The application of bioactive antioxidant compounds is limited because of
their instability. To overcome this drawback, different protection systems
including encapsulation have been developed. In this study, for the
stabilisation of bioactive compounds (carotenoids and phenolics) extracted
from tomato waste, encapsulation with carbohydrate (inulin and gum arabica)
and protein (soy protein and pea protein) wall materials by freeze drying
method was applied. Content of bioactive compounds and antioxidant
properties (determined by DPPH, reducing power and ?-carotene bleaching
assay) of encapsulates before and after in vitro digestion was investigated.
Also, in obtained encapsulates carotenoid stability during storage at
ambient (25 ? 5 ?C) light and dark conditions for four weeks was assessed.
The results indicated that release behavior of bioactive compounds and
antioxidant activity after digestion of encapsulates varied according to the
type of wall material. Protein carriers showed better ability to preserve
phenolic compounds through in vitro gastrointestinal digestion in comparison
to carbohydrate wall materials. Pea protein showed best carrier properties
for delivery of carotenoids, while differently to other used carriers, gum
arabica showed minor ability to release carotenoids after in vitro
gastrointestinal digestion. During storage higher content of carotenoids was
preserved in encapsulates prepared with carbohydrate carriers. All
encapsulates retained higher amount of carotenoids under dark conditions.
The results of this study showed that assessment of the behavior of
encapsulates during digestion and storage is necessary in order of selection
appropriate delivery system of bioactive compounds in powder form which can
be used as ingredient in functional food products.