Endocrine profile and testicular histomorphometry in adult rat offspring of diabetic mothers

Jelodar, Gholamali; Khaksar, Zabihollah; Pourahmadi, Mohammad

doi:10.1007/s12576-009-0045-7

Cited by 45 publications

(49 citation statements)

References 22 publications

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“…It was observed that the therapies studied in this work succeeded in controlling the STZ-induced diabetic [24], pioglitazone [23] and sitagliptin [36,37]. Although the weights of testis, epididymis and seminal vesicle as well as testosterone hormone level was significantly reduced in the STZ-induced diabetic rats and these findings were consistent with those of previous studies [38][39][40][41], metformin succeeded to restore it back to values with insignificant difference compared to the NC group while the pioglitazone and sitagliptin failed to do that. Reduced serum testosterone in diabetic rats was attributed in previous studies to neuroendocrine dysfunction and the absence of the stimulatory effect of insulin on the LC [3,42].…”

Section: Discussionsupporting

confidence: 91%

“…The disorganized germinal epithelium and edematous thickened interstitial connective tissue in the testis of diabetic rats have been described in the previous studies [33,40,[42][43][44] and have been also observed in this study. The crowded nuclei and cytoplasmic vacuoles of the epithelial lining the epididymis of the DC observed in this study was in agreement with those of Soudamani et al [45] who attributed them to shrinkage of epididymal tubules.…”

Section: Discussionsupporting

confidence: 86%

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Impaired expression of sex hormone receptors in male reproductive organs of diabetic rat in response to oral antidiabetic drugs

Ayuob

Murad

Ali

2015

Folia Histochem Cytobiol.

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Introduction. Few oral antidiabetic drugs have been evaluated for their reproductive complication. This study aimed to evaluate the effect of metformin, pioglitazone and sitagliptin on the structure of male reproductive system through an immunohistopathological study. Material and methods. Sprague-Dawley male rats were injected with streptozotocin. The diabetic rats were divided into four groups (n = 8/each group); diabetic control, metformin-, pioglitazone-and sitagliptin-treated groups in addition to a normal control group (n = 8). At the end of the experiment, blood samples were collected for biochemical assessment. Testis, epididymis and seminal vesicle were dissected and processed for histopathological examination using routine and immune-staining. Results. All drugs significantly (p < 0.05) decreased fasting blood glucose, glycated hemoglobin, total cholesterol, triglycerides and malondialdehyde compared to the diabetic control group. Metformin has induced the least pathologic changes on the structure of the testis, epididymis and seminal vesicle among the studied drugs. Metformin succeeded to restore weights of testis, epididymis and seminal vesicle as well as testosterone hormone level back to values of the NC group while the pioglitazone and sitagliptin failed to do that. A significant reduction (p < 0.05) in testicular ERa and ERb immunoexpression of pioglitazone-treated group as well as suppression of ERb and AR immunoreactivity in in epididymus and seminal vesicles of pioglitazone-and sitagliptin-treated rats were observed compared to the control animals. Conclusions. Histological structure as well ER and AR expression in the system organs were negatively and significantly affected with all studied drugs. Metformin has the least effect on the structure of the studied male reproductive organs. Thus, pioglitazone and sitagliptin treatment should be avoided in young male diabetic patients.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 86%

Impaired expression of sex hormone receptors in male reproductive organs of diabetic rat in response to oral antidiabetic drugs

Ayuob

Murad

Ali

2015

Folia Histochem Cytobiol.

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“…Sperm concentration and motility were both significantly lower in diabetic compared with control rats (Kim and Moley, 2008). Finally, a recent study on the offspring of diabetic mothers showed that the number of spermatogonia (226.3%) and the diameters of the seminiferous tubules (211%) were significantly lower compared with controls (Jelodar et al, 2009).…”

Section: Animal Studiesmentioning

confidence: 94%

Diabetes Mellitus and Sperm Parameters

Vignera

Condorelli

Vicari

et al. 2012

Journal of Andrology

261

201

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Because of the paucity of studies and inconsistencies regarding the impact of diabetes mellitus (DM) on semen quality, this disease is seldom looked for in the infertile patient. Recently, this view has been challenged by findings showing that DM induces subtle molecular changes that are important for sperm quality and function. This brief review shows the main sperm parameters in patients with DM and presents the mechanisms hypothesized to explain the changes observed in these patients. The data available suggest that DM alters conventional sperm parameters. In addition, DM causes histologic damage of the epididymis, with a negative impact on sperm transit. Various mechanisms may explain the sperm damage observed in patients with DM. These include endocrine disorders, neuropathy, and increased oxidative stress. Many authors suggest that DM decreases serum testosterone levels. This is associated with a steroidogenetic defect in Leydig cells. In addition, diabetic neuropathy seems to cause atonia of seminal vesicles, bladder, and urethra. Furthermore, DM is associated with an increased oxidative stress, which damages sperm nuclear and mitochondrial DNA. Finally, spermatogenesis derangement and germ cell apoptosis in type 1 DM may relate to a local autoimmune damage, whereas insulin resistance, obesity, and other related comorbidities may impair sperm parameters and decrease testosterone serum levels in patients with type 2 DM.Key words: Fertility, hormone, infertility, semen analysis. J Androl 2012;33:145-153A lthough diabetes mellitus (DM) is known to cause many systemic complications, male infertility, based on impotence, retrograde ejaculation, and hypogonadism, is not widely recognized to be one of them. Because of the paucity of studies and inconsistencies regarding the impact of DM on semen quality, this disease is seldom looked for in the infertile patient. Recently, this view has been challenged by findings showing that DM induces subtle molecular changes that are important for sperm quality and function . In a retrospective analysis, we found a very high prevalence of subfertility (51%) among patients with diabetes (La Vignera et al, 2009a). Another study carried out in more than 500 male partners of infertile couples showed a prevalence of DM of <1.2% (Delfino et al, 2007). In a recent study, the prevalence of infertility in type 2 DM men was 35.1%. The prevalence of primary (16%) and secondary (19.1%) infertility was significantly higher in patients with diabetes compared with patients without diabetes. In addition, secondary infertility was higher than primary infertility. About half of the infertile men with diabetes were overweight, and 29.1% of them were obese. The smoking habit was more common in infertile men with diabetes (45.6%) than in fertile men with diabetes (33.6%). Statistical analysis confirmed that age, smoking habits, and obesity were the significant major contributors for infertility in men with diabetes. Obesity was the leading contributor for infertility. Other comorbid fa...

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“…В эксперименте на лабораторных животных показано, что ухудшение показателей репродуктивной системы отмечается у самцов, рожденных от матерей с СД: по сравнению с потом-ством от матерей без СД у них ниже уровень гонадотропных гор-монов и тестостерона крови, а в гонадах меньше на 15,6% клеток Лейдига и на 14,9% клеток Сертоли, количество сперматогоний снижено на 26,3%, а диаметр семенных канальцев -на 11% [40].…”

Section: экспериментальные данныеunclassified