Endochondral bone formation in gelatin methacrylamide hydrogel with embedded cartilage-derived matrix particles

Visser, Jetze; Gawlitta, Debby; Benders, K.E.M.; Toma, Selynda M.H.; Pouran, Behdad; Weeren, P. R. van; Dhert, Wouter J.A.; Malda, Jos

doi:10.1016/j.biomaterials.2014.10.020

Cited by 162 publications

(158 citation statements)

References 49 publications

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“…Cartilage canals within this cartilaginous precursor act as conduits for vascular invasion to enable its conversion into bone [9] . It has been demonstrated that cartilaginous templates generated in vitro using adult mesenchymal stem cells (MSCs) are vascularised and form bone following implantation [10][11][12][13] , suggesting that such engineered tissues could be used for the reconstruction of large bone defects. A central challenge with the translation of such developmentally inspired engineering strategies is ensuring that these immature soft tissues, which are designed to function in the relatively low load bearing environment of the developing limb, are provided with the necessary mechanical support to execute their function in the adult body.…”

Section: Introductionmentioning

confidence: 99%

3D Bioprinting of Developmentally Inspired Templates for Whole Bone Organ Engineering

Daly

Cunniffe

Sathy

et al. 2016

Adv Healthcare Materials

224

179

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The ability to print defined patterns of cells and extracellular-matrix components in three dimensions has enabled the engineering of simple biological tissues, however bioprinting functional solid organs is beyond the capabilities of current biofabrication technologies. An alternative approach would be to bioprint the developmental precursor to an adult organ, using this engineered rudiment as a template for subsequent organogenesis in vivo. Here we demonstrate that developmentally inspired hypertrophic cartilage templates can be engineered in vitro using stem cells within a supporting gamma-irradiated alginate bioink incorporating Arg-Gly-Asp (RGD) adhesion peptides.Furthermore, these soft tissue templates can be reinforced with a network of printed polycaprolactone fibres, resulting in a ~350 fold increase in construct compressive modulus providing the necessary stiffness to implant such immature cartilaginous rudiments into load bearing locations. As a proofof-principal, multiple-tool biofabrication was used to engineer a mechanically reinforced cartilaginous template mimicking the geometry of a vertebral body, which in vivo supported the development of a vascularized bone organ containing trabecular-like endochondral bone with a supporting marrow structure. Such developmental engineering approaches could be applied to the biofabrication of other solid organs by bioprinting pre-cursors that have the capacity to mature into their adult counterparts over time in vivo.3

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Section: Introductionmentioning

confidence: 99%

3D Bioprinting of Developmentally Inspired Templates for Whole Bone Organ Engineering

Daly

Cunniffe

Sathy

et al. 2016

Adv Healthcare Materials

224

179

View full text Add to dashboard Cite

show abstract

“…[35][36][37] Moreover, ECM particles have also been used to functionalize other biomaterials in order to enhance chondrogenesis. [38][39][40] …”

Section: Fibrinmentioning

confidence: 99%

Liu¹,

Yu²

2017

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Osteoarthritis has been a common disease among elderly people, but there is not a solution clinically efficient for osteoarthritis. Therefore, tissue engineering has been developed in the field of cartilage regeneration. Tissue engineering has three basic elements: cell, scaffold and growth factor. An injectable scaffold, which can form a gel with original liquid form at the site of defect by injection, is an emerging scaffold for cartilage engineering. A scaffold can be composed of many materials such as natural materials, synthetic materials, composite materials, as well as hybrid materials. This paper reviews natural materials for cartilage regeneration.

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“…However, adequate cues to control MSC fate have to be provided, as these cells have the tendency to progress into hypertrophic chondrogenesis and to give rise to bone formation via the endochondral pathway once implanted in vivo. 26 Combining MSCs with chondrocytes or chondrons has shown promising results both in vitro and in vivo, in which it seems that the MSCs stimulate and direct the chondrocytes/chondrons to synthesize new cartilage-like tissue. [27][28][29] Furthermore, alternative cell populations with regenerative potential are being investigated, including subpopulations of chondroprogenitor cells, which can be harvested from mature cartilage and can be expanded in mono-layer culture without losing their chondrogenic phenotype.…”

Section: Cell Laden and Bioactive Inksmentioning

confidence: 99%

“…Osteochondral constructs have, for example, been successfully generated with either osteoblasts in the bone part and chondrocytes in the cartilage part, 48 or stem cells in both layers with additional biological cues to induce bone differentiation in the one part and cartilage formation in the other. 26,49 However, in the latter study further evaluation of biological cues is necessary, as the MSCs generated bone via the endochondral pathway in the cartilage layer after in vivo implantation. 26 Furthermore, to mimic the bone compartment, bio-inks that can both carry cells and harden over time have been developed, to achieve a stiffness within the same range of those of cancellous bone.…”

Section: Shape Architecture and Multiphasic Organizationmentioning

confidence: 99%

“…26,49 However, in the latter study further evaluation of biological cues is necessary, as the MSCs generated bone via the endochondral pathway in the cartilage layer after in vivo implantation. 26 Furthermore, to mimic the bone compartment, bio-inks that can both carry cells and harden over time have been developed, to achieve a stiffness within the same range of those of cancellous bone. 38 Such bio-inks could be combined with hydrogels described above to obtain fully printed, cellular composites with mechanical properties that appropriately match bone and cartilage regions.…”

Section: Shape Architecture and Multiphasic Organizationmentioning

confidence: 99%

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Three-Dimensional Bioprinting and Its Potential in the Field of Articular Cartilage Regeneration

et al. 2016

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Three-dimensional (3D) bioprinting techniques can be used for the fabrication of personalized, regenerative constructs for tissue repair. The current article provides insight into the potential and opportunities of 3D bioprinting for the fabrication of cartilage regenerative constructs. Although 3D printing is already used in the orthopedic clinic, the shift toward 3D bioprinting has not yet occurred. We believe that this shift will provide an important step forward in the field of cartilage regeneration. Three-dimensional bioprinting techniques allow incorporation of cells and biological cues during the manufacturing process, to generate biologically active implants. The outer shape of the construct can be personalized based on clinical images of the patient’s defect. Additionally, by printing with multiple bio-inks, osteochondral or zonally organized constructs can be generated. Relevant mechanical properties can be obtained by hybrid printing with thermoplastic polymers and hydrogels, as well as by the incorporation of electrospun meshes in hydrogels. Finally, bioprinting techniques contribute to the automation of the implant production process, reducing the infection risk. To prompt the shift from nonliving implants toward living 3D bioprinted cartilage constructs in the clinic, some challenges need to be addressed. The bio-inks and required cartilage construct architecture need to be further optimized. The bio-ink and printing process need to meet the sterility requirements for implantation. Finally, standards are essential to ensure a reproducible quality of the 3D printed constructs. Once these challenges are addressed, 3D bioprinted living articular cartilage implants may find their way into daily clinical practice.

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Endochondral bone formation in gelatin methacrylamide hydrogel with embedded cartilage-derived matrix particles

Cited by 162 publications

References 49 publications

3D Bioprinting of Developmentally Inspired Templates for Whole Bone Organ Engineering

3D Bioprinting of Developmentally Inspired Templates for Whole Bone Organ Engineering

Three-Dimensional Bioprinting and Its Potential in the Field of Articular Cartilage Regeneration

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Endochondral bone formation in gelatin methacrylamide hydrogel with embedded cartilage-derived matrix particles

Cited by 162 publications

References 49 publications

3D Bioprinting of Developmentally Inspired Templates for Whole Bone Organ Engineering

3D Bioprinting of Developmentally Inspired Templates for Whole Bone Organ Engineering

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Three-Dimensional Bioprinting and Its Potential in the Field of Articular Cartilage Regeneration

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