Endogenous Viral Genes in Thirteen Highly Inbred Chicken Lines and in Lines Selected for Immune Response Traits

Lamont, Susan J.; Chen, Y.; Aarts, H.J.M.; Arkel, M.C. van der Hulst-van; Beuving, G.; Leenstra, F.R.

doi:10.3382/ps.0710530

Cited by 41 publications

(35 citation statements)

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“…The F 1 population was produced in five hatches by crossing four outbred males of a broiler breeder male line [15] with dams of three highly inbred dam lines: one Fayoumi and two MHCcongenic Leghorn lines (G-B1 and G-B2) with inbreeding coefficients of 99% [38]. These inbred lines have been used in many studies of immune response and disease resistance [6,19,20,25]. The genetic distance between the parental lines maximized the feasibility of finding molecular genetic polymorphisms [38].…”

Section: Experimental Animalsmentioning

confidence: 99%

Association of INOS, TRAIL, TGF-β2, TGF-β3, and IgL genes with response to Salmonella enteritidis in poultry

Malek

Lamont

2003

Genet. Sel. Evol.

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-Several candidate genes were selected, based on their critical roles in the host's response to intracellular bacteria, to study the genetic control of the chicken response to Salmonella enteritidis (SE). The candidate genes were: inducible nitric oxide synthase (INOS), tumor necrosis factor related apoptosis inducing ligand (TRAIL), transforming growth factor β2 (TGF-β2), transforming growth factor β3 (TGF-β3), and immunoglobulin G light chain (IgL). Responses to pathogenic SE colonization or to SE vaccination were measured in the Iowa Salmonella response resource population (ISRRP). Outbred broiler sires and three diverse, highly inbred dam lines produced 508 F 1 progeny, which were evaluated as young chicks for either bacterial load isolated from spleen or cecum contents after pathogenic SE inoculation, or the circulating antibody level after SE vaccination. Fragments of each gene were sequenced from the founder lines of the resource population to identify genomic sequence variation. Single nucleotide polymorphisms (SNP) were identified, then PCR-RFLP techniques were developed to genotype the F 1 resource population. Linear mixed models were used for statistical analyses. Because the inbred dam lines always contributed one copy of the same allele, the heterozygous sire allele effects could be assessed in the F 1 generation. Association analyses revealed significant effects of the sire allele of TRAIL-StyI on the spleen (P < 0.07) and cecum (P < 0.0002) SE bacterial load. Significant effects (P < 0.04) were found on the cecum bacterial load for TGF-β3-BsrI. Varied and moderate association was found for SE vaccine antibody response for all genes. This is the first reported study on the association of SNP in INOS, TRAIL, TGF-β2, TGF-β3, and IgL with the chicken response to SE. Identification of candidate genes to improve the immune response may be useful for marker-assisted selection to enhance disease resistance.

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Section: Experimental Animalsmentioning

confidence: 99%

Association of INOS, TRAIL, TGF-β2, TGF-β3, and IgL genes with response to Salmonella enteritidis in poultry

Malek

Lamont

2003

Genet. Sel. Evol.

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“…Few such probes are currently available because they require cloning of flanking regions from genomic DNA of birds carrying only one ev insertion, and such birds are rare. An eul-specific probe showed that the genomic insertion site for eul was not occupied by a viral insert in Fayoumi chickens although they carried the restriction fragments usually associated with an eul occupied site in White Leghorns, ie 9.5 kb with SacI and 5.2 kb with BamHI (Lamont et al, 1992). A similar approach revealed a much lower frequency of occupation of the eul site in White Plymouth Rock chickens than in White Leghorns (Aarts et al, 1991).…”

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confidence: 97%

“…This observation suggested that ev genes expressing envelope antigens, such as ev6, may confer a selective advantage (Robinson et al, 1981), which could explain the ubiquity of ev6. However, ev6 was found to be associated with higher susceptibility to Marek's disease (Kuhnlein et al, 1989b) and lower immunoresponsiveness (Lamont et al, 1992). (Gavora et al, 1991) or to linkage with production trait genes for silent ev genes (Kuhnlein et al, 1989b).…”

mentioning

confidence: 99%

“…A classification of 22 different ev genes has been established from the study of restriction fragment length together with the determination of viral phenotypic expression (Smith, 1987;Crittenden, 1991 (Rovigatti and Astrin, 1983). Indeed, a very high level of polymorphism has been described for ev genes in commercial layers and broilers (Aarts et al, 1991;Boulliou et al, 1991;Iraqi et al, 1991;Sabour et al, 1992), and new ev genes were also found in other experimental strains (Gudkov et at, 1981;Ronfort et at, 1991;Lamont et al, 1992 (Merat and Bordas, 1982). The experimental Wyandotte strain (X55) was maintained for many generations as part of a conservation programme.…”

mentioning

confidence: 99%

“…The DNA was then blotted onto a charged nylon membrane (Biotrace, Gelman Labs) in 0.4 M NaOH. After transfer, the membranes were washed with 2 x SSPE (Maniatis et al, 1982) (Lamont et al, 1992). Birds free of ev genes have already been described in a White Leghorn background (Astrin et al, 1979;Gavora et al, 1989 (Iraqi et al, 1991).…”

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confidence: 99%

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Comparative analysis of avian leukosis virus-related endogenous viral genes in experimental strains of the domestic chicken

et al. 1994

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Association of MHC class I and class II gene polymorphisms with vaccine or challenge response to Salmonella enteritidis in young chicks

2002

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Salmonella enteritidis (SE) is a worldwide source of salmonellosis in humans, caused mainly by consumption of contaminated poultry products. The major histocompatibility complex (MHC) class I and class II molecules, which function as antigen-presentation structures, are involved in both macrophage and dendritic cell immune response to Salmonella and may, therefore, play an important role in host resistance to infections. The chicken MHC class I and class II were investigated as candidate genes for immune response to SE. We characterized the complete MHC class I cDNA sequences for an outbred broiler line and four diverse highly inbred lines: two MHC-congenic Leghorn (G-B2 and G-B1), one Egyptian Fayoumi (M15.2), and one Spanish (Sp21.1), to define the allelic sequences within these lines, so that we might study the association between particular MHC polymorphisms and response to SE. The F1 offspring of outbred broiler sires crossed with three inbred lines (G-B1, G-B2, and Fayoumi) were evaluated as young chicks for either bacterial load in spleen and cecum after pathogenic SE inoculation or antibody level after SE vaccination. Alleles defined by a Lys(148)-->Met(148) polymorphism in the MHC class I alpha(2) domain were associated with spleen bacterial load after SE challenge. These results suggest that particular MHC haplotypes may contribute to control of responses to SE, and that particular polymorphisms may serve as markers for genetic resistance to SE in the chicken.

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Endogenous Viral Genes in Thirteen Highly Inbred Chicken Lines and in Lines Selected for Immune Response Traits

Cited by 41 publications

References 20 publications

Association of INOS, TRAIL, TGF-β2, TGF-β3, and IgL genes with response to Salmonella enteritidis in poultry

Association of INOS, TRAIL, TGF-β2, TGF-β3, and IgL genes with response to Salmonella enteritidis in poultry

Comparative analysis of avian leukosis virus-related endogenous viral genes in experimental strains of the domestic chicken

Association of MHC class I and class II gene polymorphisms with vaccine or challenge response to Salmonella enteritidis in young chicks

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