Endogenous miRNA Sponges

Alkan, Ayşe Hale; Akgül, Bünyamin

doi:10.1007/978-1-0716-1170-8_5

Cited by 67 publications

(37 citation statements)

References 107 publications

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“…It has been demonstrated that lncRNAs function as miRNA “sponges”, which competitively suppress the activity of miRNAs ( Alkan and Akgül, 2022 ). Since lncRNAs interact with miRNAs through miRNA Response Elements (MREs), combined using the free energy and score of RNAhybrid, miRanda and TargetScan databases, the potential MREs were predicted and 38 DEmiRNAs that putatively targeted 8 DElncRNAs were then identified ( Supplementary Table S6 ).…”

Section: Resultsmentioning

confidence: 99%

Integrative Analysis of RNA Expression and Regulatory Networks in Mice Liver Infected by Echinococcus multilocularis

Liu

et al. 2022

Front. Cell Dev. Biol.

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The larvae of Echinococcus multilocularis causes alveolar echinococcosis, which poses a great threat to the public health. However, the molecular mechanisms underlying the host and parasite interactions are still unclear. Exploring the transcriptomic maps of mRNA, miRNA and lncRNA expressed in the liver in response to E. multilocularis infection will help us to understand its pathogenesis. Using liver perfusion, different cell populations including the hepatic cells, hepatic stellate cells and Kupffer cells were isolated from mice interperitoneally inoculated with protoscoleces. Their transcriptional profiles including lncRNAs, miRNAs and mRNAs were done by RNA-seq. Among these cell populations, the most differentially-expressed (DE) mRNA, lncRNAs and miRNAs were annotated and may involve in the pathological processes, mainly including metabolic disorders, immune responses and liver fibrosis. Following the integrative analysis of 38 differentially-expressed DEmiRNAs and 8 DElncRNAs, the lncRNA-mRNA-miRNA networks were constructed, including F63-miR-223-3p-Fbxw7/ZFP36/map1b, F63-miR-27-5p-Tdrd6/Dip2c/Wdfy4 and IFNgAS1-IFN-γ. These results unveil the presence of several potential lncRNA-mRNA-miRNA axes during E. multilocularis infection, and further exploring of these axes may contribute to better understanding of the pathogenic mechanisms.

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Section: Resultsmentioning

confidence: 99%

Integrative Analysis of RNA Expression and Regulatory Networks in Mice Liver Infected by Echinococcus multilocularis

Liu

et al. 2022

Front. Cell Dev. Biol.

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“…Among these abnormally expressed miRNAs, we identified miR-181d-5p to play a critical role in RGCs death in TON. According to previous reports, miRNAs are involved in gene regulation and other cellular processes and play a wide range of roles [ 23 ], including RGCs death [ 31 ]. Here, we found that miR-181d-5p targets the NFIA gene.…”

Section: Discussionmentioning

confidence: 99%

“…Retinal Tissues. MicroRNAs (miRNAs) can be involved in the pathophysiologic process of many diseases by binding to target mRNAs [23]. A recent study identified that ocular hypertension and TON induce significant changes in RGCs miRNAs [24].…”

Section: Mir-181d-5p Is Expressed At Low Levels In Injuredmentioning

confidence: 99%

miR-181d-5p Protects against Retinal Ganglion Cell Death after Blunt Ocular Injury by Regulating NFIA-Medicated Astrocyte Development

Liu

Yuan

et al. 2022

Mediators of Inflammation

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Background. Traumatic optic neuropathy (TON) refers to damage to the optic nerve resulting from direct and indirect trauma to the head and face. One of the important pathological processes in TON is the death of retinal ganglion cells (RGCs), but the cause of RGCs death remains unclear. We aimed to explore the mechanisms of RGCs death in an experimental TON model. Methods. Optic nerve crush injury was induced in ten New Zealand white rabbits. On the 1st, 3rd, 7th, 14th, and 28th days after the operation, the retinal tissues of the rabbits were observed pathologically by hematoxylin-eosin staining. The expression of POU-homeodomain transcription factor Brn3a and glial fibrillary acidic protein (GFAP) was measured by immunofluorescence to evaluate the number of RGCs and astrocytes, respectively. miRNA expression and protein levels were assessed by RT-qPCR and western blot methods, respectively. Finally, the malondialdehyde content, superoxide dismutase activity, and proinflammatory factor levels were measured by ELISA. Western blot and dual-luciferase reporter assays were used to elucidate the relationship between miR-181d-5p and nuclear factor I-A (NFIA). Results. Blunt ocular trauma increased oxidative stress and apoptosis and reduced ganglion cell layer (GCL) density. The expression of miR-181d-5p was decreased in retinal tissues, and its overexpression relieved RGCs death, astrocyte development, oxidative stress, and inflammation of the retina, which were reversed by NFIA overexpression. Conclusion. miR-181d-5p can protect against the deterioration of TON by inhibiting RGCs death, astrocyte development, oxidative stress, and inflammation by targeting NFIA. This study provides new insight into early medical intervention in patients with TON.

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“…Plentiful circRNAs have demonstrated to regulate multiple biological processes of PTC cells, such as survival, apoptosis, invasion, and metastasis 8–10 . Increasing studies report that circRNA is a crucial member of competitive endogenous RNA (ceRNA), and acts as a miRNA “sponge” to relieve the inhibitory effect on its target genes 11,12 . Lv et al have used RNA deep sequencing to determine the expression patterns of circRNAs in PTC tumors and discovered that circPTPRM (circBase ID: hsa_circ_0007144) is highly expressed in PTC patients 13 .…”

Section: Introductionmentioning

confidence: 99%

“…[8][9][10] Increasing studies report that circRNA is a crucial member of competitive endogenous RNA (ceRNA), and acts as a miRNA "sponge" to relieve the inhibitory effect on its target genes. 11,12 Lv et al have used RNA deep sequencing to determine the expression patterns of circRNAs in PTC tumors and discovered that circPTPRM (circBase ID: hsa_circ_0007144) is highly expressed in PTC patients. 13 However, the precise roles of circPTPRM in the development of PTC and its associated ceRNA network remain to be further clarified.…”

mentioning

confidence: 99%

CircPTPRM accelerates malignancy of papillary thyroid cancer via miR‐885‐5p/DNMT3A axis

Chen

Jiang

Luo

et al. 2022

Clinical Laboratory Analysis

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Background Circular RNAs (circRNAs) are implicated in carcinogenesis, including papillary thyroid cancer (PTC). Despite of previous reports regarding the high expression of circPTPRM in PTC, the role and regulatory mechanism remain to be investigated. Methods CircPTPRM and miR‐885‐5p expression were examined, and the effects on cell proliferation, migration, and invasion were also measured. Immunoblotting was performed to evaluate DNA methyltransferase 3A (DNMT3A) and the epithelial‐mesenchymal transition (EMT)‐associated proteins. Results CircPTPRM was overexpressed in PTC tissues and cell lines, which predicted poor prognosis. CircPTPRM inhibition significantly alleviated the proliferation, migration, and invasion abilities. It was subsequently confirmed that circPTPRM competed with miR‐885‐5p for DNMT3A binding. CircPTPRM promoted PTC progression via miR‐885‐5p/DNMT3A signal axis. Conclusion Our data elucidated that circPTPRM may play an oncogenic role in PTC through circPTPRM/miR‐885‐5p/DNMT3A axis

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Endogenous miRNA Sponges

Cited by 67 publications

References 107 publications

Integrative Analysis of RNA Expression and Regulatory Networks in Mice Liver Infected by Echinococcus multilocularis

Integrative Analysis of RNA Expression and Regulatory Networks in Mice Liver Infected by Echinococcus multilocularis

miR-181d-5p Protects against Retinal Ganglion Cell Death after Blunt Ocular Injury by Regulating NFIA-Medicated Astrocyte Development

CircPTPRM accelerates malignancy of papillary thyroid cancer via miR‐885‐5p/DNMT3A axis

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