Encapsulation of echinomycin in cyclodextrin inclusion complexes into liposomes: in vitro anti-proliferative and anti-invasive activity in glioblastoma

Abstract

Echinomycin, a DNA bis-intercalator peptide antibiotic, was complexed with γCD and loaded into PEGylated liposomes. The liposomes encapsulating echinomycin showed potent anti-proliferative and anti-invasive effect against U-87 MG glioblastoma cells.

Grafting of anti-nucleolin aptamer into preformed and remotely loaded liposomes through aptamer-cholesterol post-insertion

“…TEM micrographs were obtained using Morgagni (FEI, Netherlands) microscope operated at 60 kV. 29 2.6. Cellular assays 2.6.1.…”

Remote loading of curcumin-in-modified β-cyclodextrins into liposomes using a transmembrane pH gradient

“…Aer incubation, grids were washed with distilled water and dried at room temperature before imaging with Versa 3D (FEI, Netherlands) TEM operating system at an acceleration voltage of 30 kV. 11 2.8.5. Liposomes encapsulation efficiency (EE%).…”

“…Chemical shi (d) changes, either for the host or the guest, can verify due to the formation of inclusion complex in solution. 11,46 Fig. 7 represents the chemical structure of bCD and CRM with all hydrogen atoms that may be involved in complex formation.…”

“…TEM, the most frequently used imaging method for the evaluation of the structure of nanoparticles, 11,48 was employed to conrm the size and morphological characteristics of liposomes before and aer active loading. TEM observations (Fig.…”

Lipid nanostructures for targeting brain cancer

“…Moreover, cancer cells may develop resistance against most cytotoxic drugs during or shortly after therapy [27,28]. Recently, a new approach was applied to support and improve traditional treatments of tumors by utilizing nano-sized-carriers to overcome the side effects [29,30], enhance the low therapeutic efficacy, and show acceptable controlled release of the drug load [31]. Additionally, fabrication of nanocarriers with targeting moieties is possible, making them selectively bind to specific receptors overexpressed in cancer cells [32,33,34].…”