In aging societies, nursing homes that provide 24-hour functional support to frail elderly residents (1), who require assistance due to diminished capacity, play an important role in end-of-life (EOL) care. In Japan, EOL care in nursing homes has gradually increased over the past decade (2-4). However, approximately 30% of facilities continue to transfer residents to hospitals for EOL care (2,4). In the remaining 70% of facilities, approximately 40% of the residents receive EOL care and ultimately die in hospitals rather than in nursing homes (2,4). Thus, residents may be transferred to hospitals against their wishes for EOL care. Multidisciplinary care is a requirement of EOL care in nursing homes. However, providing EOL care overnight is challenging. Nurses in nearly all nursing homes work on-call shifts during the evenings (5). Professional caregivers in nursing homes usually perform EOL care without assistance during the nighttime (6). Thus, the professional caregiver's role may be practically the most important. Professional caregivers who do not have sufficient experience with EOL care have fears about EOL care (7). Thus, multidisciplinary care is essential to prevent unnecessary anxiety in nighttime professional caregivers who are inexperienced with EOL care. EOL care conferences were introduced in the Japanese guidelines for EOL care in nursing homes (8), which state that such conferences promote Summary End-of-life (EOL) care conferences have an important role in promoting EOL care in nursing homes. However, the details of the conferences remain poorly understood. A Japanese prefecture-wide survey was conducted to investigate the factors involved in such conferences that contribute to an increase in the amount of EOL care. One hundred fiftythree nursing homes performed the conferences. The outcome was the amount of EOL care provided in nursing homes after adjusting for the facility beds in 2014. We investigated the factors of staff experience with EOL care, frequency of the conferences, years the conferences were conducted, review conferences after EOL care, and professional participants in the conferences. The multivariate analysis revealed significant associations between EOL care in nursing homes and nurses' experience with EOL care (adjusted β coefficient 2.9, 95% confidence interval (CI) 0.52 ~ 5.22, p = 0.017), more than 5 years of continuous conferences (adjusted β coefficient 3.8, 95% CI 0.46 ~ 7.05, p = 0.026), and family participation (adjusted β coefficient-4.0, 95% CI-7.5 ~-0.48, p = 0.026). In conclusion, the continuation of conferences and enrollment of the nurse with experience in EOL care may promote EOL care in nursing homes, while family enrollment in conferences may decrease EOL care in nursing homes. EOL care conferences in nursing homes should be continuously performed by staff, with an experienced nurse undertaking the task of information sharing before discussing EOL care with the patients' families.