Endocrine pancreas development in zebrafish

Tehrani, Zahra; Lin, Shuo

doi:10.4161/cc.10.20.17764

Cited by 39 publications

(36 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Larvae are transparent, allowing easy visualization of genetic markers or fluorescent dyes [14,45]. Zebrafish develop metabolic abnormalities when fed high-fat diets [46,47], show similar complications of persistently high glucose and have many of the same transcription factor pathways involved in development of endocrine, liver and other organs important in controlling metabolism [44,48]. While zebrafish may thus offer major advantages for screening diabetes modifiers, details of comparative organ biology must first be evaluated and further studies like those we describe here are required.…”

Section: Discussionmentioning

confidence: 99%

Expression and function of ATP-dependent potassium channels in zebrafish islet β-cells

et al. 2017

View full text Add to dashboard Cite

ATP-sensitive potassium channels (KATP channels) are critical nutrient sensors in many mammalian tissues. In the pancreas, KATP channels are essential for coupling glucose metabolism to insulin secretion. While orthologous genes for many components of metabolism–secretion coupling in mammals are present in lower vertebrates, their expression, functionality and ultimate impact on body glucose homeostasis are unclear. In this paper, we demonstrate that zebrafish islet β-cells express functional KATP channels of similar subunit composition, structure and metabolic sensitivity to their mammalian counterparts. We further show that pharmacological activation of native zebrafish KATP using diazoxide, a specific KATP channel opener, is sufficient to disturb glucose tolerance in adult zebrafish. That β-cell KATP channel expression and function are conserved between zebrafish and mammals illustrates the evolutionary conservation of islet metabolic sensing from fish to humans, and lends relevance to the use of zebrafish to model islet glucose sensing and diseases of membrane excitability such as neonatal diabetes.

show abstract

Section: Discussionmentioning

confidence: 99%

Expression and function of ATP-dependent potassium channels in zebrafish islet β-cells

et al. 2017

View full text Add to dashboard Cite

show abstract

“…We therefore injected the yolk of embryos with very high levels of glucose, in order to determine if this early glucose stimulus could durably modify the genomic expression at later larval stages. Organogenesis begins early in zebrafish, and before the onset of exogenous feeding (5-6 dpf) all major organ systems are formed and partially functional: eyes, gut, liver, pancreas among others (Holmberg et al, 2004;Tao and Peng, 2009;Tehrani and Lin, 2011;Wallace and Pack, 2003). However, attention should be given to determining the delicate balance between the establishment of complex metabolic…”

Section: Expression Of Metabolic Genes In Embryos and Larvaementioning

confidence: 99%

Glucose overload in yolk has little effects on the long term modulation of carbohydrate metabolic genes in zebrafish (Danio rerio)

Rocha

Dias

Engrola

et al. 2013

Journal of Experimental Biology

View full text Add to dashboard Cite

Some fish show a low metabolic ability to use dietary carbohydrates. The use of early nutritional stimuli to program metabolic pathways in fish is ill defined. Therefore, studies were undertaken with zebrafish to assess the effect of high glucose levels during the embryonic stage as a lifelong modulator of genes involved in carbohydrate metabolism. Genes related to carbohydrate metabolism were expressed at low levels at 0.2 and 1 day post-fertilization (dpf). However, from 4 dpf onwards there was a significant increase on expression of all genes, suggesting that all analysed pathways were active. By microinjection, we successfully enriched zebrafish egg yolk with glucose (a 43-fold increase of basal levels). Acute effects of glucose injection on gene expression were assessed in larvae up to 10 dpf, and the programming concept was evaluated in juveniles (41 dpf) challenged with a hyperglucidic diet. At 4 dpf, larvae from glucose-enriched eggs showed a downregulation of several genes related to glycolysis, glycogenolysis, lipogenesis and carbohydrate digestion in comparison with control (saline-injected) embryos. This inhibitory regulation was suppressed after 10 dpf. At the juvenile stage, and upon switching from a low to a high digestible carbohydrate diet, early glucose enrichment had no significant effect on most analysed genes. However, these same fish showed altered expression of the genes for cytosolic phosphoenolpyruvate carboxykinase, sodium-dependent glucose cotransporter 1 and glycogen synthase, suggesting changes to the glucose storage capacity in muscle and glucose production and transport in viscera. Overall, supplementation of egg yolk with high glucose levels had little effect on the long-term modulation of carbohydrate metabolic genes in zebrafish.

show abstract

“…24 Many of the signaling pathways and transcription factors regulating b-cell development in zebrafish are homologous to mammals. [24][25][26] However, unlike rodents, b-cell replication is rare in newly hatched embryos and free feeding larvae. Several groups reported that replicating b-cells are very rare in larvae at 3 dpf, 27 4-6 dpf, 22 and 6-8 dpf 18 using BrdU incorporation as a measure of proliferation.…”

Section: Introductionmentioning

confidence: 99%

Targeted Overexpression of CKI-Insensitive Cyclin-Dependent Kinase 4 Increases Functional β-Cell Number Through Enhanced Self-Replication in Zebrafish

Maddison²,

Crees³

et al. 2013

Zebrafish

View full text Add to dashboard Cite

In this study, we tested the hypothesis that insufficient activity of cyclin-dependent kinase 4 may be responsible for the low replication rate by ectopically expressing in b-cells a mutant CDK4 (CDK4 R24C ) that is insensitive to inhibition by cyclin-dependent kinase inhibitors. Our data show that expression of CDK4 R24C in b-cells enhanced b-cell replication. CDK4R24C also dampened compensatory b-cell neogenesis in larvae and improved glucose tolerance in adult zebrafish. Our data indicate that CDK4 inhibition contributes to the limited b-cell replication in larval zebrafish. To our knowledge, this is the first example of genetically induced b-cell replication in zebrafish.

show abstract

Endocrine pancreas development in zebrafish

Cited by 39 publications

References 71 publications

Expression and function of ATP-dependent potassium channels in zebrafish islet β-cells

Expression and function of ATP-dependent potassium channels in zebrafish islet β-cells

Glucose overload in yolk has little effects on the long term modulation of carbohydrate metabolic genes in zebrafish (Danio rerio)

Targeted Overexpression of CKI-Insensitive Cyclin-Dependent Kinase 4 Increases Functional β-Cell Number Through Enhanced Self-Replication in Zebrafish

Contact Info

Product

Resources

About