Endogenous α-Ketol Linolenic Acid Levels in Short Day-Induced Cotyledons are Closely Related to Flower Induction in Pharbitis nil

Suzuki, Masayuki; Yamaguchi, Syuhei; Iida, Toshii; Hashimoto, Ikue; Teranishi, H; Mizoguchi, Masaya; Yano, Fumihiko; Todoroki, Yasushi; Watanabe, Naoharu; Yokoyama, Mineyuki

doi:10.1093/pcp/pcg007

Cited by 43 publications

(36 citation statements)

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“…We first observed changes in expression of these genes using RT-PCR between KODA-and mocktreated cotyledons, because the internal level of KODA is dramatically changed during the SD dark period (Suzuki et al 2003). However, none of these genes showed changes compared with the mock-treated cotyledons (Figure 3).…”

Section: Effects Of Koda Application On the Expression Of Flowering-rmentioning

confidence: 99%

“…Among them, a pair of cDNAs encoding PnFT1 and PnFT2 were isolated and it was demonstrated that products of these function as florigens in P. nil (Hayama et al 2007). Several floral stimuli in addition to florigen have been reported in P. nil (Hatayama and Takeno 2003;Ishioka et al 1990;Kong et al 2005;Shinozaki and Takimoto 1983;Suzuki et al 2003;Wada et al 2010). Among them, 9,10-ketol-octadecadienoic acid (KODA) is appropriate because endogenous levels of KODA in cotyledons are highly correlated with the mode of photoperiodic induction of flowering (Suzuki et al 2003).…”

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confidence: 99%

“…Several floral stimuli in addition to florigen have been reported in P. nil (Hatayama and Takeno 2003;Ishioka et al 1990;Kong et al 2005;Shinozaki and Takimoto 1983;Suzuki et al 2003;Wada et al 2010). Among them, 9,10-ketol-octadecadienoic acid (KODA) is appropriate because endogenous levels of KODA in cotyledons are highly correlated with the mode of photoperiodic induction of flowering (Suzuki et al 2003). Endogenous levels of KODA are elevated during the latter half of flower-inductive darkness, and floral induction can be canceled by the night-break treatment, that is a light exposure during the 8th hour of inductive darkness (Suzuki et al 2003).…”

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confidence: 99%

“…Among them, 9,10-ketol-octadecadienoic acid (KODA) is appropriate because endogenous levels of KODA in cotyledons are highly correlated with the mode of photoperiodic induction of flowering (Suzuki et al 2003). Endogenous levels of KODA are elevated during the latter half of flower-inductive darkness, and floral induction can be canceled by the night-break treatment, that is a light exposure during the 8th hour of inductive darkness (Suzuki et al 2003). KODA was originally isolated from Lemna paucicostata during experiments related to flowering and it showed flower-inducing activity when applied exogenously (Yamaguchi et al 2001;Yokoyama et al 2000).…”

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confidence: 99%

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Effects of 9,10-ketol-octadecadienoic acid (KODA) application on single and marginal short-day induction of flowering in Pharbitis nil cv. Violet

Ono

Kataoka

Yokoyama

et al. 2013

Plant Biotechnology

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Seedlings of the Japanese morning glory (Pharbitis nil, formerly Ipomoea nil) cv. Violet are induced to flower by a single short day treatment, and endogenous levels of 9,10-ketol-octadecadienoic acid (KODA) in cotyledons were found to correlate with this mode of short-day induction. When 100 µM KODA solution was sprayed on 7-day-old seedlings before and after a marginal short-day induction, the seedlings bore approximately 4 flower buds, a one-bud increase compared with control seedlings. In treated seedlings, the second node most commonly bore the first flower bud, one node lower than in the control seedlings. We then used RT-PCR to determine expression levels of 10 genes related to photoperiodic induction of flowering. In the cotyledons, no differences were observed in expression of any genes, including P. nil FLOWERING TIME LOCUS T (PnFT1 and PnFT2), between the KODA-treated and the control seedlings. In the apical buds, P. nil APETALA1 (PnAP1) was expressed earlier in the KODA-treated seedlings than in the control seedlings. A decrease in the expression of P. nil TERMINAL FLOWER1b (PnTFL1b) was also observed in the KODA-treated seedlings. These results suggest that KODA acts as a weak enhancer of flower bud formation.

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Section: Effects Of Koda Application On the Expression Of Flowering-rmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Effects of 9,10-ketol-octadecadienoic acid (KODA) application on single and marginal short-day induction of flowering in Pharbitis nil cv. Violet

Ono

Kataoka

Yokoyama

et al. 2013

Plant Biotechnology

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“…Flowers forming in May were used for the experiments. Pharbitis nil 'Violet' was germinated and cultivated in liquid medium as reported before (Suzuki et al 2003). Germinated seedlings were grown on wet sea sand under continuous light (the irradiance and temperature were the same as those used for the cultivation following dark exposure; see below) for 4 days.…”

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confidence: 99%