Endogenous salicylic acid accumulation is required for chilling tolerance in cucumber (Cucumis sativus L.) seedlings

Dong, C.Y.; Li, Liang; Shang, Qingmao; Li, Xinyan; Zhang, Zhigang

doi:10.1007/s00425-014-2115-1

Cited by 121 publications

(80 citation statements)

References 54 publications

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“…Furthermore, although the number of studies is still very limited for plants in their natural habitat, highmountain plants tend to accumulate large amounts of ABA (Bano et al, 2009), a phytohormone that is known to mediate the acclimation/adaptation of plants to temperature extremes by modulating the up-and down-regulation of numerous genes (Gilmour and Thomashow, 1991). The activation of other chemical defenses, such as the accumulation of salicylates and jasmonates, which serve against biotrophs and necrotrophs, can also be affected by extreme temperatures (Kosova et al, 2012;Dong et al, 2014;Miura and Tada, 2014), but it has not been investigated thus far in high-mountain plants.…”

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confidence: 99%

Adaptation of the Long-Lived Monocarpic Perennial, Saxifraga longifolia to High Altitude

et al. 2016

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Global change is exerting a major effect on plant communities, altering their potential capacity for adaptation. Here, we aimed at unveiling mechanisms of adaptation to high altitude in an endemic long-lived monocarpic, Saxifraga longifolia, by combining demographic and physiological approaches. Plants from three altitudes (570, 1100, and 2100 m above sea level [a.s.l.]) were investigated in terms of leaf water and pigment contents, and activation of stress defense mechanisms. The influence of plant size on physiological performance and mortality was also investigated. Levels of photoprotective molecules (a-tocopherol, carotenoids, and anthocyanins) increased in response to high altitude (1100 relative to 570 m a.s.l.), which was paralleled by reduced soil and leaf water contents and increased ABA levels. The more demanding effect of high altitude on photoprotection was, however, partly abolished at very high altitudes (2100 m a.s.l.) due to improved soil water contents, with the exception of a-tocopherol accumulation. a-Tocopherol levels increased progressively at increasing altitudes, which paralleled with reductions in lipid peroxidation, thus suggesting plants from the highest altitude effectively withstood high light stress. Furthermore, mortality of juveniles was highest at the intermediate population, suggesting that drought stress was the main environmental driver of mortality of juveniles in this rocky plant species. Population structure and vital rates in the high population evidenced lower recruitment and mortality in juveniles, activation of clonal growth, and absence of plant sizedependent mortality. We conclude that, despite S. longifolia has evolved complex mechanisms of adaptation to altitude at the cellular, whole-plant and population levels, drought events may drive increased mortality in the framework of global change.

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confidence: 99%

Adaptation of the Long-Lived Monocarpic Perennial, Saxifraga longifolia to High Altitude

et al. 2016

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“…Another important ROS resource is NADPH oxidase. However, accumulating evidence has suggested that SA-mediated ROS production is independent of NADPH oxidase [25, 57]. Here, the SA-induced ROS was concluded to be generated from the imbalance between the photosynthetic electron transport and carbon fixation reaction, and this conclusion was confirmed by the light-dependency of ROS generation (Fig 4).…”

Section: Discussionmentioning

confidence: 59%

“…The quantitative determination and diaminobenzidine (DAB) histochemical staining of H 2 O 2 in cucumber leaves was performed as described previously [25]. …”

Section: Methodsmentioning

confidence: 99%

“…The intactness of the chloroplasts was approximately 85%, as shown by the Hill reaction [29]. The H 2 O 2 content was assayed in intact chloroplasts that were diluted 2-fold with 80% acetone and was measured by monitoring the A 410 of a titanium-peroxide complex as previously described [25]. The H 2 O 2 content was expressed in nanomoles per milligram of chlorophyll (nmol mg -1 Chl).…”

Section: Methodsmentioning

confidence: 99%

“…Young cucumber seedlings are susceptible to pathogen infections, cold, drought, and salinity stresses due to their low biomass, undeveloped protective structure, and large water and nutrient demands for growth and expansion. Exogenous SA can not only enhance seedling growth but also function as an “effective therapeutic agent” to protect cucumber against subsequent environmental stresses [23–25]. In our previous report, a 2-DE assay was carries out to reveal the SA-induced proteomic changes in cotyledons, and 59 SA-responsive proteins were successfully identified [26].…”

Section: Introductionmentioning

confidence: 99%

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Quantitative Proteomic Profiling of Early and Late Responses to Salicylic Acid in Cucumber Leaves

Dong

Cao

et al. 2016

PLoS ONE

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Salicylic acid (SA) is an important phytohormone that plays vital regulatory roles in plant growth, development, and stress responses. However, studies on the molecular mechanism of SA, especially during the early SA responses, are lagging behind. In this study, we initiated a comprehensive isobaric tag for relative and absolute quantitation (iTRAQ)-based proteomic analysis to explore the early and late SA-responsive proteins in leaves of cucumber (Cucumis sativus L.) seedlings. Upon SA application through the roots, endogenous SA accumulated in cucumber leaves. By assaying the changes in marker gene expression and photosynthetic rate, we collected samples at 12 h and 72 h post treatment (hpt) to profile the early and late SA responsiveness, respectively. The iTRAQ assay followed by tandem mass spectrometry revealed 135 differentially expressed proteins (DEPs) at 12 hpt and 301 DEPs at 72 hpt. The functional categories for these SA-responsive proteins included in a variety of biochemical processes, including photosynthesis, redox homeostasis, carbohydrate and energy metabolism, lipid metabolism, transport, protein folding and modification, proteolysis, cell wall organization, and the secondary phenylpropanoid pathway. Conclusively, based on the abundant changes of these DEPs, together with their putative functions, we proposed a possible SA-responsive protein network. It appears that SA could elicit reactive oxygen species (ROS) production via enhancing the photosynthetic electron transferring, and then confer some growth-promoting and stress-priming effects on cells during the late phase, including enhanced photosynthesis and ROS scavenging, altered carbon metabolic flux for the biosynthesis of amino acids and nucleotides, and cell wall reorganization. Overall, the present iTRAQ assay provides higher proteome coverage and deepened our understanding of the molecular basis of SA-responses.

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Salicylic Acid Signaling and its Role in Responses to Stresses in Plants

Zhao

Lü

Yang

2017

Mechanism of Plant Hormone Signaling Under Stress

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Endogenous salicylic acid accumulation is required for chilling tolerance in cucumber (Cucumis sativus L.) seedlings

Cited by 121 publications

References 54 publications

Adaptation of the Long-Lived Monocarpic Perennial, Saxifraga longifolia to High Altitude

Adaptation of the Long-Lived Monocarpic Perennial, Saxifraga longifolia to High Altitude

Quantitative Proteomic Profiling of Early and Late Responses to Salicylic Acid in Cucumber Leaves

Salicylic Acid Signaling and its Role in Responses to Stresses in Plants

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