End-to-end gene fusion of cellulase and hemicellulases for valorization of fruit peels: structural and techno-functional properties of eco-friendly extracted pectin

Monica, P; Prabha, Neelam; Kapoor, M.

doi:10.1007/s13399-022-03553-3

Cited by 2 publications

(2 citation statements)

References 62 publications

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“…Degree of esterification (DE) is defined as the amount of galacturonic acid units that are methyl-esterified at C6. DE values of less than 50% are classified as low methoxyl pectin, while DE values higher than 50% are considered high methoxyl pectin (EZZATI et al, 2020;MONICA;PRABHA;KAPOOR, 2022).…”

Section: Degree Of Esterification (De) and Degree Of Methylation Cont...mentioning

confidence: 99%

Phenolic compounds and pectin-rich extracts recovered from peach pomace by sequential pressurized liquid extractions

Rudke,

Germano

et al. 2024

Preprint

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Peach pomace is a by-product from the peach industrial processing, rich in high-value bioactive compounds and pectin. This study aimed to conduct a sequential pressurized liquid extraction (PLE) for the fractionation of peach pomace, recovering phenolics and pectin extracts. These separated fractions were evaluated, and novel functional properties were determined. The alternative high-pressure method (PLE) was compared to low-pressure sequential extractions conducted by Soxhlet (SOX) and heat-stirred extraction (HSE), as standard procedures. The results indicate that PLE at 40 °C provided highest phenolics yield (10.31 mgGAE.g-1), exhibiting antioxidant potential, by DPPH, ABTS and FRAP, of 20.01, 37.57 and 155.15 µmol TE. g-1, respectively. The pectin yield was 20% by HSE, while PLE achieved maximum yield of 14%. Nevertheless, the quality of the pectin fraction indicated higher content of galacturonic acid from the PLE pectin (47 % with 31 % of degree of esterification), compared to the HSE sample (10.90 % galacturonic acid with 22.15 % degree of esterification). Besides, the functional properties of the pectin fraction show high water solubility (96.47%) without significant difference between PLE and HSE samples. The PLE-pectin exhibited low water-holding capacity (0.03 g water/g) but higher oil-holding capacity than HSE-pectin. Both samples showed good emulsification and foaming ability and stability, with a higher value for the HSE sample. The present results suggest peach pomace as a promising by-product for the recovery of phenolic-rich and pectin-rich fractions. This study introduces innovations for the peach pomace fractionation and sheds light on the techno-functional properties of the pectin-rich fraction, which have proven promising attributes for future applications in food formulations.

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Section: Degree Of Esterification (De) and Degree Of Methylation Cont...mentioning

confidence: 99%

Phenolic compounds and pectin-rich extracts recovered from peach pomace by sequential pressurized liquid extractions

Rudke,

Germano

et al. 2024

Preprint

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“…Pectin-rich fruit peels are a further potential source of valorisable monosaccharides such as Ara and Gal (Yapo et al 2007;Li et al 2016;Lachos-Perez et al 2020;Jang et al 2022;Monica et al 2022;Gao et al 2023;Zhou and Huang 2023). The peels of citrus and other common fruits are major sidestreams of the food industry whose disposal by dumping in the environment is linked to severe ecological impacts (Jang et al 2022).…”

Section: Introductionmentioning

confidence: 99%

Isolation of arabinose and galactose from industrial sidestreams in high yield and purity

Widsten,

West,

Vaidya

et al. 2023

BioRes

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Monosaccharides such as L-arabinose (Ara) and D-tagatose (derived from D-galactose, Gal) are low-calorie sweeteners associated with improved glycaemic and insulin control compared to disaccharides such as sucrose. However, alternative sources and better sugar-sugar separation methods are needed to improve the sustainability and economics of their production. Here, these sugars were obtained from purified and ultrafiltered compression screw pressate (CSP) of thermo-mechanical pulping of softwood (Pinus radiata) and orange peels (OPs). Ba-substituted zeolite X (BaX) molecular sieves showed superior separation performance of Ara from other sugars compared to conventional Ca-form ion exchange resin. To facilitate subsequent separation of sugars, OP hydrolysates were fermented to leave just Ara and Gal, while OP pectin was hydrolysed to generate a Gal-rich mixture. Overall, BaX has good potential for separating Ara from Ara-rich hydrolysates containing several different sugars. It is also suited for separating Ara and another monosaccharide such as Gal or Xyl in the absence of other sugars.

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End-to-end gene fusion of cellulase and hemicellulases for valorization of fruit peels: structural and techno-functional properties of eco-friendly extracted pectin

Cited by 2 publications

References 62 publications

Phenolic compounds and pectin-rich extracts recovered from peach pomace by sequential pressurized liquid extractions

Phenolic compounds and pectin-rich extracts recovered from peach pomace by sequential pressurized liquid extractions

Isolation of arabinose and galactose from industrial sidestreams in high yield and purity

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