Endogenous microbial contamination of cultured autologous preparations in trials of cancer immunotherapy

Influence of harvest bacterial contamination on autologous peripheral blood progenitor cells post-transplant

“…Five hundred and five non-contaminated bags were administered to 135 patients (median 3; range: [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. In the group of 17 patients receiving contaminated PBPCs, a total of 71 bags were administered (the 31 contaminated ones referred to above, plus 40 uncontaminated bags) (median 5; range: 1-9); a median of 1 contaminated bag per patient (range 1-5).…”

“…[1][2][3][4] Microbial contamination has also been reported in blood processed for immunotherapy 5 and in hematopoietic progenitor cell transplants. In these cases, the contamination may be, according to the source of stem cells, from 0 to 4.5% in peripheral blood to as high as 26% in bone marrow.…”

“…In these cases, the contamination may be, according to the source of stem cells, from 0 to 4.5% in peripheral blood to as high as 26% in bone marrow. [5][6][7][8][9][10] Peripheral blood progenitor cells (PBPCs) have been increasingly used for both autologous and allogeneic transplants. In autologous PBPC transplants (APBPCT), cells are obtained frequently by apheresis through a central venous line, but this is also known to be a possible route for infection.…”

Microbial contamination of hematopoietic progenitor cell products: clinical outcome

“…Microbial contamination of HPC products has been described in frequencies varying from close to 1% up to high contamination rates. [1][2][3][4] Harvesting, ex vivo processing, cryopreservation and the pre-infusion thawing process [5][6][7] can be responsible for contamination, owing to inadequate decontamination of skin at the needle puncture site, indwelling catheter site for peripheral blood progenitor cell (PBPC) collection or owing to contamination by laboratory personnel or equipment. Commonly documented species are part of the normal flora of the skin.…”

Cryopreservation of Hematopoietic and Non-Hematopoietic Stem Cells – A Review for the Clinician

“…Infectious contaminations during those processes are observed with varying degrees, generally 0-4.5% for PBSC and up to 26% for bone marrow, even if strictly aseptic protocols are followed (Attarian, Bensinger, Buckner, D. L. McDonald, & Rowley, 1996;Espinosa, Fox, Creger, & Lazarus, 1996;Jestice et al, 1996;Kamble et al, 2005;Larrea et al, 2004;Lowder & Whelton, 2003;Majado et al, 2007;D. J. Padley et al, 2003;D.…”