2002
DOI: 10.1097/00005344-200202000-00005
|View full text |Cite
|
Sign up to set email alerts
|

Endogenous Nitric Oxide Synthase Inhibitors in Endothelial Cells, Endothelin-1 Within the Vessel Wall, and Intimal Hyperplasia in Perimenopausal Human Uterine Arteries

Abstract: The present experiments were designed to investigate the ability to produce nitric oxide, concentrations of N(G)-monomethyl-L-arginine (L-NMMA), and asymmetric N(G), N(G)-dimethyl-L-arginine (ADMA) in endothelial cells, endothelin-1 within the vessel wall, and the degree of intimal hyperplasia (intima/media ratio) in perimenopausal human uterine arteries. According to the tentative classification based on basal cyclic GMP levels, 16 arteries could be grouped into groups I and II consisting of eight each. Net p… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

1
20
0

Year Published

2004
2004
2011
2011

Publication Types

Select...
8

Relationship

2
6

Authors

Journals

citations
Cited by 27 publications
(21 citation statements)
references
References 32 publications
1
20
0
Order By: Relevance
“…45 There exists a positive correlation between intima/ media ratios and endogenous NOS inhibitors such as L-NMMA and asymmetric dimethylarginine in endothelial cells, as well as endothelin-1 within the vessel wall of perimenopausal human UtAs. 46 The reduction in intima/media ratios that we observed in our study may confer an arterioprotective effect on the UtAs.…”
Section: Discussionsupporting
confidence: 48%
“…45 There exists a positive correlation between intima/ media ratios and endogenous NOS inhibitors such as L-NMMA and asymmetric dimethylarginine in endothelial cells, as well as endothelin-1 within the vessel wall of perimenopausal human UtAs. 46 The reduction in intima/media ratios that we observed in our study may confer an arterioprotective effect on the UtAs.…”
Section: Discussionsupporting
confidence: 48%
“…According to the method described previously (6), pulmonary artery endothelial cells (PAECs) were collected by gently rubbing the luminal surface with each buffer specified for enzyme activity assays and Western blot analyses. To confirm the endothelial origin of the collected cells, they were stained using von Willebrand factor antibody (rabbit polyclonal antibody for human von Willebrand factor; Dako, Kyoto, Japan) and the labeled streptavidinbiotin staining reagent [LSAB kit/horseradish peroxidase (HRP); Dako] according to the method described previously (6). The population of von Willebrand factor-positive cells was determined as Ͼ98% of the collected cells.…”
Section: Methodsmentioning
confidence: 99%
“…Contents of MMA, ADMA, symmetric dimethylarginine (SDMA), and L-arginine in PAECs were determined by means of HPLC as reported previously (6,29). PAECs were collected from the pulmonary artery specimens by gently rubbing the luminal surface according to the method described previously (6).…”
Section: ϫ5mentioning
confidence: 99%
See 1 more Smart Citation
“…After thawing the specimens, endothelial cells were collected from each arterial specimen according to the method described previously (24,25). They were sonicated at 50 W, 28 kHz (GSD-50; SMT Co., Tokyo) 3 times for 15 s at 1-min intervals.…”
Section: Preparation Of Endothelial Cell Lysates and Smooth Muscle Lamentioning
confidence: 99%