Endocrine Cells in Human Intestine: An Immunocytochemical Study

Sjölund, K.; Sanden, G. van der; Håkanson, R.; Sundler, F.

doi:10.1016/s0016-5085(83)80080-8

Cited by 457 publications

(184 citation statements)

References 35 publications

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“…a 0 values do not mean the absence of this cell type, but imply that in the randomly chosen fields, such cells did not occur. colon of controls was not statistically different is in agreement with previous results obtained with both morphometry [26] and radioimmunoassay [17]. It should be remembered, however, that the tissue specimens used as controls were obtained from patients with malignancy and who were older than UC and CD patients.…”

Section: Discussionsupporting

confidence: 90%

Colonic endocrine cells in inflammatory bowel disease

Danielsson¹,

Stenling

Grimelius

1997

Journal of Internal Medicine

210

137

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Section: Discussionsupporting

confidence: 90%

Colonic endocrine cells in inflammatory bowel disease

Danielsson¹,

Stenling

Grimelius

1997

Journal of Internal Medicine

210

137

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“…Enteroendocrine cells make up less than 1 percent of intestinal epithelial cells, but comprise the largest and most complex endocrine organ of the mammalian body (Dayal, 1991). Immunohistochemical methods have demonstrated at least 16 different subpopulations of enteroendocrine cells, based on major peptide andor amine products (Cheng and Leblond, 1974b;Lewin, 1986;Sjolund et al, 1983). The products of the enteroendocrine cells have multiple roles in the normal physiological functioning of the gastrointestinal tract, including effects on motility and pancreatic and biliary secretions (Dayal, 1991).…”

Section: Introductionmentioning

confidence: 99%

“…The products of the enteroendocrine cells have multiple roles in the normal physiological functioning of the gastrointestinal tract, including effects on motility and pancreatic and biliary secretions (Dayal, 1991). Each subpopulation has a distinct distribution along both the cephalo-caudal and crypt-villus axes (Sjolund et al, 1983;. The distribution of enteroendocrine cell subpopulations is constantly reestablished as enteroendocrine cells differentiate from pluripotent stem cells, which reside in the crypts of Lieberkiihn.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical studies indicate multiple enteroendocrine cell differentiation pathways in the mouse proximal small intestine

Aiken

Kisslinger

Roth

1994

Developmental Dynamics

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The enteroendocrine cell system of the mammalian gastrointestinal tract is comprised of at least 16 different subpopulations. Each subpopulation shows a characteristic distribution along both the crypt-villus and cephalocaudal axes. In both the small intestine and colon of adult mice, multilabel immunohistochemistry has demonstrated that two or more neuroendocrine products can be coexpressed in various combinations in single cells along the crypt-villus axis, suggesting that enteroendocrine phenotypes may be actively regulated. Using bromodeoxyuridine (BrdU) incorporation and multilabel immunohistochcmistry, we have previously demonstrated an enteroendocrine cell differentiation pathway consisting of two subpopulations of cells in the mouse proximal small intestinmne involving the sequential expression of substance P, serotonin, and secretin in cells migrating out of the crypts into the villi, and a second involving the expression of substance P and serotonin in cells which remain in the crypts. In this report, we use double label immunohistochemistry and BrdU incorporation to define the temporal and spatial interrelationships between gastrin, cholecystokinin (CCK), glucagon-like peptide-1 (GLP-I), and gastric inhibitory peptide (GIP) immunoreactive cells in the mouse proximal small intestine. The expression of these products was compared with that of substance P, serotonin, and secretin. Minimal overlap of expression was found in cells immunoreactive for substance P or serotonin with gastrin, CCK, GLP-1, or GIP; however, secretin was found colocalized in villus-associated gastrin, CCK, and GLP-1 containing cells. We demonstrate that, similar to the bidirectionally migrating substance P and serotonin expressing cells, gastrin, CCK, GLP-1, and secretin are expressed in upwardly migrating cells, and gastrin, CCK, and GLP-1 are expressed in downwardly migrating cells that fail to express secretin. GIP containing cells only rarely coexpressed any of the products examined, but were found both in the villi and the crypts, suggesting both upwardly and downwardly migrating populations. These findings demonstrate several novel enteroendocrine cell differentiation pathways. In addition, the expression of secretin 0 1994 WILEY-LISS, INC.in the villi, but not in the crypts, by two otherwise distinct differentiation pathways, and the lack of secretin expression by villus-associated GIP expressing cells, suggests that local factors present in the crypts and/or on the villi are necessary, but not sufficient, for secretin expression.

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“…The enteroendocrine system of the mammalian gut has been called the largest and most complex endocrine organ of the body (Dayal, 1991). Although enteroendocrine cells make up less than 1% of intestinal epithelial cells, immunohistochemical methods to detect peptide and amine products have demonstrated a t least 16 different subpopulations (Cheng and Leblond, 197413;Lewin, 1986;Sjolund et al, 1983). The polypeptide hormones and biogenic amines secreted by the endocrine cells act as chemical messengers that participate in the control of the normal secretory, absorptive, and motor functions of the gut (Dayal, 1991).…”

Section: Introductionmentioning

confidence: 99%

“…The polypeptide hormones and biogenic amines secreted by the endocrine cells act as chemical messengers that participate in the control of the normal secretory, absorptive, and motor functions of the gut (Dayal, 1991). In order to produce an integrated response to various discontinuous stimuli, each subpopulation has a distinct distribution along both the cephalo-caudal and crypt to villus axes (Sjolund et al, 1983;. For example, the duodenum and proximal jejunum, which are sites of complex secretory and absorptive processes, contain large numbers of many different types of endocrine cells; in comparison, the colon, which is much less active in these functions, has fewer, less diverse endocrine cell types (Dayal, 1991).…”

Section: Introductionmentioning

confidence: 99%

Temporal differentiation and migration of substance P, serotonin, and secretin immunoreactive enteroendocrine cells in the mouse proximal small intestine

Aiken

Roth

1992

Developmental Dynamics

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Precise spatial interrelationships exist between substance P, serotonin, and secretin containing enteroendocrine cells in the gastrointestinal tract of mice. In the proximal small intestine these products are coexpressed in various combinations in single enteroendocrine cells along the crypt to villus axis in a pattern that suggests the sequential expression of substance P, serotonin, and secretin. In this report we use bromodeoxyuridine (BrdU) and multilabeling immunohistochemistry to define the temporal and spatial interrelationships between substance P, serotonin, and secretin immunoreactive cells in the mouse proximal small intestine. Our findings demonstrate the sequential expression of substance P, serotonin, and secretin in a population of upwardly migrating enteroendocrine cells and, furthermore, identify a population of crypt associated cells coexpressing substance P and serotonin that fails to traverse this pathway. The lack of secretin immunoreactive cells in the crypts suggests that local factors present in the crypts and/or on villi regulate secretin expression. The combined use of BrdU and multilabeling immunohistochemistry provides a method for defining enteroendocrine cell differentiation pathways throughout the gastrointestinal tract. 0 1992 Wiley-Liss, Inc.

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Endocrine Cells in Human Intestine: An Immunocytochemical Study

Cited by 457 publications

References 35 publications

Colonic endocrine cells in inflammatory bowel disease

Colonic endocrine cells in inflammatory bowel disease

Immunohistochemical studies indicate multiple enteroendocrine cell differentiation pathways in the mouse proximal small intestine

Temporal differentiation and migration of substance P, serotonin, and secretin immunoreactive enteroendocrine cells in the mouse proximal small intestine

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