Endocytosis and exocytosis of nanoparticles in mammalian cells

Oh, Nuri; Park, Ji-Ho

doi:10.2147/ijn.s26592

Cited by 534 publications

(221 citation statements)

References 48 publications

Supporting

Mentioning

205

Contrasting

Unclassified

Order By: Relevance

“…In clathrin-independent endocytosis (CME) or synonymously, receptor-mediated endocytosis (RME), the cargoes are wrapped up into the clathrin-coated pits by curvature of plasma membrane, the structure fuses into clathrin-coated vesicles which subsequently detached from the plasma membrane, the contents are thus being delivered into the cells. Classification of cellular uptake mechanisms seems to be varied according to different views (Doherty and McMahon 2009;Iversen et al 2011;Oh and Park 2014). According to Doherty and McMahon (2009), phagocytosis which occurs specifically in monocytes, neutrophils, dendritic cells and macrophages is classified as CIE, along with the clathrin-independent carriers/GPI-AP enriched early endosomal compartment (CLIC/GEEC) pathway, macropinocytosis, and other pathways which have been characterized in the very recent decades (Doherty and McMahon 2009).…”

Section: Molecular Mechanisms Of Swnts Nanotoxicitymentioning

confidence: 99%

Toxicity of single-walled carbon nanotubes

et al. 2014

View full text Add to dashboard Cite

Carbon nanotubes (CNTs) are an important class of nanomaterials, which have numerous novel properties that make them useful in technology and industry. Generally, there are two types of CNTs: single-walled nanotubes (SWNTs) and multi-walled nanotubes. SWNTs, in particular, possess unique electrical, mechanical, and thermal properties, allowing for a wide range of applications in various fields, including the electronic, computer, aerospace, and biomedical industries. However, the use of SWNTs has come under scrutiny, not only due to their peculiar nanotoxicological profile, but also due to the forecasted increase in SWNT production in the near future. As such, the risk of human exposure is likely to be increased substantially. Yet, our understanding of the toxicological risk of SWNTs in human biology remains limited. This review seeks to examine representative data on the nanotoxicity of SWNTs by first considering how SWNTs are absorbed, distributed, accumulated and excreted in a biological system, and how SWNTs induce organ-specific toxicity in the body. The contradictory findings of numerous studies with regards to the potential hazards of SWNT exposure are discussed in this review. The possible mechanisms and molecular pathways associated with SWNT nanotoxicity in target organs and specific cell types are presented. We hope that this review will stimulate further research into the fundamental aspects of CNTs, especially the biological interactions which arise due to the unique intrinsic characteristics of CNTs.

show abstract

Section: Molecular Mechanisms Of Swnts Nanotoxicitymentioning

confidence: 99%

Toxicity of single-walled carbon nanotubes

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Following endocytosis of FRB-VC loaded MSNs (MSN-FRB-VC)[44], carrier and cargo proceed through the endosomal system where decreasing pH causes dissociation of His-tagged FRB-VC from the MSN. Endosomal rupture is triggered using chloroquine[19] and FRB-VC is released to the cytosol.…”

Section: Resultsmentioning

confidence: 99%

Nanoparticle mediated delivery and small molecule triggered activation of proteins in the nucleus

Chiu

Bates

Helma

et al. 2018

Nucleus

View full text Add to dashboard Cite

Protein transfection is a versatile tool to study or manipulate cellular processes and also shows great therapeutic potential. However, the repertoire of cost effective techniques for efficient and minimally cytotoxic delivery remains limited. Mesoporous silica nanoparticles (MSNs) are multifunctional nanocarriers for cellular delivery of a wide range of molecules, they are simple and economical to synthesize and have shown great promise for protein delivery. In this work we present a general strategy to optimize the delivery of active protein to the nucleus. We generated a bimolecular Venus based optical sensor that exclusively detects active and bioavailable protein for the performance of multi-parameter optimization of protein delivery. In conjunction with cell viability tests we maximized MSN protein delivery and biocompatibility and achieved highly efficient protein transfection rates of 80%. Using the sensor to measure live-cell protein delivery kinetics, we observed heterogeneous timings within cell populations which could have a confounding effect on function studies. To address this problem we fused a split or dimerization dependent protein of interest to chemically induced dimerization (CID) components, permitting control over its activity following cellular delivery. Using the split Venus protein we directly show that addition of a small molecule dimerizer causes synchronous activation of the delivered protein across the entire cell population. This combination of cellular delivery and triggered activation provides a defined starting point for functional studies and could be applied to other protein transfection methods.

show abstract

“…The speed of exocytosis has also been reported to be dependent on nanoparticle size. 105 In a study by Xu et al 106 larger PLGAencapsulated iron oxide nanoparticles had a 3-fold longer retention in mesenchymal stem cells compared with smaller iron oxide nanoparticles. Active exocytosis has also been reported for various other types of nanoparticles [107][108][109] and can occur via lysosome secretion, vesicle-related secretion and non-vesiclerelated secretion.…”

Section: Effects Of Nanoparticle Sizementioning

confidence: 99%

“…Active exocytosis has also been reported for various other types of nanoparticles [107][108][109] and can occur via lysosome secretion, vesicle-related secretion and non-vesiclerelated secretion. 105 Release of nanoparticles either by active exocytosis or following cell death is considered one of the main disadvantages of the use of nanoparticles for cell labelling and imaging. The released nanoparticles can reside for a long time or be taken up by other cells and thus create a contrast agentrelated signal that is not related to the presence of the transplanted cell itself.…”

Section: Effects Of Nanoparticle Sizementioning

confidence: 99%

Nanoparticles and clinically applicable cell tracking

Bernsen

Guenoun

Tiel

et al. 2015

BJR

View full text Add to dashboard Cite

In vivo cell tracking has emerged as a much sought after tool for design and monitoring of cell-based treatment strategies. Various techniques are available for pre-clinical animal studies, from which much has been learned and still can be learned. However, there is also a need for clinically translatable techniques. Central to in vivo cell imaging is labelling of cells with agents that can give rise to signals in vivo, that can be detected and measured non-invasively. The current imaging technology of choice for clinical translation is MRI in combination with labelling of cells with magnetic agents. The main challenge encountered during the cell labelling procedure is to efficiently incorporate the label into the cell, such that the labelled cells can be imaged at high sensitivity for prolonged periods of time, without the labelling process affecting the functionality of the cells. In this respect, nanoparticles offer attractive features since their structure and chemical properties can be modified to facilitate cellular incorporation and because they can carry a high payload of the relevant label into cells. While these technologies have already been applied in clinical trials and have increased the understanding of cell-based therapy mechanism, many challenges are still faced.

show abstract

Endocytosis and exocytosis of nanoparticles in mammalian cells

Cited by 534 publications

References 48 publications

Toxicity of single-walled carbon nanotubes

Toxicity of single-walled carbon nanotubes

Nanoparticle mediated delivery and small molecule triggered activation of proteins in the nucleus

Nanoparticles and clinically applicable cell tracking

Contact Info

Product

Resources

About