Encapsulation of in vitro-derived explants of olive (Olea europaea L. cv. Moraiolo)

Micheli, Maurizio; Hafiz, Ishfaq Ahmad; Standardi, A.

doi:10.1016/j.scienta.2007.04.001

Cited by 68 publications

(30 citation statements)

References 12 publications

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“…The reason behind this is the inhibition of the tissue respiration by the alginate medium or the loss of moisture content in the beads (Danso and The storage of encapsulated beads in cold temperature (4ºC) is most effective than room temperature in C. collinus plant. This was also reported in few plants such as Coelogyne breviscapa and Olea europaea (Mohanraj et al, 2009;Micheli et al, 2007). The regrowth efficiency was higher in encapsulated explants than non-encapsulated explants due to the addition of MS salts in the gelling matrix, which serves as an artificial nutrient to the explants.…”

Section: Low Temperature Storage and Shoot Re-growthsupporting

confidence: 62%

Encapsulation of shoot tips and nodal segments of Cleistanthus collinus for short term storage and germplasm exchange

Elangomathavan¹,

S²,

Hariharan³

et al. 2017

Int. J. Curr. Res. Biol. Med

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The encapsulation of nodal segments and shoot tips from in vitro raised Cleistanthus collinus (Roxb.) Benth and Hook (Euphorbiaceae) was performed using alginate (Gelling matrix) and calcium chloride (Complexing agent) for short term storage and germplasm preservation. The shoot re-growth frequency of encapsulated nodal segments and shoot tips after storage at 4ºC for different time periods (up to 8 weeks) were also assessed. Different combination and concentration of sodium alginate and calcium chloride was tested for the proper bead formation. The perfect synthetic seeds were obtained through a combination of 4% sodium alginate and 100 mM calcium chloride in half strength Murashige and Skoog medium with 3% sucrose. The maximum shoot re-growth response of encapsulated nodes (95%) and shoot tips (90%) were obtained in MS medium supplemented with 1.0 mg/l BAP after 6 weeks of culture. Encapsulated explants stored at 4ºC also produce maximum shoot regrowth frequency of 5.3 shoots / bead for nodal segments and 3.3 shoots / bead for shoot tips in subsequent weeks of transfer. There was higher response in nodal segments compared to shoot tip explants. The re-grown shoots from the encapsulated explants rooted best on half strength MS medium with 0.5 mg/l IAA. The regenerated plants were successfully hardened and acclimatized.

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Section: Low Temperature Storage and Shoot Re-growthsupporting

confidence: 62%

Encapsulation of shoot tips and nodal segments of Cleistanthus collinus for short term storage and germplasm exchange

Elangomathavan¹,

S²,

Hariharan³

et al. 2017

Int. J. Curr. Res. Biol. Med

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“…The most desirable feature of the encapsulated explants is their capability to retain viability in terms of regrowth and conversion abilities after encapsulation (Micheli et al, 2007;Adriani et al, 2000). In the present investigation, regrowth (defined as the number of shoots obtained per explant induced in 30, 60 and 90 days) and conversion (defined as the percentage of sown synthetic seeds that had performed both sprouting and rooting i.e., that had produced a complete plantlet) were the parameters used to measure the plantlet development.…”

Section: Characteristics Of Encapsulated Budsmentioning

confidence: 99%

Propagation through alginate encapsulation of axillary buds of Cannabis sativa L. — an important medicinal plant

Lata

Chandra

Khan

et al. 2009

Physiol Mol Biol Plants

104

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Cannabis sativa L. (Cannabaceae) is an important medicinal plant well known for its pharmacologic and therapeutic potency. Because of allogamous nature of this species, it is difficult to maintain its potency and efficacy if grown from the seeds. Therefore, chemical profile-based screening, selection of high yielding elite clones and their propagation using biotechnological tools is the most suitable way to maintain their genetic lines. In this regard, we report a simple and efficient method for the in vitro propagation of a screened and selected high yielding drug type variety of Cannabis sativa, MX-1 using synthetic seed technology. Axillary buds of Cannabis sativa isolated from aseptic multiple shoot cultures were successfully encapsulated in calcium alginate beads. The best gel complexation was achieved using 5 % sodium alginate with 50 mM CaCl 2 .2H 2 O. Regrowth and conversion after encapsulation was evaluated both under in vitro and in vivo conditions on different planting substrates. The addition of antimicrobial substance -Plant Preservative Mixture (PPM) had a positive effect on overall plantlet development. Encapsulated explants exhibited the best regrowth and conversion frequency on Murashige and Skoog medium supplemented with thidiazuron (TDZ 0.5 μM) and PPM (0.075 %) under in vitro conditions. Under in vivo conditions, 100 % conversion of encapsulated explants was obtained on 1:1 potting mix-fertilome with coco natural growth medium, moistened with full strength MS medium without TDZ, supplemented with 3 % sucrose and 0.5 % PPM. Plantlets regenerated from the encapsulated explants were hardened off and successfully transferred to the soil. These plants are selected to be used in mass cultivation for the production of biomass as a starting material for the isolation of THC as a bulk active pharmaceutical.

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“…Successful cases of synthetic seed production and plantlet regeneration have been reported for a wide range of plants including cereals, vegetables, fruits, ornamentals, medicinal plants and forest trees (Redenbaugh et al 1991;Castillo et al 1998;Ara et al 2000;Mandal et al 2000;Rai et al 2009). In most cases somatic embryos have been used in the encapsulation process, however, in recent years adequate efforts have also been made to explore the possibility of encapsulating non-embryogenic, in vitro-derived vegetative propagules such as axillary buds, shoot tips or nodal segments for synthetic seed production (Mathur et al 1989;Ganapathi et al 1992;Sharma et al 1994;Ara et al 2000;Mandal et al 2000;Rout et al 2001;Chand and Singh 2004;Naik and Chand 2006;Singh et al 2006aSingh et al , b, 2009Singh et al , 2010Faisal and Anis 2007;Micheli et al 2007;Rai et al 2008a, b;Lata et al 2009;Sundararaj et al 2010;Verma et al 2010). Encapsulation of vegetative propagules could be used for mass propagation of high value crops at a reasonable cost especially when somatic embryos are not available for encapsulation.…”

Section: Introductionmentioning

confidence: 99%

Alginate-encapsulation of shoot tips of jojoba [Simmondsia chinensis (Link) Schneider] for germplasm exchange and distribution

Kumar

Manoj

Singh

et al. 2010

Physiol Mol Biol Plants

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Shoot tips excised from in vitro proliferated shoots derived from nodal explants of jojoba [Simmondsia chinensis (Link) Schneider] were encapsulated in calcium alginate beads for germplasm exchange and distribution. A gelling matrix of 3 % sodium alginate and 100 mM calcium chloride was found most suitable for formation of ideal calcium alginate beads. Best response for shoot sprouting from encapsulated shoot tips was recorded on 0.8 % agarsolidified full-strength MS medium. Rooting was induced upon transfer of sprouted shoots to 0.8 % agar-solidified MS medium containing 1 mg l −1 IBA. About 70 % of encapsulated shoot tips were rooted and converted into plantlets. Plants regenerated from encapsulated shoot tips were acclimatized successfully. The present encapsulation approach could also be applied as an alternative method of propagation of desirable elite genotype of jojoba.

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Encapsulation of in vitro-derived explants of olive (Olea europaea L. cv. Moraiolo)

Cited by 68 publications

References 12 publications

Encapsulation of shoot tips and nodal segments of Cleistanthus collinus for short term storage and germplasm exchange

Encapsulation of shoot tips and nodal segments of Cleistanthus collinus for short term storage and germplasm exchange

Propagation through alginate encapsulation of axillary buds of Cannabis sativa L. — an important medicinal plant

Alginate-encapsulation of shoot tips of jojoba [Simmondsia chinensis (Link) Schneider] for germplasm exchange and distribution

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