Encapsulation of Drug Nanoparticles in Self-Assembled Macromolecular Nanoshells

Zahr, Alisar S.; Villiers, Melgardt de; Pishko, Michael V.

doi:10.1021/la0478595

Cited by 134 publications

(109 citation statements)

References 45 publications

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“…Specifically, nanoparticles can be coated with polymeric nanoshells via layer-by-layer stepwise self-assembly [121]. The nanoshell provides a template upon which surface modifications can be made to create stealth or targeted nano-scale drug-delivery systems.…”

Section: Novel Systemsmentioning

confidence: 99%

Nanostructured materials for applications in drug delivery and tissue engineering

Goldberg

Langer

Jia

2007

Journal of Biomaterials Science, Polymer Edition

954

554

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Research in the areas of drug delivery and tissue engineering has witnessed tremendous progress in recent years due to their unlimited potential to improve human health. Meanwhile, the development of nanotechnology provides opportunities to characterize, manipulate and organize matter systematically at the nanometer scale. Biomaterials with nano-scale organizations have been used as controlled release reservoirs for drug delivery and artificial matrices for tissue engineering. Drug-delivery systems can be synthesized with controlled composition, shape, size and morphology. Their surface properties can be manipulated to increase solubility, immunocompatibility and cellular uptake. The limitations of current drug delivery systems include suboptimal bioavailability, limited effective targeting and potential cytotoxicity. Promising and versatile nano-scale drug-delivery systems include nanoparticles, nanocapsules, nanotubes, nanogels and dendrimers. They can be used to deliver both small-molecule drugs and various classes of biomacromolecules, such as peptides, proteins, plasmid DNA and synthetic oligodeoxynucleotides. Whereas traditional tissue-engineering scaffolds were based on hydrolytically degradable macroporous materials, current approaches emphasize the control over cell behaviors and tissue formation by nano-scale topography that closely mimics the natural extracellular matrix (ECM). The understanding that the natural ECM is a multifunctional nanocomposite motivated researchers to develop nanofibrous scaffolds through electrospinning or self-assembly. Nanocomposites containing nanocrystals have been shown to elicit active bone growth. Drug delivery and tissue engineering are closely related fields. In fact, tissue engineering can be viewed as a special case of drug delivery where the goal is to accomplish controlled delivery of mammalian cells. Controlled release of therapeutic factors in turn will enhance the efficacy of tissue engineering. From a materials point of view, both the drug-delivery vehicles and tissue-engineering scaffolds need to be biocompatible and biodegradable. The biological functions of encapsulated drugs and cells can be dramatically enhanced by designing biomaterials with controlled organizations at the nanometer scale. This review summarizes the most recent development in utilizing nanostructured materials for applications in drug delivery and tissue engineering.

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Section: Novel Systemsmentioning

confidence: 99%

Nanostructured materials for applications in drug delivery and tissue engineering

Goldberg

Langer

Jia

2007

Journal of Biomaterials Science, Polymer Edition

954

554

View full text Add to dashboard Cite

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“…28). Furthermore, the small size of these nanoparticles may allow them to escape uptake by mononuclear phagocytic cells and penetrate through the smallest capillary pores within the human vasculature (17,30).…”

Section: Introductionmentioning

confidence: 99%

Targeted Delivery of Gemcitabine to Pancreatic Adenocarcinoma Using Cetuximab as a Targeting Agent

et al. 2008

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One of the key challenges in anticancer therapy is the toxicity and poor bioavailability of the anticancer drugs. Nanotechnology can play a pivotal role by delivering drugs in a targeted fashion to the malignant cells that will reduce the systemic toxicity of the anticancer drug. In this report, we show a stepwise development of a nanoparticle-based targeted delivery system for in vitro and in vivo therapeutic application in pancreatic cancer. In the first part of the study, we have shown the fabrication and characterization of the delivery system containing gold nanoparticle as a delivery vehicle, cetuximab as a targeting agent, and gemcitabine as an anticancer drug for in vitro application. Nanoconjugate was first characterized physico-chemically. In vitro targeting efficacy, tested against three pancreatic cancer cell lines (PANC-1, AsPC-1, and MIA Paca2) with variable epidermal growth factor receptor (EGFR) expression, showed that gold uptake correlated with EGFR expression. In the second part, we showed the in vivo therapeutic efficacy of the targeted delivery system. Administration of this targeted delivery system resulted in significant inhibition of pancreatic tumor cell proliferation in vitro and orthotopic pancreatic tumor growth in vivo. Tumor progression was monitored noninvasively by measuring bioluminescence of the implanted tumor cells. Pharmacokinetic experiments along with the quantitation of gold both in vitro and in vivo further confirmed that the inhibition of tumor growth was due to targeted delivery. This strategy could be used as a generalized approach for the treatment of a variety of cancers characterized by overexpression of EGFR.

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“…The suitability of this synthetic PEs is still questioned as far as compatibility to bio-environment is concerned (16). Therefore, there could be two-tier approaches to mimic the system with bio-environment: (a) replacing synthetic with natural PEs and/or (b) modifying surface chemistry of developed system with bio-friendly agent using covalent coupling (17), hydrophobic interaction or adsorption by electrostatic interaction (18). It has been understood that replacing synthetic PEs with natural will undoubtedly lead to more biocompatible system.…”

Section: Introductionmentioning

confidence: 99%

Templated Ultrathin Polyelectrolyte Microreservoir for Delivery of Bovine Serum Albumin: Fabrication and Performance Evaluation

2011

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Abstract. The aim of the study was to develop ultrathin polyelectrolyte microreservoir (UPM) using two combinations of synthetic/synthetic (S/s; poly(allylamine hydrochloride) (PAH)/sodium poly (styrenesulfonate)) and synthetic/natural (S/n; PAH/sodium alginate) polyelectrolytes over spherical porous CaCO 3 core particles (CP) followed by core removal and to evaluate its biocompatibility and integrity of loaded model protein bovine serum albumin (BSA). A novel process for synthesis of CP was developed to obtain maximum yield of monodisperse vaterite (spherical) polymorph. The prepared UPM was characterized for surface morphology, layer-by-layer growth, pay load efficiency, integrity of BSA, as well as viability and cell adhesion using murine J 774 macrophages (Φ). In vitro release profile revealed that both S/s and S/n UPM were able to provide sufficient diffusion barrier to release protein at physiological pH. It has been observed that S/n UPM are fully biocompatible due to obvious reason of using natural polymer. In a separate experiment, the S/s UPM surface was modified with pluronic F-68 to tune biocompatibility which provides evidences for safety and tolerability of the S/s UPM as well. In nutshell, the proposed system could successfully be used for the delivery of proteins, and moreover, the system can be tailored to impart desired properties at any stage of layering especially in terms of drug release and to retain the integrity of proteins.

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Encapsulation of Drug Nanoparticles in Self-Assembled Macromolecular Nanoshells

Cited by 134 publications

References 45 publications

Nanostructured materials for applications in drug delivery and tissue engineering

Nanostructured materials for applications in drug delivery and tissue engineering

Targeted Delivery of Gemcitabine to Pancreatic Adenocarcinoma Using Cetuximab as a Targeting Agent

Templated Ultrathin Polyelectrolyte Microreservoir for Delivery of Bovine Serum Albumin: Fabrication and Performance Evaluation

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