Encapsulation, slime production and surface hydrophobicity of coagulase-negative staphylococci

Hogt, A.H.; Dankert, J.; Feijén, Jan

doi:10.1111/j.1574-6968.1983.tb00480.x

Cited by 79 publications

(17 citation statements)

References 16 publications

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“…In almost all studies [with the exception of some encapsulated staphylococci, according to Hogt et al (1983a)] the presence of bacterial capsule or slime has been shown to decrease surface hydrophobicity. For example, enzymatic removal of the capsule of Acinetobacter calcoaceticus BD4 led to an increase in adhesion to hexadecane from 0 to 100% (E. .…”

Section: Role Of Capsulementioning

confidence: 99%

Hydrophobic Interactions: Role in Bacterial Adhesion

Rosenberg

Kjelleberg

1986

Advances in Microbial Ecology

300

176

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Section: Role Of Capsulementioning

confidence: 99%

Hydrophobic Interactions: Role in Bacterial Adhesion

Rosenberg

Kjelleberg

1986

Advances in Microbial Ecology

300

176

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“…Since the role of hydrophobic moieties in bacterial adherence is well documented [10,11], the effect of antimicrobial agents on cell surface hydrophobicity was also investigated.…”

Section: Introductionmentioning

confidence: 99%

Reduction of Mucoid <i>Staphylococcus epidermidis</i> Adherence to Intraocular Lenses by Selected Antimicrobial Agents

Kadry¹,

Tawfik²,

El-Asrar

et al. 1999

Chemotherapy

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The effect of low concentrations of various antimicrobial agents on the adherence of mucoid Staphylococcus epidermidis to intraocular lenses was investigated. Adherent growth of S. epidermidis on to polymethylmethacrylate lenses was greatly diminished by ciprofloxacin and clindamycin (>75%), followed by ceftazidime (≈50%) and less affected by gentamicin and vancomycin (<25%) compared to the adherent growth of control untreated cells. The reduction in adherence growth was concentration-dependent and found to be due to the inhibition of slime production since no growth inhibition was observed at the concentrations used. Cell surface hydrophobicity was markedly reduced in parallel to the inhibition of adherence as compared to the control cells. The differential effect of antibiotics on adherence, regardless of their antimicrobial activity, may have a clinical significance in reducing the incidence of postoperative endophthalmitis and intraocular inflammation due to the reduction of cell adherence.

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“…epidermidis including production of extracellular polysaccharide (Evans et al 1994). Bacterial cell surface hydrophobicity is an important determinant of adherence (Hogt et al 1983) and staphylococcal cell surface hydrophobicity is also influenced by growth conditions (Ljungh and Wadstrom 1995). Many biomedical devices are exposed to conditions of shear stress and most studies of bacterial adhesion do not take account of conditions of flow (Busscher and Van der Mei 1995).…”

Section: Introductionmentioning

confidence: 99%

Use of a modified Robbins device to directly compare the adhesion ofStaphylococcus epidermidisRP62A to surfaces

Linton¹,

Sherriff

Millar³

1999

Journal of Applied Microbiology

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C .J . L I NT ON , A. SH E RR IF F AN D M . R. MI L LA R. 1999.Staphylococcus epidermidis is a frequent cause of infection associated with the use of biomedical devices. Flow cell studies of the interaction between bacteria and surfaces do not generally allow direct comparison of different materials using the same bacterial suspension. The use of a modified Robbins Device (MRD) to compare the adhesion to different surfaces of Staph. epidermidis RP62A grown in continuous culture was investigated. Adhesion to glass was compared with siliconized glass, plasma-conditioned glass, titanium, stainless steel and Teflon. Attachment to siliconized glass was also compared with glass under differing ionic strength, and divalent cation concentrations. Both the differences in numbers adhering and changes in adhesion (slope) through the MRD were compared. There was a trend towards higher numbers adhering to the discs at the in-flow end of the MRD than at the outflow end, probably reflecting depletion of adherent bacteria in the interacting stream. Adhesion of Staph. epidermidis RP62A to siliconized glass and Teflon was reduced when compared to glass with increasing flow rates. Adhesion to stainless steel was not affected by flow rate and titanium gave a different slope of adhesion through the MRD when compared with glass, suggesting an interaction with different sub-populations within the interacting stream. Differences between siliconized glass and glass at flow rates of 300 ml h −1 were abolished by the addition of calcium or EDTA and reduced by the addition of magnesium. Increasing ionic strength reduced the statistical significance of the differences between glass and siliconized glass. Pre-conditioning of glass with pooled human plasma reduced adhesion compared with untreated glass and again gave a different slope to glass. The MRD linked to a chemostat can be used to compare directly bacterial adhesion to potential biomaterials. Variable depletion of the interacting stream should be taken into account in the interpretation of results. Divalent cation concentration, substrate properties and flow rate were important determinants of the comparative adhesion of Staph. epidermidis RP62A to surfaces.

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Encapsulation, slime production and surface hydrophobicity of coagulase-negative staphylococci

Cited by 79 publications

References 16 publications

Hydrophobic Interactions: Role in Bacterial Adhesion

Hydrophobic Interactions: Role in Bacterial Adhesion

Reduction of Mucoid <i>Staphylococcus epidermidis</i> Adherence to Intraocular Lenses by Selected Antimicrobial Agents

Use of a modified Robbins device to directly compare the adhesion ofStaphylococcus epidermidisRP62A to surfaces

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