Encapsulation of anticancer drug and magnetic particles in biodegradable polymer nanospheres

Abstract

In this study, we have prepared PLGA (poly-D,L-lactide-co-glycolide) nanospheres loaded with biocompatible magnetic fluid and anticancer drug taxol by a modified nanoprecipitation technique and investigated their magnetic properties. A magnetic fluid, MF-PEG, with a biocompatible layer of polyethylene glycol (PEG), was chosen as a magnetic carrier. The PLGA, whose copolymer ratio of D,L-lactide to glycolide is 85:15, was utilized as a capsulation material. Taxol, as an important anticancer drug, was chosen for its significant role against a wide range of tumours. The morphology and particle size distributions of the prepared nanospheres were investigated by transmission electron microscopy (TEM) and scanning electron microscopy (SEM) and showed a spherical shape of prepared nanospheres with size 250 nm. Infrared spectroscopy (FTIR), differential scanning calorimetry (DSC) and thermogravimetry (TGA) analysis confirmed incorporation of magnetic particles and taxol into the PLGA polymer. The results showed good encapsulation with magnetite content 21.5 wt% and taxol 0.5 wt%. Magnetic properties of magnetic fluids and taxol within the PLGA polymer matrix were investigated by SQUID magnetometry from 4.2 to 300 K. The SQUID measurements showed superparamagnetism of prepared nanospheres with a blocking temperature of 160 K and saturation magnetization 1.4 mT.

The intensity of internalization and cytotoxicity of superparamagnetic iron oxide nanoparticles with different surface modifications in human tumor and diploid lung cells

“…The magnetic nanospheres SO-PEG-PLGA-Fe 3 O 4 were prepared according to Fessi et al [23] with some modifications [24]. In brief, 100 mg of poly(lactide-co-glycolic acid) (PLGA) was dissolved in 10 ml of acetone (the organic phase) and the nanoparticles SO-PEG-Fe 3 O 4 (83 mg Fe 3 O 4 /ml) were mixed with the nonionic detergent, Pluronic F68 (3.2 mg/ml) (the aqueous colloid).…”

Thermal Analysis of Magnetic Polymer Nanospheres for Drug Targeting

“…Magnetic PLGA NPs loaded with different input amounts of taxol and with the mean diameter of 200-250 nm were prepared [4]. Figure 1 shows the relationship between TAX loading and the enthalpy change of TAX melting.…”

“…Taxol (TAX), an important anticancer drug, was chosen for encapsulation to the polymer because of its significant role against a wide range of tumours. Magnetite (Fe 3 O 4 ) was prepared by co-precipitation of ferric and ferrous salts in an alkali aqueous medium [4]. To prepare stable colloidal suspension of magnetic particles, sodium oleate as a first surfactant was used to prevent their agglomeration.…”

“…To prepare stable colloidal suspension of magnetic particles, sodium oleate as a first surfactant was used to prevent their agglomeration. Poly(ethylene glycol) (PEG) as a second surfactant was added to the system magnetite-sodium oleate to improve biocompatibility of magnetic material [4].…”

“…The modified nanoprecipitation method was used for entrapment of magnetic fluid and anticancer drug into polymer nanospheres [4]. After the sphere formulation, the thermal behaviour of the prepared NPs was characterized by DSC and TGA methods.…”