Encapsulation Preserves Antioxidant and Antidiabetic Activities of Cactus Acid Fruit Bioactive Compounds under Simulated Digestion Conditions

Abstract

Cactus acid fruit (Xoconostle) has been studied due its content of bioactive compounds. Traditional Mexican medicine attributes hypoglycemic, hypocholesterolemic, anti-inflammatory, antiulcerogenic and immunostimulant properties among others. The bioactive compounds contained in xoconostle have shown their ability to inhibit digestive enzymes such as α-amylase and α-glucosidase. Unfortunately, polyphenols and antioxidants in general are molecules susceptible to degradation due to storage conditions, (temperature, oxygen and light) or the gastrointestinal tract, which limits its activity and compromises its potential beneficial effect on health. The objectives of this work were to evaluate the stability, antioxidant and antidiabetic activity of encapsulated extract of xoconostle within double emulsions (water-in-oil-in-water) during storage conditions and simulated digestion. Total phenols, flavonoids, betalains, antioxidant activity, α-amylase and α-glucosidase inhibition were measured before and after the preparation of double emulsions and during the simulation of digestion. The ED40% (treatment with 40% of xoconostle extract) treatment showed the highest percentage of inhibition of α-glucosidase in all phases of digestion. The inhibitory activity of α-amylase and α-glucosidase related to antidiabetic activity was higher in microencapsulated extracts than the non-encapsulated extracts. These results confirm the viability of encapsulation systems based on double emulsions to encapsulate and protect natural antidiabetic compounds.

Microencapsulation of Syzygium zeylanicum (L.) DC . extract using spray drying: Effects of wall materials on physicochemical characteristics and biological activities of the microcapsules

“…In this study, the variation in the inhibitory effect against α-amylase and α-glucosidase enzymes was in agreement with the difference in the antioxidant activity of the of the encapsulated powders using different coating formulations. In consistence with the finding of this study, the literature on the encapsulation of phenolic-rich extracts also showed that the enzyme inhibitory activity of the microcapsules is highly correlated with their antioxidant activity which are attributed to the phenolic content in the samples (Medina-Pérez et al, 2020;Rambaran, 2020).…”

Impact of Different Drying Temperatures on In Vitro Antioxidant and Antidiabetic Activities and Phenolic Compounds of Wild Guava Leaves Collected in the Central Highland of Vietnam

“…Consistent with these findings, the literature on phenolic-rich extracts reveals that enzyme inhibitory activity is highly correlated with antioxidant activity, which has been attributed to the phenolic content in samples. 36,37…”

“…Consistent with these findings, the literature on phenolic-rich extracts reveals that enzyme inhibitory activity is highly correlated with antioxidant activity, which has been attributed to the phenolic content in samples. 36,37 Principal component analysis (PCA) was used to identify the relationship between the drying temperature, the correlation between extract bioactivities, and the contents of individual compounds. Figure 1 is a representation of the 6 drying treatments for all of the contents of the individual compound and extracts bioactivities.…”

Bioactive compounds and antibacterial activities in crystallized honey liquefied with ultrasound

“…The flavonoids were analyzed according the technique reported by Sancho et al [18] with some modifications Medina-Pérez et al [19] . One gram of honey was diluted (1:10) with methanol (Femont, MX), homogenized and centrifuged at 17,500 × g for 15 min at 4 °C.…”