Encapsulation of RNA Molecules in BSA Microspheres and Internalization into <i>Trypanosoma Brucei</i> Parasites and Human U2OS Cancer Cells

Shimanovich, Ulyana; Tkacz, Itai Dov; Eliaz, Dror; Cavaco‐Paulo, Artur; Michaeli, Shulamit; Gedanken, Aharon

doi:10.1002/adfm.201100963

Cited by 36 publications

(31 citation statements)

References 31 publications

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“…For example, acoustic emulsification of aqueous solutions in the presence of bovine serum albumin protein (BSA) and t-RNA yields encapsulation of the latter as an oil-core inside the BSA-shell (3D image in Figure 4C). 68 Freshly prepared RNA-encapsulating emulsions have a distinct red core (t-RNA) and a green shell (BSA) (image a in Figure 4C). The t-RNA core is delocalized closer to the walls of the sphere within 24-25 hours (image b in Figure 4C).…”

Section: Ultrasonic Emulsions With Proteinsmentioning

confidence: 99%

Ultrasonically treated liquid interfaces for progress in cleaning and separation processes

Radziuk

Möhwald

2016

Phys. Chem. Chem. Phys.

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Ultrasound and acoustic cavitation enable ergonomic and eco-friendly treatment of complex liquids with outstanding performance in cleaning, separation and recycling of resources. A key element of ultrasonic-based technology is the high speed of mixing by streams, flows and jets (or shock waves), which is accompanied by sonochemical reactions. Mass transfer across the phase boundary with a great variety of catalytic processes is substantially enhanced through acoustic emulsification. Encapsulation, separation and recovery of liquids are fast with high production yield if applied by ultrasound. Here we discuss the state of knowledge of these processes by ultrasound and acoustic cavitation from a perspective of a physico-chemical model in order to predict and control the outcome. We focus on the physical interpretation and quantification of ultrasonic parameters and properties of liquids to understand the chemistry of liquid/liquid interfaces in acoustic fields. The roles of thermodynamic enthalpy and entropy (incl. Laplace and osmotic pressure) in the context of sonochemical reactions (separation, catalysis, degradation, cross-linking, ion exchange and phase transfer) are outlined. The synergy of ultrasound and electric fields or continuous flow chemistry for cleaning and separation via emulsification is highlighted by specific strategies involving polymers and ultrasonic membranes.

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Section: Ultrasonic Emulsions With Proteinsmentioning

confidence: 99%

Ultrasonically treated liquid interfaces for progress in cleaning and separation processes

Radziuk

Möhwald

2016

Phys. Chem. Chem. Phys.

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“…[32] Ferritin is ac lass of ubiquitous iron storage and detoxification proteins within the cell. Ferritin nanocages,e specially recombinant human H-chain ferritin (rHuHF), have emerged as av ehicle for tumor imaging,a nd encapsulation and delivery of drugs [33][34][35][36][37][38] or bioactive nutrients [39] due to its exceptional characteristics,n amely biodegradability and functionalization versatility.M ore importantly,r HuHF receptor has been identified to be TfR-1w hich is highly expressed on human cancer cells,s ot his protein nanocage exhibits high selectivity for the cancer cells. In contrast, the L-subunit lacks such ferroxidase center.…”

mentioning

confidence: 99%

Conversion of the Native 24‐mer Ferritin Nanocage into Its Non‐Native 16‐mer Analogue by Insertion of Extra Amino Acid Residues

et al. 2016

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Protein assemblies with high symmetry are widely distributed in nature.M ost efforts so far have focused on repurposing these protein assemblies,astrategy that is ultimately limited by the structures available.T oo vercome this limitation, methods for fabricating novel self-assembling proteins have received intensive interest. Herein, by reengineering the key subunit interfaces of native 24-mer protein cage with octahedral symmetry through amino acid residues insertion, we fabricated a1 6-mer lenticular nanocage whose structure is unique among all knownprotein cages.This newly non-native protein can be used for encapsulation of bioactive compounds and exhibits high uptake efficiency by cancer cells. More importantly,the abovestrategy could be applied to other naturally occurring protein assemblies with high symmetry, leading to the generation of new proteins with unexplored functions.Livingsystemsutilizeproteinsasbuildingblockstoconstruct alarge variety of self-assembled nanoscale architectures.The assembly mechanism is governed by noncovalent interactions, such as hydrogen bonding,h ydrophobic effects and van der Waals interactions.Although separately these interactions are rather weak and transient, when am ultitude of noncovalent bonds act synergistically,s table protein architectures are shaped. [1][2][3] In any self-assembling protein architecture,s ubunit-subunit interactions (SSIs) play an important role.These noncovalent SSIs form large,h ighly complementary,l owenergy subunit-subunit interfaces.S uch interfaces spontaneously self-assemble and allow precise definition of the orientation of subunits relative to one another. Reported approaches to designing self-assembling proteins have satisfied this requirement in different ways,s uch as the use of engineered disulfide bonds, [4,5] electrostatic interactions, [6] chemical cross-links, [7] metal-mediated interactions, [8] ligandinduced association, [9] computational interface design, [10,11] or genetic fusion of multiple protein domains or fragments. [12,13] Theability to control SSIs would lead to the fabrication of the novel nanoscale protein materials with unexplored properties. However,t he task of rendering SSIs controllable is complicated by the fact that SSIs are mediated by weak, noncovalent interactions over large surfaces. [14,15] Protein cages are widely distributed in nature,w hich are constructed from as mall number of subunit building blocks by virtue of their subunit surfaces that self-assemble to highly cooperative,symmetrical structures.Incells,these cages have al arge variety of functions.F or example,v iral capsids are involved in nucleic acid storage and transport, [16] clathrin cages in endocytosis, [17] carboxysomes in CO 2 fixation, [18] Dps in DNAp rotection, [19] and ferritin cages in iron metabolism. [20] These protein cages have recently attracted considerable attention from researchers in the field of nanoscience and nanotechnology because they possess many useful properties such as high symmetry,s olubility and stability, monod...

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“…siRNA formulations such as microsphere encapsulation and nanoparticle complexation have been developed. [118] Although good transfection efficiencies and cellular responses are demonstrated, the transient effect limits their application. Electrospun nanofiber mediated siRNA delivery may serve as a potential alternative since the nanofiber can provide sustained long term delivery at the tumor site.…”

Section: Emerging Roles Of Electrospun Nanofibers In Cancer Researchmentioning

confidence: 99%

Emerging Roles of Electrospun Nanofibers in Cancer Research

Chen

Boda

Batra

et al. 2017

Adv Healthcare Materials

130

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This article reviews the recent progress of electrospun nanofibers in cancer research. It begins with a brief introduction to the emerging potential of electrospun nanofibers in cancer research. Next, a number of recent advances on the important features of electrospun nanofibers critical for cancer research are discussed including the incorporation of drugs, control of release kinetics, orientation and alignment of nanofibers, and the fabrication of 3D nanofiber scaffolds. This article further highlights the applications of electrospun nanofibers in several areas of cancer research including local chemotherapy, combinatorial therapy, cancer detection, cancer cell capture, regulation of cancer cell behavior, construction of in vitro 3D cancer model, and engineering of bone microenvironment for cancer metastasis. This progress report concludes with remarks on the challenges and future directions for design, fabrication, and application of electrospun nanofibers in cancer diagnostics and therapeutics.

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Encapsulation of RNA Molecules in BSA Microspheres and Internalization into Trypanosoma Brucei Parasites and Human U2OS Cancer Cells

Cited by 36 publications

References 31 publications

Ultrasonically treated liquid interfaces for progress in cleaning and separation processes

Ultrasonically treated liquid interfaces for progress in cleaning and separation processes

Conversion of the Native 24‐mer Ferritin Nanocage into Its Non‐Native 16‐mer Analogue by Insertion of Extra Amino Acid Residues

Emerging Roles of Electrospun Nanofibers in Cancer Research

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