Encapsulation enhancement and stabilization of insulin in cationic liposomes

Park, Se Jin; Choi, Soon Gil; Davaa, Enkhzaya; Park, Jeong Sook

doi:10.1016/j.ijpharm.2011.05.061

Cited by 62 publications

(26 citation statements)

References 25 publications

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“…The ability of liposomes to function as sustained release systems can be improved by incorporating charged lipids into the lipid content during liposome preparation. However, the effect of charge on sustained release properties is dependent on the nature of the encapsulated material [28,29]. Thus, the effect of charge on the release properties of curcumin from liposomes was evaluated in this study.…”

Section: In Vitro Releasementioning

confidence: 99%

Effect of Lipid Composition onIn VitroRelease and Skin Deposition of Curcumin Encapsulated Liposomes

Pamunuwa

Karunaratne

2016

Journal of Nanomaterials

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Liposomal encapsulation improves numerous physiochemical and biological properties of curcumin. The aim of this work was to impart slow release and skin delivery of curcumin via liposomal encapsulation. Liposomes were made using egg yolk phosphatidylcholine as the staple lipid while incorporating polysorbate 80 and stearylamine to prepare hybrid liposomes and positively charged liposomes, respectively. Negatively charged liposomes exhibited the highest encapsulation efficiencies (87.8±4.3%) and loading capacities (3.4±0.2%). The sizes of all formulations were about 250 nm, while stearylamine increased the polydispersity index. Positively charged liposomes showed lower degradation temperatures than negatively charged liposomes by 10–15°C, attributable to the presence of stearylamine. The melting temperatures of positively charged liposomes (40–50°C) were much higher than those of negatively charged liposomes (14-15°C), which may have affected release and skin deposition behavior of liposomes. The positively charged liposomes exhibited the slowest release of curcumin in phosphate buffered saline (pH 6.8) and the release profiles of all liposomal formulations conformed to the Gompertz model. The negatively charged liposomes facilitated the highest skin deposition of curcumin as revealed by studies conducted using excised pig ear skin. Concisely, positively and negatively charged liposomes were optimal for slow release and skin deposition of curcumin, respectively.

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Section: In Vitro Releasementioning

confidence: 99%

Effect of Lipid Composition onIn VitroRelease and Skin Deposition of Curcumin Encapsulated Liposomes

Pamunuwa

Karunaratne

2016

Journal of Nanomaterials

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“…49 As a dry powder, CsA is very stable for at least two years under dark and refrigerated (2°C-8°C) conditions. 50 However, liposomes encapsulated with drugs have several problems associated with storage, such as phospholipid hydrolysis and decomposition of encapsulated drug.…”

Section: Chemical Stabilitymentioning

confidence: 99%

Characterization and stability studies of a novel liposomal cyclosporin A prepared using the supercritical fluid method: comparison with the modified conventional Bangham method

Karn

Cho

Park

et al. 2013

IJN

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Abstract:A novel method to prepare cyclosporin A encapsulated liposomes was introduced using supercritical fluid of carbon dioxide (SCF-CO 2 ) as an antisolvent. To investigate the strength of the newly developed SCF-CO 2 method compared with the modified conventional Bangham method, particle size, zeta potential, and polydispersity index (PDI) of both liposomal formulations were characterized and compared. In addition, entrapment efficiency (EE) and drug loading (DL) characteristics were analyzed by reversed-phase high-performance liquid chromatography. Significantly larger particle size and PDI were revealed from the conventional method, while EE (%) and DL (%) did not exhibit any significant differences. The SCF-CO 2 liposomes were found to be relatively smaller, multilamellar, and spherical with a smoother surface as determined by transmission electron microscopy. SCF-CO 2 liposomes showed no significant differences in their particle size and PDI after more than 3 months, whereas conventional liposomes exhibited significant changes in their particle size. The initial yield (%), EE (%), and DL (%) of SCF-CO 2 liposomes and conventional liposomes were 90.98 ± 2.94, 92.20 ± 1.36, 20.99 ± 0.84 and 90.72 ± 2.83, 90.24 ± 1.37, 20.47 ± 0.94, respectively, which changed after 14 weeks to 86.65 ± 0.30, 87.63 ± 0.72, 18.98 ± 0.22 and 75.04 ± 8.80, 84.59 ± 5.13, 15.94 ± 2.80, respectively. Therefore, the newly developed SCF-CO 2 method could be a better alternative compared with the conventional method and may provide a promising approach for large-scale production of liposomes.

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“…Liposomes, with a well biocompatibility and good biodegradability due to the similar structure as cell-surface phospholipid bilayer, have been recently used as a popular nanovesicle for administration of oral drugs including both small molecules and protein owing to its excellent drugloading rate, targeting and slow releasing action as well as enhanced bioavailability (Tan et al, 2010;Huang et al, 2011;Park et al, 2011;Song et al, 2011). In particular, it has been proven that liposomal drugs show better oral bioavailability and long-circulating property (Maud et al, 2003;Guan et al, 2011;Isacchi et al, 2011;Vural et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Long-circulating nanoliposomes (LCNs) sustained delivery of baicalein (BAI) with desired oral bioavailabilityin vivo

Liang¹,

Liu³

et al. 2013

Drug Delivery

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Long-circulating nanoliposomes (LCNs) containing Baicalein (BAI) were prepared by diethyl ether injection method in this paper. Using soybean phosphatidylcholine (SPC) and cholesterol (CHOL) as the carrier ingredients, BAI-loaded LCNs were formulated in optimized condition with encapsulation efficiency (EE) of 41.5 AE 4.77%. The average diameter of BAI-loaded LCNs was $709 nm determined by dynamic light scattering (PDI 0.43). Drug storage stability study showed that BAI-loaded LCNs were highly stable in phosphate-buffered saline (PBS, pH 7.4) at 4 C. Additionally, the pharmacokinetic behaviors of BAI-loaded LCNs were studied using Kunming mice as experimental animals. Parallelly, free BAI solution was studied as control. As expected, it is found that LCNs-encapsulated BAI yields greater BAI oral bioavailability with 4.52 times of free BAI. These results suggest the potential applications of LCNs for the delivery of BAI.

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Encapsulation enhancement and stabilization of insulin in cationic liposomes

Cited by 62 publications

References 25 publications

Effect of Lipid Composition onIn VitroRelease and Skin Deposition of Curcumin Encapsulated Liposomes

Effect of Lipid Composition onIn VitroRelease and Skin Deposition of Curcumin Encapsulated Liposomes

Characterization and stability studies of a novel liposomal cyclosporin A prepared using the supercritical fluid method: comparison with the modified conventional Bangham method

Long-circulating nanoliposomes (LCNs) sustained delivery of baicalein (BAI) with desired oral bioavailabilityin vivo

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