Endocytic membrane trafficking in the control of centrosome function

Naslavsky, Naava; Caplan, Steve

doi:10.1016/j.ceb.2020.01.009

Cited by 24 publications

(25 citation statements)

References 40 publications

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“…Cells internalize extracellular signaling molecules through endocytosis, and endocytosed proteins are either recycled back to the plasma membrane or transported to lysosomes for degradation [30][31][32]. Endosomal transportation is largely regulated by the Ras-related in brain (RAB) family of small GTPases, which contains over 60 members with varying tissue distribution; individual RAB GTPases coordinate distinct steps in anterograde and retrograde protein trafficking [58,59].…”

Section: Discussionmentioning

confidence: 99%

“…RAB11FIP5 plays an important role in endosomal recycling by recycling proteins back to the plasma membrane or transporting proteins to lysosomes for degradation [30][31][32]35]. To determine whether RAB11FIP5 induces ORF45 degradation by promoting the translocation of ORF45 to lysosomes, we isolated lysosomes from HEK293T cells transfected with ORF45 alone or cotransfected with ORF45 and RAB11FIP5; both groups of cells were treated with CHLO to inhibit ORF45 protein degradation.…”

Section: Plos Pathogensmentioning

confidence: 99%

“…Endocytosed proteins are either recycled back to the plasma membrane or transported to lysosomes for degradation [30][31][32]. The RAB11 GTPase has emerged as an important regulator of endocytic transport [33].…”

Section: Introductionmentioning

confidence: 99%

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Host RAB11FIP5 protein inhibits the release of Kaposi’s sarcoma-associated herpesvirus particles by promoting lysosomal degradation of ORF45

et al. 2020

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Open reading frame (ORF) 45 is an outer tegument protein of Kaposi’s sarcoma-associated herpesvirus (KSHV). Genetic analysis of an ORF45-null mutant revealed that ORF45 plays a key role in the events leading to the release of KSHV particles. ORF45 associates with lipid rafts (LRs), which is responsible for the colocalization of viral particles with the trans-Golgi network and facilitates their release. In this study, we identified a host protein, RAB11 family interacting protein 5 (RAB11FIP5), that interacts with ORF45 in vitro and in vivo. RAB11FIP5 encodes a RAB11 effector protein that regulates endosomal trafficking. Overexpression of RAB11FIP5 in KSHV-infected cells decreased the expression level of ORF45 and inhibited the release of KSHV particles, as reflected by the significant reduction in the number of extracellular virions. In contrast, silencing endogenous RAB11FIP5 increased ORF45 expression and promoted the release of KSHV particles. We further showed that RAB11FIP5 mediates lysosomal degradation of ORF45, which impairs its ability to target LRs in the Golgi apparatus and inhibits ORF45-mediated colocalization of viral particles with the trans-Golgi network. Collectively, our results suggest that RAB11FIP5 enhances lysosome-dependent degradation of ORF45, which inhibits the release of KSHV particles, and have potential implications for virology and antiviral design.

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Section: Discussionmentioning

confidence: 99%

Section: Plos Pathogensmentioning

confidence: 99%

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Host RAB11FIP5 protein inhibits the release of Kaposi’s sarcoma-associated herpesvirus particles by promoting lysosomal degradation of ORF45

et al. 2020

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“…Article centrosomal function (Naslavsky and Caplan, 2020). To our knowledge, there are no reports of a membrane-independent role of Rab5 although other groups have reported examples of trafficking proteins involved in MT nucleation in a membrane-independent manner, such as ALIX, a PCM component in human and fly cells, whose recruitment depends on Cnn/Cep215 and D-Spd2/Cep192 (Malerød et al, 2018).…”

Section: Accessmentioning

confidence: 99%

Mauve/LYST limits fusion of lysosome-related organelles and promotes centrosomal recruitment of microtubule nucleating proteins

et al. 2021

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Summary Lysosome-related organelles (LROs) are endosomal compartments carrying tissue-specific proteins, which become enlarged in Chediak-Higashi syndrome (CHS) due to mutations in LYST . Here, we show that Drosophila Mauve, a counterpart of LYST , suppresses vesicle fusion events with lipid droplets (LDs) during the formation of yolk granules (YGs), the LROs of the syncytial embryo, and opposes Rab5, which promotes fusion. Mauve localizes on YGs and at spindle poles, and it co-immunoprecipitates with the LDs’ component and microtubule-associated protein Minispindles/Ch-TOG. Minispindles levels are increased at the enlarged YGs and diminished around centrosomes in mauve -derived mutant embryos. This leads to decreased microtubule nucleation from centrosomes, a defect that can be rescued by dominant-negative Rab5. Together, this reveals an unanticipated link between endosomal vesicles and centrosomes. These findings establish Mauve/LYST’s role in regulating LRO formation and centrosome behavior, a role that could account for the enlarged LROs and centrosome positioning defects at the immune synapse of CHS patients.

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“…The review highlights how these endocytic pathways coordinate with secretory traffic to deploy proteins to specific membrane domains in a highly targeted fashion. Continuing on the theme of highly targeted traffic, Naslavsky and Caplan [23] review endocytic traffic that helps to regulate organelles without membranes, such as cilia and the centrosome, which are supported by endocytic machinery throughout the cell cycle. The review by Noack and Mukherjee [6] highlights recent examples of sorting and trafficking events that are highjacked by bacterial and viral pathogens, including mechanisms for subverting traffic at the level of the endoplasmic reticulum and in mitophagy.…”

Section: Jennifer L Stowmentioning

confidence: 99%

Editorial overview: Membrane traffic in the time of COVID-19

Brodsky

Stow

2020

Current Opinion in Cell Biology

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Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre-including this research content-immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

show abstract

Endocytic membrane trafficking in the control of centrosome function

Cited by 24 publications

References 40 publications

Host RAB11FIP5 protein inhibits the release of Kaposi’s sarcoma-associated herpesvirus particles by promoting lysosomal degradation of ORF45

Host RAB11FIP5 protein inhibits the release of Kaposi’s sarcoma-associated herpesvirus particles by promoting lysosomal degradation of ORF45

Mauve/LYST limits fusion of lysosome-related organelles and promotes centrosomal recruitment of microtubule nucleating proteins

Editorial overview: Membrane traffic in the time of COVID-19

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