Endocrine Disruption, Cytotoxicity and Genotoxicity of an Organophosphorus Insecticide

Belaid, Afifa; Methneni, Nosra; Nasri, Emna; Bchir, Sarra; Anthonissen, Roel; Verschaeve, Luc; Tilly, Véronique Le; Turco, Vincenzo Lo; Bella, Giuseppa Di; Mansour, Hédi Ben; Khdary, Nezar H.

doi:10.3390/su132011512

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…In addition to the use of ecotoxicity, cytotoxicity and genotoxicity tests with the aim of assessing the potential risks from contact with pesticides at different levels of biological organization [ 52 ], the application of new molecular approaches to contribute to the elucidation of possible mechanisms through which pesticides induce different diseases, including cancer, is recommended. Deregulation of miRNAs expression (let-7, miR-9, miR-21, miR-30, miR-126 miR-155, miR-181, miR-223, miR-363 and miR-320), has been evaluated to be a leading biomarker in evaluating the response to pesticide exposure [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Pesticide Exposure over DNA Damage in Farmers from Los Reyes, Michoacan in Mexico

Valencia-Quintana

Milić

Bonassi

et al. 2023

Toxics

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In the municipality of Los Reyes, Michoacán, in Mexico, several economic activities coexist; however, the most relevant is agriculture. It stands out as an agro-industrial center and commercial enclave in the region, suitable for the cultivation of sugar cane; however, currently fruit growing takes first place with blackberry, raspberry and blueberry, followed by avocado, peach, strawberry and other crops. A large quantity and variety of pesticides are applied to crops, consequently the population is at constant risk. This study aimed to evaluate whether pesticides are a factor in genetic damage to agricultural workers from Los Reyes, Michoacán, using alkaline comet assay. Fifty-nine residents participated (41 workers and 18 controls). Results included confounding factors (alcohol consumption, smoking habit, gender, age, BMI, etc.) indicated a non-significant statistical difference between two groups, with higher DNA damage values in workers that was higher than the values expected in a normal healthy unexposed population. It seems that the control measures, safe handling of pesticides and quality standards, required by the producers so that their products can be exported, have resulted in less damage, despite workers’ activity, but higher damage than the reference values still requires regular surveillance of those exposed. The use of protective equipment or measures can reduce the risk of damage, so it is also necessary to promote their service and comply with labor regulations for agricultural workers.

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Section: Discussionmentioning

confidence: 99%

Effect of Pesticide Exposure over DNA Damage in Farmers from Los Reyes, Michoacan in Mexico

Valencia-Quintana

Milić

Bonassi

et al. 2023

Toxics

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“…The cytotoxicity of the extract followed a similar trend to the results of other assays in this study. Cytotoxicity is a non-specific endpoint of toxicity and can be applied as an early warning sign since it is a reflection of the toxic response elicited by chemicals present in mixtures as in the case of plant extracts [72] Cell death is reflected by a slowdown in metabolic activity [34] and can be measured using various viability assays. In this study, the cytotoxic potential of B. pilosa water and ethanol extracts were assessed using different cell lines: HepG2 and Vero.…”

Section: Discussionmentioning

confidence: 99%

“…Cell lines from the human liver, HepG2 (cancerous), and African monkey kidney, Vero (non-cancerous), were used to measure the cytotoxic potential of B. pilosa extracts (water and 70% ethanol). Since different cell lines originate from different organ tissues and these organs have different sensitives towards compounds, the measured cytotoxic responses may be dependent on the cell type used [34]. The HepG2 liver cell line was chosen because one of the main functions of the liver is detoxification.…”

Section: Maintenance Of Cellsmentioning

confidence: 99%

Phytochemical-Based Evidence of the Health Benefits of Bidens Pilosa Extracts and Cytotoxicity

et al. 2023

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Purpose Bidens pilosa L. is traditionally used as a flavouring agent in foods, in the treatment of diseases, in agriculture as a biopesticide and herbicide, and in the phytoremediation of soils contaminated with heavy metals. The vast range of uses of B. pilosa for a variety of purposes is questionable, hence motivating the objectives of this study, which are to assess the cytotoxicity, health benefits, and/or risks of B. pilosa using chemical-based evidence. Methods A real-time cell analysis (xCELLigence system), ultra-high-performance liquid chromatography coupled to a quadrupole-time-of-flight mass spectrometer, high-performance liquid chromatography with a diode array detector, and chemical-based spectrophotometric methods were adopted in the study. Results High concentrations of the ethanol extracts exhibited cytotoxic activity on HepG2 (cancerous), and Vero (non-cancerous) cell lines, whereas the water extracts promoted cell proliferation at selected concentrations. The chemical profiling enabled the separation as well as characterisation of 137 phytochemicals. These were mainly phenolic acids, flavonoids, fatty acids, coumarins, and furanocoumarins. There was no toxic compound identified. Conclusion The ethanol extracts are generally more potent and exhibit stronger antioxidant activity and cytotoxicity, probably due to the presence of more flavonoids and phenolic acids, validating the uses of B. pilosa and its relevance as a source of functional phytochemicals.

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“…The cytotoxicity of OPs is mainly characterized by the inhibition of cell proliferation and the induction of apoptosis [21]. Long non-coding RNAs are widely involved in physiological processes such as cellular stress response, proliferation, and apoptosis [22,23].…”

Section: Discussionmentioning

confidence: 99%

Tris (2-chloroisopropyl) phosphate and Tris (nonylphenyl) phosphite Promote Human Renal Cell Apoptosis through the ERK/CEPBA/Long Non-Coding RNA Cytoskeleton Regulator Axis

Zhao,

Yan,

Gao

et al. 2024

Toxics

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Organophosphorus compounds (OPs) are widely used and have the potential to be harmful environmental toxicants to humans. Long non-coding RNA (lncRNA) plays a crucial regulatory role in cytotoxicity. This study aimed to investigate the effects of OPs on the expression of lncRNAs in cells. The effects of the industrial OPs TNPP and TCPP on both CYTOR and cellular viability were examined in the following human renal cell lines: HEK293T and HK-2. Both TCPP and TNPP downregulated CYTOR expression, increased reactive oxygen species levels, and induced apoptosis; the upregulated expression of CYTOR resulted in a reduction in apoptosis. The results of the luciferase reporter assay and the knock-down assay indicate that CEBPA binds to the upstream promoter region of CYTOR and regulates its transcription. Furthermore, TCPP and TNPP were found to downregulate the phosphorylation of ERK in the signaling pathway that is upstream of CEBPA. These results indicate that TCPP and TNPP can decrease the level of CEBPA by reducing ERK phosphorylation; this leads to a decrease in CYTOR expression, which further promotes cellular reactive oxygen species and apoptosis. Therefore, the ERK/CEBPA/CYTOR axis is one of the pathways by which organophosphates produce cytotoxicity, leading to renal cell injury. This study presents evidence for both the abnormal expression of lncRNA that is caused by organophosphates and the regulatory function of lncRNA regarding downstream cellular viability.

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Endocrine Disruption, Cytotoxicity and Genotoxicity of an Organophosphorus Insecticide

Cited by 5 publications

References 38 publications

Effect of Pesticide Exposure over DNA Damage in Farmers from Los Reyes, Michoacan in Mexico

Effect of Pesticide Exposure over DNA Damage in Farmers from Los Reyes, Michoacan in Mexico

Phytochemical-Based Evidence of the Health Benefits of Bidens Pilosa Extracts and Cytotoxicity

Tris (2-chloroisopropyl) phosphate and Tris (nonylphenyl) phosphite Promote Human Renal Cell Apoptosis through the ERK/CEPBA/Long Non-Coding RNA Cytoskeleton Regulator Axis

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