Endocrine and genetic control of sex differentiation in the Malacostracan Crustacea

Katakura, Yasutoshi

doi:10.1080/07924259.1989.9672075

Cited by 43 publications

(18 citation statements)

References 8 publications

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“…(Endocrinology 150: 1278 -1286, 2009) E ver since it was first proposed as the source of a hypothetical masculinizing hormone in crustaceans, the androgenic gland (AG) has been studied thoroughly in many crustacean species. The consensus emerging from these studies is that the AG plays a unifying role in the bewilderingly varied sex differentiation mechanisms in crustaceans (1)(2)(3)(4)(5).…”

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confidence: 99%

Temporal Silencing of an Androgenic Gland-Specific Insulin-Like Gene Affecting Phenotypical Gender Differences and Spermatogenesis

et al. 2008

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Androgenic glands (AGs) of the freshwater prawn Macrobrachium rosenbergii were subjected to endocrine manipulation, causing them to hypertrophy. Transcripts from these glands were used in the construction of an AG cDNA subtractive library. Screening of the library revealed an AG-specific gene, termed the M. rosenbergii insulin-like AG (Mr-IAG) gene. The cDNA of this gene was then cloned and fully sequenced. The cysteine backbone of the predicted mature Mr-IAG peptide (B and A chains) showed high similarity to that of other crustacean AG-specific insulin-like peptides. In vivo silencing of the gene, by injecting the prawns with Mr-IAG double-stranded RNA, temporarily prevented the regeneration of male secondary sexual characteristics, accompanied by a lag in molt and a reduction in growth parameters, which are typically higher in males of the species. In terms of reproductive parameters, silencing of Mr-IAG led to the arrest of testicular spermatogenesis and of spermatophore development in the terminal ampullae of the sperm duct, accompanied by hypertrophy and hyperplasia of the AGs. This study constitutes the first report of the silencing of a gene expressed specifically in the AG, which caused a transient adverse effect on male phenotypical gender differences and spermatogenesis.

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confidence: 99%

Temporal Silencing of an Androgenic Gland-Specific Insulin-Like Gene Affecting Phenotypical Gender Differences and Spermatogenesis

et al. 2008

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“…The nature of the genomic region revealed in this study (a unique sequence, dimorphic in female prawns and monomorphic in male prawns) has led us to hypothesize that this region resides in the sex chromosome in a locus common to the W and Z chromosomes. This idea is in keeping with the heterogametic female theory of Katakura (1989). However, as the sex chromosome of M. rosenbergii is not distinguishable morphologically (Justo et al, 1991), further research is needed to verify this notion.…”

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confidence: 78%

Isolation and characterization of a female-specific DNA marker in the giant freshwater prawn Macrobrachium rosenbergii

et al. 2011

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In this study, a female-specific DNA marker in the freshwater prawn Macrobrachium rosenbergii was identified through amplified fragment length polymorphism (AFLP). The AFLPderived sequence-characterized amplified region (SCAR) marker was tested in over 200 individuals, giving reproducible sex identification. Further molecular characterization of the sex-marker's genomic region (B3 kb long) revealed the presence of tandem and inverted repeats. The B3-kb sequence was identified both in male and female prawns, but with subtle differences: a deletion of 3 bp (present in female prawn but absent in male prawn) identified upstream of the SCAR marker sequence and two female-specific single-nucleotide polymorphisms, both indicating that male prawns are homozygous, whereas female prawns are heterozygous in this locus. Fluorescent in situ hybridization showed the B3-kb sequence to be unique: to the best of our knowledge, this is the first report of a unique sex-specific sequence observed in situ in crustaceans. The sex-specific marker identified in M. rosenbergii may have considerable applied merit for crustacean culture in that it will enable the determination of genetic sex at early developmental stages when phenotypic differences are not identifiable.

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“…It has been shown that AGH is essential for proper development of gonoducts in malacostracan crustaceans (Katakura 1989), and it is also responsible for male-type morphotype development in M. rosenbergii (Okumura & Hara 2004). Androgenic gland hormone also controls synthesis of vitellogenin (precursor of a major egg protein) as documented in C. quadricarinatus .…”

Section: Gonadsmentioning

confidence: 98%

Endocrine regulation of the reproduction in crustaceans: Identification of potential targets for toxicants and environmental contaminants

et al. 2008

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Progress in ecotoxicological research documents that crustaceans are highly vulnerable to diverse chemicals and toxicants in the environment. In particular, pollutants affecting endocrine homeostasis in crustaceans (i.e., endocrine disruptors) are intensively studied, and serious reproductive disorders have been documented. In this review, current knowledge about the endocrine regulation of the crustacean reproduction is put together with the published ecotoxicological data with an attempt to summarize the potential of xenobiotics to affect crustacean reproduction. Following gaps and trends were identified: (1) Studies are required in the field of neurohormone (serotonin and dopamine) regulation of the reproduction and possible modulations by environmental toxicants such as antidepressant drugs. (2) Molting-related parameters (regulated by ecdysteroid hormones) are closely coordinated with the development and reproduction cycles in crustaceans (cross-links with methyl farnesoate signalling), and their susceptibility to toxicants should be studied. (3) Other biochemical targets for xenobiotics were recently discovered in crustaceans and these should be explored by further ecotoxicological studies (e.g., new information about ecdysteroid receptor molecular biology). (4) Some sex steroid hormones known from vertebrates (testosterone, progesterone) have been reported in crustaceans but knowledge about their targets (crustacean steroid receptors) and signalling is still limited. (5) Determination of the sex in developing juveniles (affecting the sex ratio in population) is a sensitive parameter to various xenobiotics (including endocrine disruptors) but its modulation by general environmental stress and non-specific toxicity should be further studied.

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Endocrine and genetic control of sex differentiation in the Malacostracan Crustacea

Cited by 43 publications

References 8 publications

Temporal Silencing of an Androgenic Gland-Specific Insulin-Like Gene Affecting Phenotypical Gender Differences and Spermatogenesis

Temporal Silencing of an Androgenic Gland-Specific Insulin-Like Gene Affecting Phenotypical Gender Differences and Spermatogenesis

Isolation and characterization of a female-specific DNA marker in the giant freshwater prawn Macrobrachium rosenbergii

Endocrine regulation of the reproduction in crustaceans: Identification of potential targets for toxicants and environmental contaminants

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