End Group and Sedimentation Data on Fragmented High Molecular Weight Ribonucleates

Lane, B. G.; Diemer, J.; Blashko, Carl A.

doi:10.1139/y63-219

Cited by 8 publications

(9 citation statements)

References 16 publications

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“…Evidence has been presented in previous publications (12,13) which showed that the chain ends detected as rlucleosides and diphosphonucleosides after Can. J. Biochem.…”

Section: Resultsmentioning

confidence: 71%

“…The end groups detected in IS S + 2S S ribonucleates by hydrolysis with purified snake venom phosphodiesterase are probably formed, in large part, as the result of spontaneous hj~droxyl ion catal~yzed cleavages during the analysis itself. The lonr recoverJr of nucleoside diphosphates in phosphodiesterase digests could reflect 21 partial destruction and poor recovery of these compounds in such digests (13,13), and it is therefore nlandatory to base estimates of minimal chain length on analysis for the type of chain end which is linked by a phosphodiester bridge to its 3' position. The latter chain termini are not subject to an!-known destruction after release by alkali or phosphodiesterase hydrolysis, and appear cluantitatively as nucleosides in phosphodiesterase digests if they do not bear pliospliomonoester groups and appear quantitatively as nucleoside diphosphates in alkali hyclrolj~zates if they bear a phosphornonoester group at the 5' position.…”

Section: Resultsmentioning

confidence: 99%

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The Alkali-Stable Dinucleotide Sequences in 18 s + 28 s Ribonucleates From Wheat Germ

Singh¹,

Lane²

1964

Can. J. Biochem.

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The 2'-0-i~lethyl substitution of ribose residues of ribonucleate chains can give rise to 16 alkali-stable dinucleotides having the general structure NxpNp in which N is any of the four major ribonucleosides and Nx is the 2'-0-methj.1 derivative of any of the four major ribonucleosides. Quantitative data are presented which show that all of these 16 alkali-stable dinucleotides occur in characteristic a~~~o u n t s in alkali hydrolyzates of the 18 S + 28 S ribonucleates from wheat germ. 'The alltali-stable dinucleotitles cumulatively account for 3 mole% of the total nucleotides of the 18 S + 28 S wheat germ ribonucleates. The relative amounts of the four 2'-0-methyl ribosides responsible for the alkali stabilit). of the tlinucleotitlcs have been shown to be distinctly different froin the relative an~ounts of the four corresponding normal ribosides which participate in either the allcali-stable dinucleotide sequences or in the ribonucleate chains as a whole. Quantitati\-e end group data show that the alkali-stable dinucleotide sequences occur internally in high molecular weight ribonucleate chains. Preliminary data are presented 011 the occurrence of alkali-stable trinucleotides in alkali hydrolyzates of the 18 S + 28 S wheat germ ribonucleates.

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“…Evidence has been presented in previous publications (12,13) which showed that the chain ends detected as rlucleosides and diphosphonucleosides after Can. J. Biochem.…”

Section: Resultsmentioning

confidence: 71%

Section: Resultsmentioning

confidence: 99%

The Alkali-Stable Dinucleotide Sequences in 18 s + 28 s Ribonucleates From Wheat Germ

Singh¹,

Lane²

1964

Can. J. Biochem.

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“…Isolation of 5-Carboxymethyluridine 5'-Phosphate from tRNA. To check the possibility that 5-carboxymethyluridine might be present as the free carboxylic acid in tRNA, wheat embryo tRNA was hydrolyzed with purified snake venom phosphodiesterase under conditions that achieve complete hydrolysis of the RNA (Lane et al, 1963;Hudson et al, 1965). The hydrolysis products were fractionated on DEAE-cellulose according to net charge at pH 7.8, and the fraction which eluted after the 5'-nucleotides was examined to determine if it contained any 5-carboxymethyluridine 5'-phosphate.…”

Section: Resultsmentioning

confidence: 99%

“…The developing solvents were 9 5 z ethyl alcohol-water (4:1, v/v) (system 1) and saturated ammonium sulfate-2-propanol (40: 1, v/v) (system 2); they were used in conjunction with Whatman No. 1 chromatography paper which had been impregnated with ammonium sulfate (Lane, 1963).…”

Section: Methodsmentioning

confidence: 99%

5-Carboxymethyluridine, a novel nucleoside derived from yeast and wheat embryo transfer ribonucleates

Gray¹,

Lane²

1968

Biochemistry

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A carboxyl-containing nucleoside, 5-carboxymethyluridine, has been isolated from the transfer ribonucleates of bakers' yeast, brewers' yeast, and wheat embryo. The 2'-and 3'-nucleotides of 5-carboxymethyluridine were isolated from alkali hydrolysates of the purified transfer ribonucleate preparations. A phosphodiesterase hydrolysate of wheat embryo transfer ribonucleates was examined for the presence of 5-carboxymethyluridine 5'-phosphate, but none could be detected. However, after alkali treatment of the 5'-nucleotides produced by the action of phosphodiesterase on wheat embryo transfer ribonucleates, a compound having the properties of the 5'-nucleotide of 5-carboxyinethyluridine was isolated. This observation suggests A lkali hydrolysis of tRNA or rRNA releases nucleosides (N) and nucleoside 2'(3 '),5 '-bisphosphates (pNp) from chain termini, and nucleoside 2'(3 ')-monophosphates (Np) and alkali-stable dinucleotides (NmpNp)' from internal positions of the ribonucleate chains. By ion-exchange chromatography on DEAEcellulose columns, using urea-containing eluents (Tomlinson and Tener, 1963), these four types of product can be resolved from one another according to their different net charges at pH 7.8 (N, 0; Np, -2; NmpNp, -3; and pNp, -4). Stepwise elution with urea-containing eluents of increasing ionic strength cleanly resolves the

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“…The first problem can be solved by methods that give nuclease-free preparations such as that ofCox & Arnstein (1963). For terminalgroup analysis non-radioactive techniques (Lane, Diemer & Blashko, 1963) require amounts of about 50pug. of the terminal nucleoside, thus requiring 150mg.…”

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confidence: 99%

Terminal-Sequence Studies of High-Molecular-Weight Ribonucleic. The Reaction of Periodate-Oxidized Ribonucleosides, 5′-Ribonucleotides and Ribonucleic Acid With Isoniazid

Hunt¹

1965

Biochemical Journal

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1. The reaction products of isoniazid with periodate-oxidized ribonucleosides and 5'-ribonucleotides have been characterized as the monohydrazones. 2. The stability, chromatographic and electrophoretic properties of the hydrazones are described. 3. (3)H-labelled isoniazid was shown to react with the 5'-linked terminal adenosine and cytidine groups of periodate-oxidized Escherichia coli transfer RNA. One mole of isoniazid reacts with 27x10(3)g. of the transfer RNA. 4. One mole of (3)H-labelled isoniazid reacts with approx. 10(6)g. of rabbit-reticulocyte ribosomal RNA. After fractionation of the RNA into its two components and treating the fractionated material with pancreatic ribonuclease and ribonuclease T(1) evidence is presented for the existence of two 5'-linked terminal sequences in the 30s fraction and only one sequence in the 17s fraction. 5. The application of this method to determining terminal sequences of high-molecular-weight RNA is discussed.

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End Group and Sedimentation Data on Fragmented High Molecular Weight Ribonucleates

Cited by 8 publications

References 16 publications

The Alkali-Stable Dinucleotide Sequences in 18 s + 28 s Ribonucleates From Wheat Germ

The Alkali-Stable Dinucleotide Sequences in 18 s + 28 s Ribonucleates From Wheat Germ

5-Carboxymethyluridine, a novel nucleoside derived from yeast and wheat embryo transfer ribonucleates

Terminal-Sequence Studies of High-Molecular-Weight Ribonucleic. The Reaction of Periodate-Oxidized Ribonucleosides, 5′-Ribonucleotides and Ribonucleic Acid With Isoniazid

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