Endogenous Erythroid Colony Formation by Peripheral Blood Mononuclear Cells from Patients with Myelofibrosis and Polycythemia Vera

Lutton, John D.; Levere, Richard D.

doi:10.1159/000207547

Cited by 72 publications

(30 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Spontaneous colony formation of erythroid colonies has been well described in polycythemia vera and MMM 25,26 and has led to the evaluation of spontaneous megakaryocyte colony formation in ET. 27 The spontaneous growth of megakaryocyte colonies, in the absence of the prerequisite cytokine TPO, has been described as a specific method for distinguishing ET from RT.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic and prognostic value of bone marrow angiogenesis and megakaryocyte c-Mpl expression in essential thrombocythemia

Mesa

Hanson

et al. 2002

Blood

View full text Add to dashboard Cite

The lack of diagnostic certainty in some patients makes it difficult to distinguish between primary and secondary forms of thrombocytosis. To augment current diagnostic studies for thrombocytosis, we retrospectively evaluated clinical records and bone marrow trephine specimens of 183 patients with thrombocytosis-164 with essential thrombocythemia (ET), 19 with reactive thrombocytosis (RT)-for bone marrow angiogenesis, bone marrow megakaryocyte c-Mpl staining, and morphologic evidence of megakaryocyte proliferation. Angiogenesis was increased in patients with ET compared with healthy controls (P < .0001) and patients with RT (P ‫؍‬ .006). In addition, an increase in angiogenesis was associated with certain disease features such as splenomegaly (P ‫؍‬ .004) and reticulin fibrosis (P ‫؍‬ .005). Decreased megakaryocyte c-Mpl staining was observed in a heterogeneous pattern in ET compared with healthy controls (P < .0001) and RT (P < .0001). Histologic stratifying criteria incorporating increased angiogenesis, decreased megakaryocyte c-Mpl expression, and marked megakaryocyte proliferation in the bone marrow was highly sensitive (97%) and specific (95%) for distinguishing ET from RT (P < .0001

show abstract

Section: Discussionmentioning

confidence: 99%

Diagnostic and prognostic value of bone marrow angiogenesis and megakaryocyte c-Mpl expression in essential thrombocythemia

Mesa

Hanson

et al. 2002

Blood

View full text Add to dashboard Cite

show abstract

“…At about the same time Jaroslav Prchal and Arthur Axelrad 10 demonstrated that erythroid colonies could be grown from PV bone marrow cells, but not from normal cells, in the absence of erythropoietin; this has been termed endogenous erythroid colony formation, and can be demonstrated in some, but not all, patients with ET and PMF. 10,11 In subsequent years, our understanding of the molecular basis of PV, ET and PMF remained unchanged, with the notable exception of two papers in 1998; when Silva et al 12 noted overexpression of BCL-X L in PV erythroid progenitors, and Moliterno et al 13 noted downmodulation of thrombopoietin receptor expression on PV platelets.…”

Section: Introductionmentioning

confidence: 99%

JAK2 and MPL mutations in myeloproliferative neoplasms: discovery and science

2008

View full text Add to dashboard Cite

Although it has long been known that the myeloproliferative neoplasms (MPN) polycythemia vera (PV), essential thrombocythemia (ET) and primary myelofibrosis (PMF) are clonal hematopoietic stem-cell disorders, for many years the genetic basis for these disorders was elusive. A new era in MPN biology began in 2005 with the discovery of a somatic point mutation in JAK2 tyrosine kinase (JAK2V617F), which was identified in a significant proportion of patients with PV, ET and PMF. Based on the hypothesis that JAK-STAT signaling is central to the pathogenesis of JAK2V617F-negative MPN, genomic studies have identified JAK2 exon 12 mutations in JAK2V617F-negative PV and activating mutations in MPL in patients with JAK2V617F-negative ET and PMF. In this review, we will discuss the role of these mutant alleles in the pathogenesis of PV, ET and PMF, the potential therapeutic implications of these discoveries, and the implications of these discoveries for genomic studies of hematopoietic malignancies.

show abstract

“…In this respect, progeni tor cells from PV and myelofibrosis have been shown to generate significant numbers of Epo-independent endogenous megakaryocyte and erythroid colonies [3,4].…”

Section: Discussionmentioning

confidence: 99%

Humoral-Dependent Hemopoiesis and Flow Cytometric Analysis of Chronic Myelogenous Leukemia in Erythroblastic Transformation

Lutton¹,

CHIAO²,

Ascensāo³

et al. 1987

Acta Haematol

View full text Add to dashboard Cite

Bone marrow and peripheral blood cells from a patient with chronic myelogenous leukemia in erythroblastic transformation were studied by flow cytometry and for hemopoietic colony growth. Results demonstrated that this disorder had greatly expanded bone marrow erythroid colony (CFU-E) and myeloid colony (CFU-GM) progenitor compartments that were totally dependent upon erythropoietin and colony-stimulating factor. DNA, RNA and cell cycle analysis revealed that the bone marrow cells were diploid, had a high percentage of S phase cells (17%), and a unique bimodal RNA index of 5 and 13.8. Results are discussed and contrasted with other myeloproliferative disorders.

show abstract

Endogenous Erythroid Colony Formation by Peripheral Blood Mononuclear Cells from Patients with Myelofibrosis and Polycythemia Vera

Cited by 72 publications

References 6 publications

Diagnostic and prognostic value of bone marrow angiogenesis and megakaryocyte c-Mpl expression in essential thrombocythemia

Diagnostic and prognostic value of bone marrow angiogenesis and megakaryocyte c-Mpl expression in essential thrombocythemia

JAK2 and MPL mutations in myeloproliferative neoplasms: discovery and science

Humoral-Dependent Hemopoiesis and Flow Cytometric Analysis of Chronic Myelogenous Leukemia in Erythroblastic Transformation

Contact Info

Product

Resources

About