End-to-side neurorrhaphy using an electrospun PCL/collagen nerve conduit for complex peripheral motor nerve regeneration

Lee, Bu Kyu; Ju, Young Min; Cho, Jae-Gu; Jackson, John D.; Lee, Sang Jin; Atala, Anthony; Yoo, James J.

doi:10.1016/j.biomaterials.2012.09.008

Cited by 89 publications

(56 citation statements)

References 47 publications

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“…Although many natural (e.g. collagen) (Kemp et al, 2009;Li et al, 1991) and synthetic (Polycaprolactone (PCL), chitosan, polyglycolic acid (PGA)) (Jansen et al, 2004;Lee et al, 2012;Meek and Jansen, 2009;Yao et al, 2009) conduits have been developed in an attempt to replace the requirement for autografts, functional recovery that matches autograft levels is seldom observed (Kehoe et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Decellularized grafts with axially aligned channels for peripheral nerve regeneration

Sridharan

Reilly

Buckley

2015

Journal of the Mechanical Behavior of Biomedical Materials

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At least 2 million people worldwide suffer annually from peripheral nerve injuries (PNI), with estimated costs of $7 billion incurred due to paralysis alone. The current "gold" standard for treatment of PNI is the autograft, which poses disadvantages such as high fiscal cost, possible loss of sensation at donor site and the requirement of two surgeries. Allografts are viable alternatives; however, intensive immunosuppressive treatments are often necessary to prevent host rejection. For this reason, significant efforts have been made to remove cellular material from allografts. These decellularized nerve grafts perform better than other clinically available grafts but not as well as autografts; therefore, current research on these grafts includes the incorporation of additional components such as growth factors and cells to provide chemical guidance to regenerating axons. However, effective cellular and axonal penetration is not achieved due to the small pore size (5-10μm) of the decellularized grafts.The overall objective of this study was to induce axially aligned channels in decellularized nerve grafts to facilitate enhanced cell penetration. The specific aims of this study were to optimize a decellularization method to enhance cellular removal, to induce axially aligned pore formation in decellularized grafts through a novel unidirectional freeze drying method, to study the bulk mechanical properties of these modified decellularized grafts and to assess cell penetration into these grafts. To this end we modified an existing decellularization protocol to improve cellular removal while preserving matrix structure in rat sciatic nerve sections. Standard freeze drying and unidirectional freeze drying were employed to impart the necessary pore architecture, and our results suggest that unidirectional freezing is a pertinent modification to the freeze drying process to obtain axially aligned channels. These highly porous scaffolds obtained using unidirectional freeze-drying possessed similar tensile properties to native nerve tissue and exhibited enhanced cellular penetration after 14 days of culture when compared to non-freeze dried and standard freeze-dried scaffolds. The results of this study not only highlight the importance of aligned pores of diameters ~20-60μm on cellular infiltration, but also presents unidirectional freeze drying as a viable technique for producing this required architecture in decellularized nerves. To the best of our knowledge, this study represents the first attempt to manipulate the physical structure of decellularized nerves to enhance cell penetration which may serve as a basis for future peripheral nerve regenerative strategies using decellularized allografts.

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Section: Introductionmentioning

confidence: 99%

Decellularized grafts with axially aligned channels for peripheral nerve regeneration

Sridharan

Reilly

Buckley

2015

Journal of the Mechanical Behavior of Biomedical Materials

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“…This novel NC combined the biocompatibility of the collagen with the mechanical stability of the PCL, and was successfully used to repair 0.8 cm of nerve gap, achieving similar electrophysiological and muscle reinnervation results as the nerve autograft [113]. Motor recovery was also achieved with this biomaterial's combination in the reconstruction of 1 cm of defect in the tibial branch in rats [114]. Similar outcomes were achieved with the use of poly (L-lactide-co-caprolactone) nanofiber-based NC [115].…”

Section: Improvements Of the Ncs Compositionmentioning

confidence: 85%

Tissue engineering of the peripheral nervous system

Carriel¹,

Alaminos²,

Garzón³

et al. 2014

Expert Review of Neurotherapeutics

103

142

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The structure and function of peripheral nerves can be affected by a range of conditions with severe consequences in these patients. Currently, there are several surgical techniques available to treat peripheral nerve defects. Direct repair is the preferred treatment for short nerve gaps, and nerve autografting is the gold standard in critical nerve defects. The autografting is not always available, and the use of allograft, decellularized allograft and nerve conduits are often used with variable success. During the recent years, several outcomes were achieved in peripheral nerve tissue engineering. Promising experimental results have been demonstrated with this novel generation of nerve conduits, mainly composed by biodegradables materials in combination with intraluminal fillers, growth factors and different cell sources.

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“…Results revealed that axonal continuity was normally recovered 8 weeks after surgery, with the muscle function recovery occurring after 1-20 weeks, and recovery of donor nerve function occurring after 20 weeks. Therefore, the use of electrospun PCL/collagen nerve conduits appeared to have great potential as materials for complex peripheral motor nerve repair [73] (Figure 4).…”

Section: Lee Et Al Utilized Electrospinning Techniques To Prepare Pcmentioning

confidence: 99%

Electrospinning of Collagen and Its Derivatives for Biomedical Applications

Lü¹,

Guo²

2018

Novel Aspects of Nanofibers

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Collagen, gelatin and their derived polypeptides can act as multifunctional natural polymers with excellent physicochemical properties for biomedical applications. The use of electrospinning technology can convert collagen materials into nanofibrous materials that exhibit porous micro-nanostructures with good mechanical properties and excellent biocompatibility profiles. In this chapter, a systematic review of collagen electrospinning is presented and related applications are introduced including tissue engineering (e.g., artificial skin, artificial vasculature, cartilage repair, etc.), drug delivery, hemostatic dressings, periodontal restoration, biofilms, and wound dressings will now be discussed.

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End-to-side neurorrhaphy using an electrospun PCL/collagen nerve conduit for complex peripheral motor nerve regeneration

Cited by 89 publications

References 47 publications

Decellularized grafts with axially aligned channels for peripheral nerve regeneration

Decellularized grafts with axially aligned channels for peripheral nerve regeneration

Tissue engineering of the peripheral nervous system

Electrospinning of Collagen and Its Derivatives for Biomedical Applications

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