … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

1
14
0

Year Published

2020
2020
2020
2020

Publication Types

Select...
3
1

Relationship

2
2

Authors

Journals

citations
Cited by 4 publications
(18 citation statements)
references
References 53 publications
1
14
0
Order By: Relevance
“…The cells were encapsulated in alginate 20 just before the transplantation as alginate (a) allows easy sample recovery, (b) protects against the immune system attack and (c) promotes the islet cell differentiation potential probably through the mechanical forces elicited by encapsulation. 21 Cell viability was over 94% (97.65% ± 0.35 for 1w-postTX and 95.5% ± 1.7 for 4w-postTX). Higher levels of human insulin were detected in the blood of the xenotransplanted hyperglycaemic mice as compared to normoglycaemic hosts, probably because of chronic high glucose stimulation ( Figure S1B) and potentially contributing to the slight drop in glycaemia values observed following transplant ( Figure 1D).…”
Section: Nsg Rat Insulin Promoter-diphtheria Toxin Receptor As An Imentioning
confidence: 93%
“…The cells were encapsulated in alginate 20 just before the transplantation as alginate (a) allows easy sample recovery, (b) protects against the immune system attack and (c) promotes the islet cell differentiation potential probably through the mechanical forces elicited by encapsulation. 21 Cell viability was over 94% (97.65% ± 0.35 for 1w-postTX and 95.5% ± 1.7 for 4w-postTX). Higher levels of human insulin were detected in the blood of the xenotransplanted hyperglycaemic mice as compared to normoglycaemic hosts, probably because of chronic high glucose stimulation ( Figure S1B) and potentially contributing to the slight drop in glycaemia values observed following transplant ( Figure 1D).…”
Section: Nsg Rat Insulin Promoter-diphtheria Toxin Receptor As An Imentioning
confidence: 93%
“…For this study we used 2D differentiation on Matrigel-coated plates until Stage 5 (S5; pancreatic endocrine precursors), or up to Stage 7 (S7; maturing beta-cells), S5 cells were encapsulated in alginate before continued differentiation toward S7, and S5 and S7 cells encapsulated in alginate prior to in vivo transplantation. Alginate encapsulation was performed as we have described earlier (Vethe et al, 2019b). Cell number and viability was measured as previously described (Vethe et al, 2019b).…”
Section: Cell Preparationmentioning
confidence: 99%
“…Alginate encapsulation was performed as we have described earlier (Vethe et al, 2019b). Cell number and viability was measured as previously described (Vethe et al, 2019b). Cell viability mean was 86.53 ± 7.03%.…”
Section: Cell Preparationmentioning
confidence: 99%
See 2 more Smart Citations