Encapsulation of cells within silica matrixes: Towards a new advance in the conception of living hybrid materials

Meunier, Christophe; Dandoy, Philippe; Su, Bao‐Lian

doi:10.1016/j.jcis.2009.10.050

Cited by 125 publications

(98 citation statements)

References 116 publications

(123 reference statements)

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“…This procedure does not require preinfiltration of templating molecules [e.g., cationic polymers; (48)] or multistep layer by layer assembly and is distinct from other inorganic biotemplating strategies that simply coat external surfaces to produce hollow shells or low fidelity inverse structures following calcination (12,29). In contrast to the majority of studies describing cell encapsulation in silica (49) where the primary goal of maintaining cell viability necessitates reaction conditions near neutral pH and cells become physically entrapped within (nonconformal) gels, here the charge of silicic acid is essentially neutral (pH 3) (26,43) and thus hydrogen bonding and other noncovalent silica/molecular interactions govern deposition (35,43). To date, individual cellular/biomolecular components, peptides, proteins, lipid vesicles, polysaccharides, cytoskeletal filaments, etc.…”

Section: Resultsmentioning

confidence: 99%

Cellular complexity captured in durable silica biocomposites

Kaehr

Townson

Kalinich

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

103

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Tissue-derived cultured cells exhibit a remarkable range of morphological features in vitro, depending on phenotypic expression and environmental interactions. Translation of these cellular architectures into inorganic materials would provide routes to generate hierarchical nanomaterials with stabilized structures and functions. Here, we describe the fabrication of cell/silica composites (CSCs) and their conversion to silica replicas using mammalian cells as scaffolds to direct complex structure formation. Under mildly acidic solution conditions, silica deposition is restricted to the molecularly crowded cellular template. Inter-and intracellular heterogeneity from the nano-to macroscale is captured and dimensionally preserved in CSCs following drying and subjection to extreme temperatures allowing, for instance, size and shape preserving pyrolysis of cellular architectures to form conductive carbon replicas. The structural and behavioral malleability of the starting material (cultured cells) provides opportunities to develop robust and economical biocomposites with programmed structures and functions.sol-gel | biomineralization | biopreservation | frustule T he synthesis of inorganic materials with controlled and complex forms has been facilitated through discoveries such as vesicle, micelle, and liquid crystalline templating of silicates (1-3), which provided inspiration to explore a range of templating strategies based on self-assembled molecular precursors (4-8), colloids (9-11), and biological templates and vessels (12)(13)(14). A driving force for these efforts is the many complex inorganic structures found in nature. An oft-cited example is the hierarchical composites built by silica condensing microorganisms such as diatoms, which have generated substantial scientific interest for over a century (15). Diatoms display complex three-dimensional (3D) architectures with great structural control over nano-to millimeter length scales. However, despite some success toward elucidating mechanisms of diatom biomineralization, the in vitro synthesis of 3D diatom-like forms has remained elusive. Diatom silica has found numerous applications including as a chemical stabilizer, absorbent, filter medium, and fine abrasive, and the lack of synthetic analogues has facilitated recent investigations to employ diatom frustules as starting materials for shape-preserving chemical transformations into functional nanomaterials (16)(17)(18). Given the potential of this biosilica, it would be desirable to be able to wield control over the silica structure to achieve broader applicability (19); however, strategies to direct diatom morphology using chemical (20) and genetic approaches (21) has proven challenging. Therefore, an ability to generate cell frustules from more malleable templates such as mammalian cells would provide greater access to natural and engineered cell heterogeneity-both structure and function-to be exploited in the design of complex materials.To these ends, biomineralization by silica condensing microorganisms...

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Section: Resultsmentioning

confidence: 99%

Cellular complexity captured in durable silica biocomposites

Kaehr

Townson

Kalinich

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

103

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“…325 One of the first actions taken for this purpose was the removal of harmful by-products such as alcohol released during the process prior to the incorporation of microorganisms. 326 An alternative procedure is the so-called Biosil process, consisting in the use of common silicon alkoxide precursors in the vapour phase (chemical vapour deposition) which can react with surface-adsorbed H 2 O and exposed -OH.…”

Section: Silica and Silicate Biohybrids Incorporating Whole Cells Andmentioning

confidence: 99%

Hybrid and biohybrid silicate based materials: molecular vs. block-assembling bottom–up processes

2011

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“…These drawbacks can be overcome by the use of immobilized microorganisms in a matrix such as silica since it allows: (1) the protection of cells or microorganisms from harmful environments; (2) the control of their surrounding environment and of their concentration (Kato et al 2005;Meunier et al 2010). Therefore, this study aims to attempt a treatment strategy based on the silica-alginate fungi (Pleurotus sajor caju) biocomposites for removal of phenolic compounds in an OMW, thus decreasing its potential impact in the receiving waters and in the environment.…”

Section: Introductionmentioning

confidence: 99%

Removal of phenolic compounds in olive mill wastewater by silica–alginate–fungi biocomposites

Duarte

Justino

Panteleitchouk

et al. 2013

Int. J. Environ. Sci. Technol.

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This study aims to attempt a treatment strategy based on fungi immobilized on silica-alginate (biocomposites) for removal of phenolic compounds in olive oil mill wastewater (OMW), OMW supplemented (OMWS) with phenolic compounds and water supplemented (WS) with phenolic compounds, thus decreasing its potential impact in the receiving waters. Active (alive) or inactive (death by sterilization) Pleurotus sajor caju was encapsulated in alginate beads. Five beads containing active and inactive fungus were placed in a mold and filled with silica hydrogel (biocomposites). The biocomposites were added to batch reactors containing the OMW, OMWS and WS. The treatment of OMW, OMWS and WS by active and inactive biocomposites was performed throughout 28 days at 25°C. The efficiency of treatment was evaluated by measuring the removal of targeted organic compounds, chemical oxygen demand (COD) and relative absorbance ratio along the time. Active P. sajor caju biocomposites were able to remove 64.6-88.4 % of phenolic compounds from OMW and OMWS and 91.8-97.5 % in water. Furthermore, in the case of OMW there was also a removal of 30.0-38.1 % of fatty acids, 68.7 % of the sterol and 35 % of COD. The silica-alginate-fungi biocomposites showed a high removal of phenolic compounds from OMW and water. Furthermore, in the application of biocomposites to the treatment of OMW it was observed also a decrease on the concentration of fatty acids and sterols as well as a reduction on the COD.

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Encapsulation of cells within silica matrixes: Towards a new advance in the conception of living hybrid materials

Cited by 125 publications

References 116 publications

Cellular complexity captured in durable silica biocomposites

Cellular complexity captured in durable silica biocomposites

Hybrid and biohybrid silicate based materials: molecular vs. block-assembling bottom–up processes

Removal of phenolic compounds in olive mill wastewater by silica–alginate–fungi biocomposites

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