Endogenous thiols and MRP transporters contribute to Hg2+ efflux in HgCl2-treated tubular MDCK cells

Molecular characterization and functions of zebrafish ABCC2 in cellular efflux of heavy metals

“…To survive various toxic environments, vertebrates including fish have developed defensive mechanisms against toxicants and MRP transporters appears to be the main factors involved in the multi-xenobiotic resistance developed during the evolution of aquatic organisms (Sauerborn Klobucar et al, 2010). Mammalian ABCC2s have been shown to be critical in the detoxification and elimination of heavy metals, including mercury (Aleo et al, 2005;Zalups and Bridges, 2010), arsenite (Carew and Leslie, 2010;Kala et al, 2000) and platinum (Guminski et al, 2006;Surowiak Livers and intestines were taken from 4 females, and kidneys were taken from 4 females and 4 males. Same tissues from different fish were mixed for isolation of total RNA and subjected to qRT-PCR analysis of abcc2 mRNA levels relative to the control.…”

“…Therefore, cells need effective detoxification mechanisms to survive the toxicity of these toxic metals. Previous studies have demonstrated that heavy metals can be detoxified and eliminated from cells by some of MRP transporters after conjugation to glutathione (Aleo et al, 2005;Chen et al, 1997;Ishikawa et al, 1994;Preveral et al, 2009). ABCC2/MRP2 has been shown to play important roles in the detoxification of heavy metals, including mercury (Aleo et al, 2005;Zalups and Bridges, 2010), arsenite (Carew and Leslie, 2010;Kala et al, 2000) and platinum (Guminski et al, 2006;Surowiak et al, 2006).…”

MRP proteins as potential mediators of heavy metal resistance in zebrafish cells

“…In addition to their role in drug resistance, MRPs play important functions in tissue defense to protect cells from the toxicity of various xenobiotics. Among MRP members, MRP1 confers resistance to a wide arrays of toxicants including heavy metals, such as arsenite (Broeks et al, 1996;Leslie et al, 2004), antimony (Leslie et al, 2004), cadmium (Broeks et al, 1996;Ishikawa et al, 1996), platinum (Ishikawa et al, 1996), mercury (Aleo et al, 2005), and zinc (Ishikawa et al, 1996). MRP2 is involved in the elimination and detoxification of arsenite (Kala et al, 2000;Lee et al, 2006), mercury (Bridges et al, 2008;Zalups and Bridges, 2009) and platinum (Surowiak et al, 2006).…”

“…It has been shown that ABC transporters such as PGP (ABCB1) and several MRPs (ABCCs) are involved in heavy metal excretion and detoxification (Aleo et al, 2005;Endo et al, 2002;Kimura et al, 2005aKimura et al, , 2005b. Metallothionein is an extensively distributed low molecular weight cysteine-rich intracellular metal binding protein for regulation of essential metals and detoxification of toxic heavy metals (Kagi and Schaffer, 1988).…”

Molecular analysis, developmental function and heavy metal-induced expression of ABCC5 in zebrafish

“…MRP transporters are able to efflux the glutathione conjugates of toxicants including heavy metals (Ishikawa et al, 1996;Aleo et al, 2005). Glutathione conjugates are generally regarded as products of phase II detoxification reaction, so MRPs are proposed to be the major factors of the multi-xenobiotics resistance (MXR) mechanism in aquatic organisms (Klobucar et al, 2005).…”