Endometrial and luteal gene expression of putative gene regulators of the equine maternal recognition of pregnancy

Amorim, Mariana Diel de; Bramer, Sarah A.; Rajamanickam, Gayathri D.; Klein, Claudia; Card, Claire

doi:10.1016/j.anireprosci.2022.107064

Cited by 5 publications

(5 citation statements)

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“…In luteal tissue, LNPEP abundance was low on D8 then increased in both groups on D10 and D12 and remained high on D15 diestrus. 41 Consistent with the presence of endometrial and luteal LNPEP , we reported that LNPEP is present in a wide variety of tissues including the equine placenta. There was a difference in placental LNPEP with the body and pregnant horn having higher levels than the non-pregnant horn.…”

Section: Discussionsupporting

confidence: 71%

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Characterization of serum and tissue oxytocinase and tissue oxytocin in the pregnant and non-pregnant mare

Amorim

Dong

Byron

et al. 2023

Sci Rep

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Oxytocin is a hormone with functions in: reproduction, maternal bonding, milk ejection, and feeding/social behavior, and is reported to be present in a variety of tissues. Our goal is to characterize oxytocin and leucyl and cystinyl aminopeptidase (LNPEP/oxytocinase), a key regulator of oxytocin in mares. We measured serum and tissue LNPEP by ELISA from ovulation (D0) until D21–22 in non-pregnant (n = 5) and pregnant mares (n = 6); and in periparturient and postpartum mares (n = 18). Placenta (n = 7) and homogenized tissue of diestrus mares (n = 6) were evaluated using protein determinations and LNPEP ELISAs. Identification of LNPEP and OXT protein in tissues was also performed via western blot, immunohistochemistry and liquid chromatography-mass spectrometry (LC-MS/MS). Furthermore, in situ hybridization was performed for LNPEP and OXT on endometrium, myometrium, pituitary and corpus luteum (CL). Serum LNPEP concentration were similar. Placental LNPEP U/mg protein was highest in the body and pregnant horn. The highest to lowest LNPEP U/mg protein by tissue were: myometrium > follicle wall > endometrium > kidney > CL > liver. Oxytocin was identified in the equine pituitary, CL and placenta and is likely to act in autocrine or paracrine manner, while LNPEP may act systemically and locally to regulate the availability of OXT.

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Section: Discussionsupporting

confidence: 71%

“…Equine endometrial and luteal OXT in early pregnancy has been reported showing changes in abundance based on day post ovulation and pregnancy status 41 . The highest OXT endometrial abundance was reported on day 10 where pregnant mares had greater level than diestrus or pregnant mares on D8 and D15.…”

Section: Discussionmentioning

confidence: 99%

Characterization of serum and tissue oxytocinase and tissue oxytocin in the pregnant and non-pregnant mare

Amorim

Dong

Byron

et al. 2023

Sci Rep

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“… 12 , 13 , 32 , 33 It also degraded peptide hormones such as oxytocin, vasopressin and angiotensin III, and played a role in maintaining homeostasis during pregnancy. 12 , 34 Blockade of LNPEP-angiotensin IV signaling reactivated the neuronal peptides in the brain and cleaves Met-enkephalin and dynorphin. 10 However, how LNPEP might be correlated with OV immune microenvironment and lead to tumor heterogeneity has not been fully clarified.…”

Section: Discussionmentioning

confidence: 99%

Leucyl and Cystinyl Aminopeptidase as a Prognostic-Related Biomarker in OV Correlating with Immune Infiltrates

Ma¹,

Chang²,

Wang³

et al. 2023

PGPM

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Background It was indicated that tumor intrinsic heterogeneity and the tumor microenvironment (TME) of ovarian cancer (OV) influence immunotherapy efficacy and patient outcomes. Leucyl and cystinyl aminopeptidase (LNPEP) encodes a zinc-dependent aminopeptidase, which has been proved to participant in the vesicle-mediated transport and class I MHC mediated antigen processing and presentation. However, the function of LNPEP in TME of OV and its potential molecular mechanisms have not been determined. Therefore, we aimed to investigate a prognostic biomarker which may be helpful in identifying TME heterogeneity of ovarian cancer. Methods In this study, bioinformatics databases were used to explore the expression profile and immune infiltration of LNPEP. Bioinformatics analyses of survival data and interactors of LNPEP were conducted to predict the prognostic value of LNPEP in OV. The protein levels of LNPEP were validated by Western blot and immunohistochemistry. Results Based on the TCGA data, our data displayed that the mRNA expression of LNPEP was markedly down-regulated in ovarian cancer than that in para-cancer tissues, contrary to the protein level. Importantly, high LNPEP expression was associated with poor prognosis in patients with OV. Furthermore, Cox regression analysis showed that LNPEP was an independent prognostic factor in OV. GO and KEGG pathway analyses indicated the co-expressed genes of LNPEP were mainly related to a variety of immune-related pathways, including Th1 and Th2 cell differentiation, Th17 cell differentiation, and immunoregulatory interaction. Our data also demonstrated that the expression of LNPEP was strongly correlated with immune infiltration levels, immunomodulators, chemokines and chemokine receptors. Conclusion In our study, we identified and established a prognostic signature of immune-related LNPEP in OV, which will be of great value in predicting the prognosis of clinical trials and may become a new therapeutic target for immunological research and potential prognostic biomarker in OV.

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“…Both endometrial and luteal oxytocin concentrations show changes based on the pregnancy status and the day post-ovulation [13], while around the timing of MRP the transcriptional levels of OXTR are similar between pregnant and non-pregnant mares [14,15]. However, OXTR protein levels decrease in pregnant animals [11][12][13], suggesting that OXTR are modulated at the post-transcription level rather than at the transcriptional one. In cycling mares, OXTR concentrations increase approximately threefold on day 14 compared to days 10 and 18, with no such increase seen in pregnant animals [12], suggesting that suppression of the expression of OXTR needs to be initiated before day 14 in pregnant mares.…”

Section: Introductionmentioning

confidence: 98%

“…Oxytocin also plays a role in the luteolytic cascade, with the binding of endometrial oxytocin to its receptor (OXTR) in the endometrium believed to cause the pulsatile release of PGF2α [11,12]. Both endometrial and luteal oxytocin concentrations show changes based on the pregnancy status and the day post-ovulation [13], while around the timing of MRP the transcriptional levels of OXTR are similar between pregnant and non-pregnant mares [14,15]. However, OXTR protein levels decrease in pregnant animals [11][12][13], suggesting that OXTR are modulated at the post-transcription level rather than at the transcriptional one.…”

Section: Introductionmentioning

confidence: 99%

The Timing of the Maternal Recognition of Pregnancy Is Specific to Individual Mares

Newcombe¹,

Cuervo‐Arango

Wilsher

2023

Animals

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The present experiment aimed at determining whether the timing of the maternal recognition of pregnancy (MRP) was specific to individual mares by determining when luteostasis, a failure to return to oestrus, reliably occurred in individuals following embryo reduction. Singleton (n = 150) and synchronous twin pregnancies (n = 9) were reduced in 10 individuals (5–29 reductions/mare) at pre-determined time points within days 10 (n = 20), 11 (n = 65), 12 (n = 47), 13 (n = 12) or 14 (n = 15) of pregnancy. Prior to embryo reduction, the vesicle diameter was measured in 71% (106/150) of the singleton pregnancies. The interovulatory interval (IOI) was recorded on 78 occasions in seven of the mares in either non-pregnant cycles (n = 37) or those in which luteolysis followed embryo reduction (n = 41). The earliest time post-ovulation at which the embryo reduction resulted in luteostasis in an individual was 252 h (mid-Day 10). Consistency in luteostasis following embryo reduction showed individual variation between mares (272–344 h). Binary logistic regression analysis showed an individual mare effect (p < 0.001) and an effect of the interval post-ovulation at which embryo reduction was undertaken (p < 0.001). However, there was no significant effect of vesicle diameter at the time of embryo reduction (p = 0.099), nor a singleton or twin pregnancy (p = 0.993), on the dependent of luteolysis or luteostasis. The median IOI between individual mares varied significantly (p < 0.05) but was not correlated to the timing of MRP. The timing of MRP varied between the mares but was repeatable in each individual. The factors and mechanisms underlying the individuality in the timing of MRP were not determined and warrant further study.

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Endometrial and luteal gene expression of putative gene regulators of the equine maternal recognition of pregnancy

Cited by 5 publications

References 62 publications

Characterization of serum and tissue oxytocinase and tissue oxytocin in the pregnant and non-pregnant mare

Characterization of serum and tissue oxytocinase and tissue oxytocin in the pregnant and non-pregnant mare

Leucyl and Cystinyl Aminopeptidase as a Prognostic-Related Biomarker in OV Correlating with Immune Infiltrates

The Timing of the Maternal Recognition of Pregnancy Is Specific to Individual Mares

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