Endocytosis of Multiwalled Carbon Nanotubes in Bronchial Epithelial and Mesothelial Cells

Maruyama, Kayo; Haniu, Hisao; Saito, Naoto; Matsuda, Yoshikazu; Tsukahara, Tamotsu; Kobayashi, Shinsuke; Takanashi, Seiji; Okamoto, Masato; Kato, Hiroyuki

doi:10.1155/2015/793186

Cited by 57 publications

(46 citation statements)

References 49 publications

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“…Although MWCNTs dispersed with gelatin and DPPC induce significant cytotoxicity, carboxymethyl cellulose (CMC) does not show cytotoxicity in BEAS-2B cells. Moreover, we compared the cytotoxicity of gelatin with FBS as a dispersant for MWNT-7 on normal human bronchial epithelial cells, and FBS showed lower cytotoxicity than gelatin [34]. Liu et al also reported that single-walled CNT coated with albumin, the most abundant protein in serum, decreased cytotoxicity by reducing cellular uptake [25].…”

Section: Discussionmentioning

confidence: 99%

The Dispersion State of Tangled Multi-Walled Carbon Nanotubes Affects Their Cytotoxicity

et al. 2016

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The medical applications of carbon nanotubes (CNTs) have garnered much attention. However, evaluating the safety of CNTs remains difficult, and no consensus has been reached. Moreover, assessing the biosafety of multi-walled CNTs (MWCNTs), which can become tangled during manufacturing, is challenging because they do not readily disperse. We studied how the dispersion state of tangled MWCNTs affects their cytotoxicity, using three sonicators. Flotube 9110 (FT9110), tangled MWCNTs, were dispersed in two dispersants (fetal bovine serum and polysorbate 80) using a new type of sonicator (PR-1) and two conventional sonicators. The size and cytotoxicity of the dispersed FT9110 were measured using the BEAS-2B human bronchial epithelial cell line. The PR-1 dispersed the FT9110 to agglomerates <200 nm in diameter; FT9110 dispersed with the PR-1 did not show cytotoxicity regardless of dispersant. The other sonicators dispersed the FT9110 to particles >1000 nm in diameter, and cytotoxicity depended on the dispersant. We found that excluding cells adhered to agglomerated FT9110 before evaluating cytotoxicity can lead to false-positive results. The PR-1 sonicator dispersed tangled FT9110 to many single fibers, which showed lower cytotoxicity than conventionally-sonicated MWCNTs. We suggest that dispersion state should be accounted for when evaluating the cytotoxicity of MWCNTs.

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Section: Discussionmentioning

confidence: 99%

The Dispersion State of Tangled Multi-Walled Carbon Nanotubes Affects Their Cytotoxicity

et al. 2016

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Section: Carbon Nanoparticlesmentioning

confidence: 98%

“…Clathrin mediated endocytosis inhibitors significantly suppressed MWCNT uptake, whereas caveolae mediated endocytosis and macropinocytosis were also found to be involved in MWCNT uptake. Thus, MWCNTs were positively taken up by nonphagocytic cells, and their cytotoxicity was closely related to these three endocytosis pathways [57].Chen GY demonstrated that GO treatment of cells simultaneously triggers autophagy and TLR4/TLR9-regulated inflammatory responses, and the autophagy was at least partly regulated by the TLRs pathway, suggesting a mechanism by which cells respond to nanomaterials and underscores the importance of future safety evaluation of nanomaterials [32].Citation: Liu Y, Wang J, Jiang M, Tong X, Han C, et al (2017) The Role of Cell Autophagy in Toxicity Caused by Dental Nanomaterials. J Nanotechnol Nanomed Nanobiotechnol 4: 011.…”

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confidence: 99%

The Role of Cell Autophagy in Toxicity Caused by Dental Nanomaterials

Wei¹

2017

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NanomaterialsThe overview of nanomaterials Nanomaterials are defined as materials composed of unbound particles or particles in an aggregate or agglomerate state with one or more external dimensions with a size ranging from 1nm to 100nm [1]. Nanomaterials have a lot of free surfaces and interfaces and there is interaction between the various units. Based on the above characteristics, nanomaterials are featured by surface effect, small size effect, quantum size effect, macroscopic quantum tunneling effect and the dielectric confinement effect. Nanoparticles have lots of unpaired atoms, large specific surface area, less surface defects and can occur in conjunction with the polymer stronger physically or chemically. Added to the polymer material, they can improve the ductility, toughness, strength, barrier properties, heat resistance and dimensional stability of the material. They have been widely used in conventional materials, medical equipment, electronic equipment, paint and other industries. However, nanomaterials will affect the organism and produce a series of toxic effects by inducing oxidative stress and inflammatory response mechanisms in the cell, subcellular and protein levels. The nanomaterials commonly used in dentistryNanometer materials are widely applied in the field of dentistry, including dental restoration, antibacterial, caries treatment, orthodontic, root canal, bonding systems and so on.Adding nanoparticles to dental restoration materials to improve the performance has been widely used in the clinical field. In view of its unpaired atoms, large specific surface area, less surface defects and occurring in conjunction with the polymer either stronger physically or chemically, the novel resin composite exhibited a strong antibacterial property upon the addition of up to 5% nano antibacterial inorganic fillers, there by leading to effective caries inhibition in dental application [2]. The mesoporous silica biomaterials have great potential for serving as both a catalyst and carrier in the repair or regeneration of dental hard tissue [3]. Diatomite-based nanocomposite ceramics are good potential candidates for ceramic-based dental materials [4]. Nano silicon dioxide can also give polymers the characteristics of better viscosity, thermal stability, high strength, less volume shrinkage and of course, durability [5].In clinical endodontics especially root canal and restorative treatments we can see the near future potential of nanoparticles from the application of nanoparticles in the form of solutions for irrigation, medication and as an additive within sealers/restorative materials [6]. GICs can be applied to base plates of Orthodontic and modified denture base well, as which contain chlorhexidine hexametaphosphate nanoparticles and characterize the nanoparticle size shows a stronger antimicrobial activity [7].As for caries treatment, Hannig M said that Analysis of in vitro data indicates that apatite nanoparticles might be effective in reversing lesion progression in the outer but not in the deepe...

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“…in 2018 have shown that the oxidized SWCNTs enter S. cerevisiae cells via endocytosis, and successfully accumulates into various cellular compartments like cytoplasm, vesicles, lysosomes, and the cell nucleus. Maruyama et al . in 2015 reported their efforts on the study of MWCNTs penetration in normal human bronchial epithelial (HBECs) and mesothelium (HMCs) cells.…”

Section: Introductionmentioning

confidence: 99%

Derivatized Carbon Nanotubes for Gene Therapy in Mammalian and Plant Cells

et al. 2020

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The concept of gene vectors for therapeutic applications has been known for several years, but it is far from revealing its actual potential. With the advent of hollow cylindrical carbon nanomaterials such as carbon nanotubes (CNTs), researchers have invented several new tools to deliver genes at the required site of action in mammalian and plant cells. The ease of diversified functionalization has allowed CNTs to be by far the most adaptable non‐viral vector for gene therapy. This Minireview addresses the dexterity with which CNTs undergo surface modifications and their applications as a potent vector in gene therapy of humans and plants. Specifically, we will discuss the new tools that scientific communities have invented to achieve gene therapy using plasmid DNA, RNA silencing, suicide gene therapy, and plant genetic engineering. Additionally, we will shed some light on the mechanism of gene transportation using carbon nanotubes in cancer cells and plants.

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Endocytosis of Multiwalled Carbon Nanotubes in Bronchial Epithelial and Mesothelial Cells

Cited by 57 publications

References 49 publications

The Dispersion State of Tangled Multi-Walled Carbon Nanotubes Affects Their Cytotoxicity

The Dispersion State of Tangled Multi-Walled Carbon Nanotubes Affects Their Cytotoxicity

The Role of Cell Autophagy in Toxicity Caused by Dental Nanomaterials

Derivatized Carbon Nanotubes for Gene Therapy in Mammalian and Plant Cells

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