Encapsulation of troglitazone and AVE0991 by gelation microspheres promotes epithelial transformation of adipose-derived stem cells

Tao, Ke; Bai, Xiaozhi; Zhang, Dongliang; Liu, Mengdong; Zhang, Yue; Han, Fangfang; Yang, Xuekang; Han, Juntao; Hu, Dahai

doi:10.1016/j.mcp.2020.101543

Cited by 3 publications

(1 citation statement)

References 36 publications

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“…Human ADSCs were isolated as previously described [28]. AD-SCs were cultured in 96-well plates at a density of 2 × 10 4 cells per well.…”

Section: Adsc Isolation and Cell Viabilitymentioning

confidence: 99%

A Composite of Hepatocyte Growth Factor- and 5α-Dihydrotestosterone-Gelatin Microspheres with Adipose-Derived Stem Cells Enhances Wound Healing

Tang¹,

Bai²,

Ji³

et al. 2022

Skin Pharmacol Physiol

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Introduction: Reconstructing sebaceous glands is one goal of functionally healing patients who have suffered severe burns, instead of the simple pursuit of wound closure. Effective regeneration of skin appendages remains a challenge in skin wound management and research. Objective: The aim of this study was to evaluate the differentiation of ADSC into sebaceous glands, and clarified the involvement of HGF and 5α-DHT in this process. Methods: This study used hepatocyte growth factor (HGF)– and 5α-dihydrotestosterone (5α-DHT)–gelatin microspheres to treat human adipose-derived stem cells (ADSCs) and investigated the reconstruction of sebaceous glands. HGF– and 5α-DHT–gelatin microspheres were constructed using microcapsule slow-release technology. Mice full-thickness skin-wound model was established to evaluate wound healing and hematoxylin-eosin staining was utilized to determine the skin structure. Results: In vitro analyses found that HGF– and 5α-DHT–gelatin microspheres promoted migration of and tube formation by ADSCs. Furthermore, AKT/ERK signaling, which is related to sebocyte and sweat gland epithelial cell growth, were activated after HGF and 5α-DHT treatment. An in vivo wound healing model demonstrated that ADSCs primed with amnion-loaded HGF– and 5α-DHT–gelatin microspheres promoted wound healing and increased sebaceous gland formation compared to the control group. Conclusions: This study confirms the efficacy of ADSCs treated with amnion and HGF– and 5α-DHT–gelatin microspheres in accelerating wound healing and effectively restoring sebaceous glands. This engineered tissue provides insight into and a novel therapeutic material for burns and full-thickness skin wounds.¬¬

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“…Human ADSCs were isolated as previously described [28]. AD-SCs were cultured in 96-well plates at a density of 2 × 10 4 cells per well.…”

Section: Adsc Isolation and Cell Viabilitymentioning

confidence: 99%

A Composite of Hepatocyte Growth Factor- and 5α-Dihydrotestosterone-Gelatin Microspheres with Adipose-Derived Stem Cells Enhances Wound Healing

Tang¹,

Bai²,

Ji³

et al. 2022

Skin Pharmacol Physiol

Self Cite

View full text Add to dashboard Cite

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Propofol suppresses adipose-derived stem cell progression via PI3K/AKT-Wnt signaling pathway

et al. 2022

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Adipose-derived stem cell (ADSC) transplantation has become a prospective way to treat cardiovascular diseases and skin traumas. Propofol, a short-acting intravenous anesthetic agent, plays an important role in the induction and maintenance of general anesthesia. In this study, we investigated the effects of propofol on ADSCs. The flow cytometry results showed that ADSCs were positive for CD29, CD44, and CD90 and negative for CD31, CD34, and CD45. The results of MTT and BrdU assays demonstrated that propofol impeded the proliferation of ADSCs. The cell scratch test showed that propofol had an inhibitory effect on the migration of ADSCs. Transwell assay showed that invasive ASDC counts decreased significantly after propofol treatment. Propofol also promoted ADSC apoptosis and arrested ADSCs in the G0/G1 phase. All these effects showed in a dose-dependent manner that the higher the concentration, the stronger the effect. Western blot analysis revealed decreased levels of FAK, PI3K, AKT, and GSK3β phosphorylation, while the phosphorylation of β-catenin increased after 48 h of treatment with propofol. The findings above indicated that the PI3K/AKT-Wnt pathways mediated propofol-inhibited ADSC proliferation, providing new insights into the propofol application in ADSCs.

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Advancement in Beneficial Effects of AVE 0991: A Brief Review

Deng

Ding

Peng

et al. 2024

MRMC

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AVE 0991, a non-peptide analogue of Angiotensin-(1-7) [Ang-(1-7)], is orally active and physiologically well tolerated. Several studies have demonstrated that AVE 0991 improves glucose and lipid metabolism, and contains anti-inflammatory, anti-apoptotic, anti-fibrosis, and anti-oxidant effects. Numerous preclinical studies have also reported that AVE 0991 appears to have beneficial effects on a variety of systemic diseases, including cardiovascular, liver, kidney, cancer, diabetes, and nervous system diseases. This study searched multiple literature databases, including PubMed, Web of Science, EMBASE, Google Scholar, Cochrane Library, and the ClinicalTrials.gov website from the establishment to October 2022, using AVE 0991 as a keyword. This literature search revealed that AVE 0991 could play different roles via various signaling pathways. However, the potential mechanisms of these effects need further elucidation. This review summarizes the benefits of AVE 0991 in several medical problems, including the COVID-19 pandemic. The paper also describes the underlying mechanisms of AVE 0991, giving in-depth insights and perspectives on the pharmaceutical value of AVE 0991 in drug discovery and development.

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Encapsulation of troglitazone and AVE0991 by gelation microspheres promotes epithelial transformation of adipose-derived stem cells

Cited by 3 publications

References 36 publications

A Composite of Hepatocyte Growth Factor- and 5α-Dihydrotestosterone-Gelatin Microspheres with Adipose-Derived Stem Cells Enhances Wound Healing

A Composite of Hepatocyte Growth Factor- and 5α-Dihydrotestosterone-Gelatin Microspheres with Adipose-Derived Stem Cells Enhances Wound Healing

Propofol suppresses adipose-derived stem cell progression via PI3K/AKT-Wnt signaling pathway

Advancement in Beneficial Effects of AVE 0991: A Brief Review

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