1995
DOI: 10.1073/pnas.92.12.5754
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Endogenous mutagenesis by an insertion sequence element identifies Aeromonas salmonicida AbcA as an ATP-binding cassette transport protein required for biogenesis of smooth lipopolysaccharide.

Abstract: Analysis of an Aeromonas salmonicida A lay-

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Cited by 24 publications
(30 citation statements)
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“…For example, the rfb region of some K-12 strains contains a mutation resulting from an IS5 element which interrupts the last gene of the cluster, the putative rhamnose transferase, and prevents O-antigen synthesis (42). An IS element (ISAS1) is also responsible for mutation to O-antigen deficiency in some isolates of Aeromonas salmonicida (8). An IS element has also been found within the rfb gene cluster of Pseudomonas aeruginosa O5, although it does not inactivate O-antigen expression (7).…”
Section: Resultsmentioning
confidence: 99%
“…For example, the rfb region of some K-12 strains contains a mutation resulting from an IS5 element which interrupts the last gene of the cluster, the putative rhamnose transferase, and prevents O-antigen synthesis (42). An IS element (ISAS1) is also responsible for mutation to O-antigen deficiency in some isolates of Aeromonas salmonicida (8). An IS element has also been found within the rfb gene cluster of Pseudomonas aeruginosa O5, although it does not inactivate O-antigen expression (7).…”
Section: Resultsmentioning
confidence: 99%
“…In one extreme example, the Aeromonas salmonicida O polysaccharides have a uniform chain length (322). The observation that modality is conserved when O polysaccharides are expressed from cloned genes in heterologous hosts (i.e., E. coli K-12) demonstrates that modal regulation is a function of one or more of the components encoded by the biosynthesis cluster itself.…”
mentioning
confidence: 97%
“…This bacterium produces homogenous (nonmodal) O-PS chain lengths (22), and the NBD protein (AbcA) is required for O-PS export (24). AbcA also contains an extended C-terminal domain that contains a putative leucine zipper, which is not essential for O-PS export but is required for proper transcription of the surface layer protein gene vapA (25,100).…”
Section: Variations On the Theme-diversity In Function Of Extended C-mentioning
confidence: 99%