Endomembrane Dissection Using Chemically Induced Bioactive Clusters

Worden, Natasha; Girke, Thomas; Drakakaki, Georgia

doi:10.1007/978-1-62703-592-7_16

Cited by 5 publications

(1 citation statement)

References 21 publications

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“…The sensitivity to drugs can be used to discriminate between different membrane compartments (Geldner et al, 2003;Dettmer et al, 2006;Jaillais et al, 2006;Jaillais et al, 2008;Worden et al, 2014;Kania et al, 2018;Mishev et al, 2018), but colocalization is the gold standard.…”

Section: Imaging Intracellular Trafficking Fast and Tiny!mentioning

confidence: 99%

Imaging the living plant cell: From probes to quantification

et al. 2021

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At the center of cell biology is our ability to image the cell and its various components, either in isolation or within an organism. Given its importance, biological imaging has emerged as a field of its own, which is inherently highly interdisciplinary. Indeed, biologists rely on physicists and engineers to build new microscopes and imaging techniques, chemists to develop better imaging probes, and mathematicians and computer scientists for image analysis and quantification. Live imaging collectively involves all the techniques aimed at imaging live samples. It is a rapidly evolving field, with countless new techniques, probes, and dyes being continuously developed. Some of these new methods or reagents are readily amenable to image plant samples, while others are not and require specific modifications for the plant field. Here, we review some recent advances in live imaging of plant cells. In particular, we discuss the solutions that plant biologists use to live image membrane-bound organelles, cytoskeleton components, hormones, and the mechanical properties of cells or tissues. We not only consider the imaging techniques per se, but also how the construction of new fluorescent probes and analysis pipelines are driving the field of plant cell biology.

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Section: Imaging Intracellular Trafficking Fast and Tiny!mentioning

confidence: 99%

Imaging the living plant cell: From probes to quantification

et al. 2021

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Using Chemical Genomics to Study Cell Wall Formation and Cell Growth in Arabidopsis thaliana and Penium margaritaceum

Worden

Esteve

Domozych

et al. 2014

Methods in Molecular Biology

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The cell wall is directly involved in cell growth, and its ability to loosen and rearrange allows for cell expansion through the existing turgor pressure. Thus, information on cell wall deposition and rearrangement can provide insights into the overall plant growth. This chapter describes two methods that can be used to evaluate cell expansion (1) in the model plant Arabidopsis thaliana and (2) the model alga Penium margaritaceum. These methods are further used to screen for small molecules that induce cell growth phenotypic changes affecting cell wall. Identification of such small molecules is beneficial due to their posttranslational mechanism of action that can be controlled in a temporal and spatial manner. Chemical genomics has the ability to overcome issues of genetic redundancy and lethality, which can hinder traditional genetic methods. The identification of small molecules in these screens will provide useful information on plant cell wall biology and overall plant growth.

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Unconventional Protein Secretion in Plants

Davis

Kang

Heringer

et al. 2016

Unconventional Protein Secretion

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Unconventional protein secretion (UPS) describes secretion pathways that bypass one or several of the canonical secretion pit-stops on the way to the plasma membrane, and/or involve the secretion of leaderless proteins. So far, alternatives to conventional secretion were primarily observed and studied in yeast and animal cells. The sessile lifestyle of plants brings with it unique restraints on how they adapt to adverse conditions and environmental challenges. Recently, attention towards unconventional secretion pathways in plant cells has substantially increased, with the large number of leaderless proteins identified through proteomic studies. While UPS pathways in plants are certainly not yet exhaustively researched, an emerging notion is that induction of UPS pathways is correlated with pathogenesis and stress responses. Given the multitude UPS events observed, comprehensively organizing the routes proteins take to the apoplast in defined UPS categories is challenging. With the establishment of a larger collection of studied plant proteins taking these UPS pathways, a clearer picture of endomembrane trafficking as a whole will emerge. There are several novel enabling technologies, such as vesicle proteomics and chemical genomics, with great potential for dissecting secretion pathways, providing information about the cargo that travels along them and the conditions that induce them.

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Endomembrane Dissection Using Chemically Induced Bioactive Clusters

Cited by 5 publications

References 21 publications

Imaging the living plant cell: From probes to quantification

Imaging the living plant cell: From probes to quantification

Using Chemical Genomics to Study Cell Wall Formation and Cell Growth in Arabidopsis thaliana and Penium margaritaceum

Unconventional Protein Secretion in Plants

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