End-Column Electrochemical Detection for Capillary Electrophoresis

Matysik, Frank‐Michael

doi:10.1002/1521-4109(200011)12:17<1349::aid-elan1349>3.0.co;2-9

Cited by 32 publications

(5 citation statements)

References 106 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A few years later Ewing's group also introduced the first hyphenation of CE with AD without an electrical field decoupler termed end-column AD [8]. For electroactive analytes, AD is considered as a very sensitive detection method [9][10][11]. In combination with a nonaqueous background electrolyte (BGE), the long-term stability of AD is excellent, allowing the quantification utilizing external calibration or standard addition method [12,13].…”

Section: Introductionmentioning

confidence: 99%

Combining amperometry and mass spectrometry as a dual detection approach for capillary electrophoresis

2022

View full text Add to dashboard Cite

Dual detection concepts (DDCs) are becoming more and more popular in analytical chemistry. In this work, we describe a novel DDC for capillary electrophoresis (CE) consisting of an amperometric detector (AD) and a mass spectrometer (MS). This detector combination has a good complementarity as the AD exhibits high sensitivity, whereas the MS provides excellent selectivity. Both detectors are based on a destructive detection principle, making a serial detector arrangement impossible. Thus, for the realization of the DDC, the CE flow was divided into two parts with a flow splitter. The DDC was characterized in a proof-of-concept study with ferrocene derivates and a nonaqueous background electrolyte. We could show that splitting the CE flow was a suitable method for the instrumental realization of the DDC consisting of two destructive detectors. By lowering the height of the AD compared to the MS, it was possible to synchronize the detector responses. Additionally, for the chosen model system, we confirmed that the AD was much more reproducible and had lower limits of detection (LODs) than the MS. The LODs were identical for the DDC and the single-detection arrangements, indicating no sensitivity decrease due to the CE flow splitting. The DDC was successfully applied to determine the drug and doping agent trimetazidine.

show abstract

Section: Introductionmentioning

confidence: 99%

Combining amperometry and mass spectrometry as a dual detection approach for capillary electrophoresis

2022

View full text Add to dashboard Cite

show abstract

“…Electrochemical detection for capillary separations is particularly attractive because the electrochemical detection can be conducted on low volumes with little loss of sensitivity [6,7]. The large electric field from the electrophoresis separation, however, interferes with the electrochemical detection and potentially limits this method [3,8,9]. To avoid this interference, the electrophoretic field is decoupled from the field associated with the electrochemical detector.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Electrode Alignment for Electrochemical Detection in Capillary Electrophoresis Using a Scanning Electrochemical Microscope

2005

View full text Add to dashboard Cite

Electrochemical detection in capillary electrophoresis requires decoupling the voltage applied to the working electrode from the separation voltage applied across the capillary. End-capillary electrochemical detection achieves this by placing the electrode just outside the ground end of the separation capillary. Obtaining adequate signal-tonoise in this arrangement requires using small inner diameter capillaries. Decreasing the inner diameter of the separation capillary, however, increases the difficulty of aligning the microelectrode with the open end of the capillary. Using scanning electrochemical microscopy (SECM), the position of the capillary opening is determined while electroactive material is continuously emerging from the end of the capillary. The SECM instrument is then used to place the electrode at the position of maximum current for subsequent separations. Subsequent measurements found that the best signal-to-noise is obtained when the detection electrode is placed directly opposite the capillary opening and just outside of the capillary opening. When the electrode is further above the opening (but still opposite the capillary opening), the signal-to-noise does not dramatically decrease until the electrode is more than 30 mm above the 10 mm inner-diameter capillary.

show abstract

“…The size of the electrodes is another important parameter in CE with electrochemical detection [22][23][24]. It is known that the best performance, is obtained when the working electrode diameter is higher than the capillary column inner diameter.…”

Section: Resultsmentioning

confidence: 99%

Capillary electrophoresis of neurotransmitters with amperometric detection at melanin-type polymer-modified carbon electrodes

Chicharro

Sánchez

Zapardiel

et al. 2004

Analytica Chimica Acta

View full text Add to dashboard Cite

End-Column Electrochemical Detection for Capillary Electrophoresis

Cited by 32 publications

References 106 publications

Combining amperometry and mass spectrometry as a dual detection approach for capillary electrophoresis

Combining amperometry and mass spectrometry as a dual detection approach for capillary electrophoresis

Optimization of Electrode Alignment for Electrochemical Detection in Capillary Electrophoresis Using a Scanning Electrochemical Microscope

Capillary electrophoresis of neurotransmitters with amperometric detection at melanin-type polymer-modified carbon electrodes

Contact Info

Product

Resources

About