Endocannabinoids in aqueous humour of patients with or without diabetes

Richardson, Patrick; Ortori, Catharine A.; Barrett, D. A.; O’Sullivan, Saoirse E.; Idris, Iskandar

doi:10.1136/bmjophth-2019-000425

Cited by 6 publications

(1 citation statement)

References 38 publications

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“…Preclinical stages of DR have been investigated, in order to detect the very early drivers of retinopathy [85][86][87][88][89][90][91][92][93]. Chiang SY et al, for example, compared diabetic patients without and with clinical signs of DR and demonstrated increased levels of total protein in the AH but also a different AH protein profile in DR patients [85].…”

Section: Preclinical and Clinical Non-proliferative Diabetic Retinopathymentioning

confidence: 99%

Intraocular fluid biomarkers (liquid biopsy) in human diabetic retinopathy

Midena

Frizziero

Midena

et al. 2021

Graefes Arch Clin Exp Ophthalmol

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Purpose This article aims to review the impact of detecting and quantifying intraocular biomarkers (liquid biopsy) in both aqueous and vitreous humor in eyes of people affected by diabetes mellitus. Methods This is a detailed review about aqueous and/or vitreous humor sampling in human diabetic eyes for proteomic and/or metabolomic analysis contributing to the understanding of the pathophysiology and treatment effects of diabetic retinopathy. Results Aqueous and vitreous humor molecular biomarkers proved to be directly correlated to each other and valuable to study retinal conditions. Moreover, proteomic and metabolomic analysis showed that the biomarkers of neuroinflammation, neurodegeneration, and vasculopathy are detectable in intraocular fluids and that their concentration changes in different stages of disease, and in response to treatment of all diabetic retinopathy aspects, mainly diabetic macular edema and proliferative retinopathy. Conclusions Liquid biopsy offers the possibility to improve our knowledge of intraocular eye disease induced by diabetes mellitus. The exact quantification of intraocular biomarkers contributes to the precision medicine approach even in the diabetic retinopathy scenario. The diffusion of this approach should be encouraged to have quantifiable information directly from the human model, which may be coupled with imaging data.

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Section: Preclinical and Clinical Non-proliferative Diabetic Retinopathymentioning

confidence: 99%

Intraocular fluid biomarkers (liquid biopsy) in human diabetic retinopathy

Midena

Frizziero

Midena

et al. 2021

Graefes Arch Clin Exp Ophthalmol

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Advances in targeted liquid chromatography‐tandem mass spectrometry methods for endocannabinoid and N‐acylethanolamine quantification in biological matrices: A systematic review

Amir Hamzah,

Turner,

Nichols

et al. 2024

Mass Spectrometry Reviews

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Liquid chromatography paired with tandem mass spectrometry (LC‐MS/MS) is the gold standard in measurement of endocannabinoid concentrations in biomatrices. We conducted a systematic review of literature to identify advances in targeted LC‐MS/MS methods in the period 2017–2024. We found that LC‐MS/MS methods for endocannabinoid quantification are relatively consistent both across time and across biomatrices. Recent advances have primarily been in three areas: (1) sample preparation techniques, specific to the chosen biomatrix; (2) the range of biomatrices tested, recently favoring blood matrices; and (3) the breadth of endocannabinoid and endocannabinoid‐like analytes incorporated into assays. This review provides a summary of the recent literature and a guide for researchers looking to establish the best methods for quantifying endocannabinoids in a range of biomatrices.

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Minireview: Insights into the role of TRP channels in the retinal circulation and function

Thebault

2021

Neuroscience Letters

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Endocannabinoids in aqueous humour of patients with or without diabetes

Cited by 6 publications

References 38 publications

Intraocular fluid biomarkers (liquid biopsy) in human diabetic retinopathy

Intraocular fluid biomarkers (liquid biopsy) in human diabetic retinopathy

Advances in targeted liquid chromatography‐tandem mass spectrometry methods for endocannabinoid and N‐acylethanolamine quantification in biological matrices: A systematic review

Minireview: Insights into the role of TRP channels in the retinal circulation and function

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