Encapsulation of clove oil in nanostructured lipid carriers from natural waxes: Preparation, characterization and in vitro evaluation of the cholinesterase enzymes

Meneses, Alessandra Cristina de; Marques, Elisa Balbi Pinto; Leimann, Fernanda Vitória; Gonçalves, Odinei Hess; Ineu, Rafael Porto; Araújo, Pedro Henrique Hermes de; Oliveira, Débora de; Sayer, Cláudia

doi:10.1016/j.colsurfa.2019.123879

Cited by 33 publications

(9 citation statements)

References 51 publications

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“…Other researchers reported similar results for NLCs containing the EO of Cuminun cyminum , with EE values ranging from 80 to 92% and using cocoa butter as a solid lipid as well [ 43 ]. It was demonstrated in other studies that NLCs are particularly useful for the encapsulation of lipophilic compounds, such as EO, with an EE normally greater than 70% [ 46 , 47 , 48 , 49 ]. The PEOC ranged from 1.37 (F13) to 3.01 (F10) mg PEO per gram of NLC.…”

Section: Resultsmentioning

confidence: 99%

Design of Nanostructured Lipid Carriers Containing Cymbopogon martinii (Palmarosa) Essential Oil against Aspergillus nomius

et al. 2021

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Palmarosa essential oil (PEO) is an alternative to synthetic fungicides to control the contamination by food-deteriorating fungi, such as Aspergillus nomius. Nonetheless, the low long-term stability and volatility hamper its utilization. Thus, this study aimed to develop nanostructured lipid carriers (NLCs) containing PEO to improve its stability and consequently prolong the activity against A. nomius. A mixture design was applied to find the best preparation conditions for antifungal activity. The characterization analyses included size measurements, zeta potential (ζ-potential), entrapment efficiency (EE), and antifungal activity (by inhibition of mycelial growth (IMG) and/or in situ test (pre-contaminated Brazil nuts) tests). The nanocarriers presented particle sizes smaller than 300 nm, homogeneous size distribution, ζ-potential of −25.19 to −41.81 mV, and EE between 73.6 and 100%. The formulations F5 and F10 showed the highest IMG value (98.75%). Based on the regression model, three optimized formulations (OFs) were tested for antifungal activity (IMG and in situ test), which showed 100% of inhibition and prevented the deterioration of Brazil nuts by A. nomius. The preliminary stability test showed the maintenance of antifungal activity and physicochemical characteristics for 90 days. These results suggest a promising system as a biofungicide against A. nomius.

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Section: Resultsmentioning

confidence: 99%

Design of Nanostructured Lipid Carriers Containing Cymbopogon martinii (Palmarosa) Essential Oil against Aspergillus nomius

et al. 2021

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“…Additionally, beeswax-crodamol nanoparticles showed a higher clove encapsulation efficiency (66 ± 1%–63 ± 0%) when compared with carnauba wax-crodamol ones (60 ± 2%–58 ± 0%): again, the presence of the liquid lipid is decisive. In both situations, the formulations showed no decrease in encapsulated clove percentages after 30 days storage, demonstrating the great suitability of these component in the formulation of NLC for clove delivery [ 199 ]. The wound-healing application of thymol-loaded NLC was recently investigated [ 200 ].…”

Section: Eos Encapsulation Strategies In Drug Delivery Systemsmentioning

confidence: 99%

Essential Oils: Pharmaceutical Applications and Encapsulation Strategies into Lipid-Based Delivery Systems

et al. 2021

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Essential oils are being studied for more than 60 years, but a growing interest has emerged in the recent decades due to a desire for a rediscovery of natural remedies. Essential oils are known for millennia and, already in prehistoric times, they were used for medicinal and ritual purposes due to their therapeutic properties. Using a variety of methods refined over the centuries, essential oils are extracted from plant raw materials: the choice of the extraction method is decisive, since it determines the type, quantity, and stereochemical structure of the essential oil molecules. To these components belong all properties that make essential oils so interesting for pharmaceutical uses; the most investigated ones are antioxidant, anti-inflammatory, antimicrobial, wound-healing, and anxiolytic activities. However, the main limitations to their use are their hydrophobicity, instability, high volatility, and risk of toxicity. A successful strategy to overcome these limitations is the encapsulation within delivery systems, which enable the increase of essential oils bioavailability and improve their chemical stability, while reducing their volatility and toxicity. Among all the suitable platforms, our review focused on the lipid-based ones, in particular micro- and nanoemulsions, liposomes, solid lipid nanoparticles, and nanostructured lipid carriers.

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“…They are often tested in vitro based on Ellman’s method using AChE extracted from electric eel and butyrylcholinesterase (BChE) from equine serum. Nonetheless, efforts were made to determine in vitro activity using the parasite Cotylophoron cotylophorum [ 34 ] and the fruit fly Drosophila melanogaster [ 35 ]. Ex vivo AChE activity was also measured, revealing no significant activity from S. cumini [ 36 ].…”

Section: Neuroprotective Agents From Syzygiummentioning

confidence: 99%

Roles of Syzygium in Anti-Cholinesterase, Anti-Diabetic, Anti-Inflammatory, and Antioxidant: From Alzheimer’s Perspective

Rawa

Mazlan

Ahmad

et al. 2022

Plants

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Alzheimer’s disease (AD) causes progressive memory loss and cognitive dysfunction. It is triggered by multifaceted burdens such as cholinergic toxicity, insulin resistance, neuroinflammation, and oxidative stress. Syzygium plants are ethnomedicinally used in treating inflammation, diabetes, as well as memory impairment. They are rich in antioxidant phenolic compounds, which can be multi-target neuroprotective agents against AD. This review attempts to review the pharmacological importance of the Syzygium genus in neuroprotection, focusing on anti-cholinesterase, anti-diabetic, anti-inflammatory, and antioxidant properties. Articles published in bibliographic databases within recent years relevant to neuroprotection were reviewed. About 10 species were examined for their anti-cholinesterase capacity. Most studies were conducted in the form of extracts rather than compounds. Syzygium aromaticum (particularly its essential oil and eugenol component) represents the most studied species owing to its economic significance in food and therapy. The molecular mechanisms of Syzygium species in neuroprotection include the inhibition of AChE to correct cholinergic transmission, suppression of pro-inflammatory mediators, oxidative stress markers, RIS production, enhancement of antioxidant enzymes, the restoration of brain ions homeostasis, the inhibition of microglial invasion, the modulation of ß-cell insulin release, the enhancement of lipid accumulation, glucose uptake, and adiponectin secretion via the activation of the insulin signaling pathway. Additional efforts are warranted to explore less studied species, including the Australian and Western Syzygium species. The effectiveness of the Syzygium genus in neuroprotective responses is markedly established, but further compound isolation, in silico, and clinical studies are demanded.

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Encapsulation of clove oil in nanostructured lipid carriers from natural waxes: Preparation, characterization and in vitro evaluation of the cholinesterase enzymes

Cited by 33 publications

References 51 publications

Design of Nanostructured Lipid Carriers Containing Cymbopogon martinii (Palmarosa) Essential Oil against Aspergillus nomius

Design of Nanostructured Lipid Carriers Containing Cymbopogon martinii (Palmarosa) Essential Oil against Aspergillus nomius

Essential Oils: Pharmaceutical Applications and Encapsulation Strategies into Lipid-Based Delivery Systems

Roles of Syzygium in Anti-Cholinesterase, Anti-Diabetic, Anti-Inflammatory, and Antioxidant: From Alzheimer’s Perspective

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