Encapsulation of Bovine Serum Albumin in Temperature‐Programmed “Shell‐in‐Shell” Structures

Abstract

A novel approach to load a hydrophilic bovine serum albumin into drug carriers was proposed in terms of temperature‐programmed “shell‐in‐shell” structures, which were fabricated with poly(N‐isopropylacrylamide), poly(lactide), poly(ethylene glycol), and Au nanoparticles. Spherically well‐defined “shell‐in‐shell” structures were constructed by a modified‐double‐emulsion method (MDEM). The lower critical solubility temperature of the structures was manipulated to 36.4 °C which was confirmed by UV/Vis spectroscopy and DSC (Differential Scanning Calorimetry).TEM image of the Au@PLLA‐PEG@PNIPAAm‐PDLA structure.magnified imageTEM image of the Au@PLLA‐PEG@PNIPAAm‐PDLA structure.

Nanomedicine for targeted drug delivery

“…chitosan, 37-41 dextran 42 ); 3) artificially synthesized polymers, especially aliphatic polyesters (i.e. poly lactide (PLA), 43,44 poly (lactide-r-glycolide) (PLGA), 45 and poly 3-caprolactone (PCL) 44 ), polyamide (i.e. poly L-lysine, 46 ) and others such as poly(methylmethacrylate) (PMMA) 47 and poly-(ethyl-2-cyanoacrylate) (PECA) 48 which are also being developed as nanoparticles for the same purpose.…”

“…A new class of temperature-programmed 'shell-in-shell' structures with two different copolymers synthesized by a modified-double-emulsion method (MDEM) was reported as an advanced generation nanocarrier. 43 Thermosensitive inner shells composed of poly(N-isopropylacrylamide-co-D,L-lactide) (PNIPAAm-PDLA) with a lower critical solution temperature (LCST) can be fabricated. This novel technique allows for the construction of a delivery vector with programmable release rates for any kind of hydrophilic chemotherapeutic agents into the polymeric nanocapsules.…”

Nanomaterials for Controlled Release of Anticancer Agents

“…A new class of temperature-programmed ''shell-in-shell'' structures with two different copolymers synthesized by a modified-double-emulsion method (MDEM) has been reported as an advanced generation nanovector [31]. In this approach, thermosensitive inner shells composed of poly(N-isopropylacrylamide-co-d,llactide) (PNIPAAm-PDLA) with a lower critical solution temperature (LCST) can be fabricated.…”

“…Especially, amine groups and cysteine residues in the proteins can be bound onto the surface of Au nanoparticles and can be stabilized electrostatically [71,75]. In parallel with this work, several groups have focused on the fabrication of Au nanoparticles-organic/inorganic hybrid structures with polyurethane (PU) [71], silica [78][79][80], polystyrene (PS) [81], and stimuli-sensitive polymers (SSPs) [31,62,82].…”

Electrospun micro/nanodevices for controlled biomolecule release

“…The collector was a grounded conductive substrate (a gold-coated glass) Polymer Shell and Liquid Core Materials: PDLA solution (solvent: chloroform) was used as the outer flow of the co-electrospray process. The hydrophobic property of PDLA satisfied a prerequisite for encapsulating aqueous liquids into a polymer shell using coelectrospray[80][81][82]. PDLA precursor solutions with different concentrations were prepared by mixing PDLA pellets (molecular weight 41000; Lactel Polymers) and chloroform (Sigma-Aldrich) in appropriate weight ratios.…”